Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Mycobacterium tuberculosis |
Crystallization (Comment) | Organism |
---|---|
wild-type and mutant D151N/E222Q in various complexes with Mn2+, NADPH, and inhibotr fosmidomycin. Asymmetric unit corresponds to biological homodimer. Crystal contacts stabilize an open active site in the B molecule, the A molecule displays closed conformation | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
D151N/E222Q | loss of catalytic ability, loss of binding of Mn2+. Crystallization data | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
fosmidomycin | - |
Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41700 | - |
2 * 41700, calculated and crystallization data | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WNS1 | - |
- |
Mycobacterium tuberculosis H37Rv | P9WNS1 | - |
- |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 41700, calculated and crystallization data | Mycobacterium tuberculosis |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00008 | - |
pH 7.5, 22°C | Mycobacterium tuberculosis | fosmidomycin |