Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Methanocaldococcus jannaschii |
expression in Escherichia coli | Methanothermus fervidus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
3-sulfopyruvate | cosubstrate NADH, pH 8.0, 70°C | Methanocaldococcus jannaschii | |
0.07 | - |
3-sulfopyruvate | cosubstrate NADH, pH 8.0, 70°C | Methanothermus fervidus | |
0.11 | - |
oxaloacetate | cosubstrate NADH, pH 8.0, 70°C | Methanothermus fervidus | |
0.13 | - |
oxaloacetate | cosubstrate NADH, pH 8.0, 70°C | Methanocaldococcus jannaschii | |
0.21 | - |
3-sulfopyruvate | cosubstrate NADPH, pH 8.0, 70°C | Methanocaldococcus jannaschii | |
0.21 | - |
3-sulfopyruvate | cosubstrate NADPH, pH 8.0, 70°C | Methanothermus fervidus | |
0.95 | - |
oxaloacetate | cosubstrate NADPH, pH 8.0, 70°C | Methanothermus fervidus | |
1.9 | - |
2-oxoglutarate | cosubstrate NADH, pH 8.0, 70°C | Methanocaldococcus jannaschii | |
5.32 | - |
oxaloacetate | cosubstrate NADPH, pH 8.0, 70°C | Methanocaldococcus jannaschii | |
15 | - |
1-oxo-1,3,4,6-hexanetetracarboxylate | cosubstrate NADH, pH 8.0, 70°C | Methanocaldococcus jannaschii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | Q58820 | - |
- |
Methanothermus fervidus | P16142 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R)-3-sulfolactate + NAD+ | - |
Methanocaldococcus jannaschii | 3-sulfopyruvate + NADH + H+ | - |
r | |
(2R)-3-sulfolactate + NAD+ | - |
Methanothermus fervidus | 3-sulfopyruvate + NADH + H+ | - |
r | |
(S)-2-hydroxyglutarate + NAD+ | - |
Methanocaldococcus jannaschii | 2-oxoglutarate + NADH + H+ | - |
r | |
(S)-lactate + NAD+ | - |
Methanocaldococcus jannaschii | pyruvate + NADH + H+ | - |
r | |
(S)-lactate + NAD+ | - |
Methanothermus fervidus | pyruvate + NADH + H+ | - |
r | |
1-hydroxy-1,3,4,6-hexanetetracarboxylate + NAD+ | - |
Methanocaldococcus jannaschii | 1-oxo-1,3,4,6-hexanetetracarboxylate + NADH + H+ | - |
r | |
3-sulfopyruvate + NADH + H+ | - |
Methanocaldococcus jannaschii | (S)-3-sulfolactate + NAD+ | - |
r | |
3-sulfopyruvate + NADH + H+ | - |
Methanothermus fervidus | (S)-3-sulfolactate + NAD+ | - |
r | |
additional information | enzyme additionally functions as malate dehydrogenase, reducing oxalacetate to (S)-malate using either NADH or NADPH as a reductant. No substrate: 2-oxoglutarate, pyruvate, 1-oxo-1,3,4,6-hexanetetracarboxylate | Methanothermus fervidus | ? | - |
? | |
additional information | enzyme additionally functions as malate dehydrogenase, reducing oxalacetate to (S)-malate using either NADH or NADPH as a reductant. No substrate: pyruvate | Methanocaldococcus jannaschii | ? | - |
? | |
oxaloacetate + NADH + H+ | - |
Methanocaldococcus jannaschii | (S)-malate + NAD+ | - |
? | |
oxaloacetate + NADH + H+ | - |
Methanothermus fervidus | (S)-malate + NAD+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MJ1425 | - |
Methanocaldococcus jannaschii |
MJ1425 | - |
Methanothermus fervidus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Methanothermus fervidus | |
NADH | ratio vmax/Km for NADH is about 20fold greater than for NADPH | Methanocaldococcus jannaschii | |
NADPH | - |
Methanothermus fervidus | |
NADPH | ratio vmax/Km for NADH is about 20fold greater than for NADPH | Methanocaldococcus jannaschii |
General Information | Comment | Organism |
---|---|---|
physiological function | enzyme is likely to be involved in the biosynthesis of both coenzyme M and methanopterin | Methanocaldococcus jannaschii |
physiological function | enzyme is likely to be involved in the biosynthesis of both coenzyme M and methanopterin | Methanothermus fervidus |