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Literature summary for 1.1.1.343 extracted from

  • Rauch, B.; Pahlke, J.; Schweiger, P.; Deppenmeier, U.
    Characterization of enzymes involved in the central metabolism of Gluconobacter oxydans (2010), Appl. Microbiol. Biotechnol., 88, 711-718.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.064
-
NAD+ in 40 mM potassium phosphate buffer, at pH 7.0 and 25┬░C Gluconobacter oxydans
0.44
-
6-phospho-D-gluconate in 40 mM potassium phosphate buffer, at pH 7.0 and 25┬░C Gluconobacter oxydans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35900
-
4 * 35900, SDS-PAGE Gluconobacter oxydans
135000
-
native PAGE Gluconobacter oxydans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6-phospho-D-gluconate + NAD+ Gluconobacter oxydans
-
D-ribulose 5-phosphate + CO2 + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Gluconobacter oxydans G5EBD7
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-phospho-D-gluconate + NAD+
-
Gluconobacter oxydans D-ribulose 5-phosphate + CO2 + NADH + H+
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 35900, SDS-PAGE Gluconobacter oxydans

Synonyms

Synonyms Comment Organism
6PGDH
-
Gluconobacter oxydans
Gox1705
-
Gluconobacter oxydans
phosphogluconate dehydrogenase
-
Gluconobacter oxydans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
23
-
6-phospho-D-gluconate in 40 mM potassium phosphate buffer, at pH 7.0 and 25┬░C Gluconobacter oxydans

Cofactor

Cofactor Comment Organism Structure
NAD+ dependent on Gluconobacter oxydans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
54.5
-
6-phospho-D-gluconate in 40 mM potassium phosphate buffer, at pH 7.0 and 25┬░C Gluconobacter oxydans