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Literature summary for 1.1.1.346 extracted from

  • Maremonti, M.; Greco Jr., G.; Wichmann, R.
    Characterisation of 2,5-diketo-D-gluconic acid reductase from Corynebacterium sp. (1996), Biotechnol. Lett., 18, 845-850.
No PubMed abstract available
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
19.78
-
 2,5-didehydro-D-gluconate at pH 6.4 and 25°C Corynebacterium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 4 * 35000, SDS-PAGE Corynebacterium sp.
140000
-
 gel filtration Corynebacterium sp.

Organism

Organism UniProt Comment Textmining
Corynebacterium sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Sephacel column chromatography and Cibacron blue 3GA column chromatography Corynebacterium sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0276
-
 crude extract, at pH 6.4 and 25°C Corynebacterium sp.
1.967
-
 after 71.03fold purification, at pH 6.4 and 25°C Corynebacterium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,5-didehydro-D-gluconate + NADPH + H+
-
 Corynebacterium sp. 2-dehydro-L-gulonate + NADP+
-
 ?

Subunits

Subunits Comment Organism
homotetramer 4 * 35000, SDS-PAGE Corynebacterium sp.

Synonyms

Synonyms Comment Organism
2,5-diketo-D-gluconic acid reductase
-
 Corynebacterium sp.
2,5-DKG reductase
-
 Corynebacterium sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 80 after approximately 10 min of deactivation, enzyme activity attains a constant value, independently of deactivation temperature (40-80°C). This value corresponds to the 30% of native activity Corynebacterium sp.

pI Value

Organism Comment pI Value Maximum pI Value
Corynebacterium sp. isoelectric focusing
-
 4.7