KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.051 | - |
geraniol | in 50 mM Tris-HCl (pH 8.5), at 22°C | Carpoglyphus lactis | |
0.0595 | - |
NAD+ | in 50 mM Tris-HCl (pH 8.5), at 22°C | Carpoglyphus lactis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40630 | - |
1 * 40630, calculated from amino acid sequence | Carpoglyphus lactis |
42800 | - |
1 * 42800, active form, SDS-PAGE | Carpoglyphus lactis |
56200 | - |
gel filtration | Carpoglyphus lactis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
geraniol + NAD+ | Carpoglyphus lactis | the enzyme selectively oxidizes geraniol | geranial + NADH + H+ | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Carpoglyphus lactis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, Toyopearl phenyl-650M column chromatography, Toyopearl HW-50F column chromatography, DEAE-cellulose column chromatography, Blue-Cellulofine column chromatography, MonoQ column chromatography and Superdex 200gel filtration | Carpoglyphus lactis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.016 | - |
crude enzyme, in 50 mM Tris-HCl (pH 8.5), at 22°C | Carpoglyphus lactis |
31 | - |
after 1879fold purification, in 50 mM Tris-HCl (pH 8.5), at 22°C | Carpoglyphus lactis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesol + NAD+ | 50% activity compared to geraniol | Carpoglyphus lactis | (2E,6E)-farnesyl aldehyde + NADH + H+ | - |
? | |
3-methyl-2-buten-1-ol + NAD+ | 4.3% activity compared to geraniol | Carpoglyphus lactis | 3-methyl-but-2-enal + NADH + H+ | - |
? | |
citronellol + NAD+ | 4.3% activity compared to geraniol | Carpoglyphus lactis | citronellal + NADH + H+ | - |
? | |
geraniol + NAD+ | the enzyme selectively oxidizes geraniol | Carpoglyphus lactis | geranial + NADH + H+ | - |
ir | |
additional information | NADP+ is ineffective as a cofactor. The enzyme does not use nerol as substrate. Straight-chain alcohols such as 1-octanol, 2-buten-1-ol, 1-butanol and ethanol serve as poor substrates | Carpoglyphus lactis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 40630, calculated from amino acid sequence | Carpoglyphus lactis |
monomer | 1 * 42800, active form, SDS-PAGE | Carpoglyphus lactis |
Synonyms | Comment | Organism |
---|---|---|
GeDH | - |
Carpoglyphus lactis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Carpoglyphus lactis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2996 | - |
geraniol | in 50 mM Tris-HCl (pH 8.5), at 22°C | Carpoglyphus lactis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Carpoglyphus lactis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | as the pH increases from 7.0 to 7.5, there is a sharp increase in enzyme activity whereas the activity decreases sharply beyond pH 10.0 | Carpoglyphus lactis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | NADP+ is ineffective as cofactor | Carpoglyphus lactis |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme plays an important role in the biosynthesis of neral, an alarm pheromone in Carpoglyphus lactis | Carpoglyphus lactis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
58800 | - |
geraniol | in 50 mM Tris-HCl (pH 8.5), at 22°C | Carpoglyphus lactis |