BRENDA - Enzyme Database
show all sequences of 1.1.1.347

Geraniol dehydrogenase, the key enzyme in biosynthesis of the alarm pheromone, from the astigmatid mite Carpoglyphus lactis (Acari: Carpoglyphidae)

Noge, K.; Kato, M.; Mori, N.; Kataoka, M.; Tanaka, C.; Yamasue, Y.; Nishida, R.; Kuwahara, Y.; FEBS J. 275, 2807-2817 (2008)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.051
-
geraniol
in 50 mM Tris-HCl (pH 8.5), at 22C
Carpoglyphus lactis
0.0595
-
NAD+
in 50 mM Tris-HCl (pH 8.5), at 22C
Carpoglyphus lactis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40630
-
1 * 40630, calculated from amino acid sequence
Carpoglyphus lactis
42800
-
1 * 42800, active form, SDS-PAGE
Carpoglyphus lactis
56200
-
gel filtration
Carpoglyphus lactis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
geraniol + NAD+
Carpoglyphus lactis
the enzyme selectively oxidizes geraniol
geranial + NADH + H+
-
-
ir
Organism
Organism
UniProt
Commentary
Textmining
Carpoglyphus lactis
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
ammonium sulfate precipitation, Toyopearl phenyl-650M column chromatography, Toyopearl HW-50F column chromatography, DEAE-cellulose column chromatography, Blue-Cellulofine column chromatography, MonoQ column chromatography and Superdex 200gel filtration
Carpoglyphus lactis
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.016
-
crude enzyme, in 50 mM Tris-HCl (pH 8.5), at 22C
Carpoglyphus lactis
31
-
after 1879fold purification, in 50 mM Tris-HCl (pH 8.5), at 22C
Carpoglyphus lactis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(2E,6E)-farnesol + NAD+
50% activity compared to geraniol
686700
Carpoglyphus lactis
(2E,6E)-farnesyl aldehyde + NADH + H+
-
-
-
?
3-methyl-2-buten-1-ol + NAD+
4.3% activity compared to geraniol
686700
Carpoglyphus lactis
3-methyl-but-2-enal + NADH + H+
-
-
-
?
citronellol + NAD+
4.3% activity compared to geraniol
686700
Carpoglyphus lactis
citronellal + NADH + H+
-
-
-
?
geraniol + NAD+
the enzyme selectively oxidizes geraniol
686700
Carpoglyphus lactis
geranial + NADH + H+
-
-
-
ir
additional information
NADP+ is ineffective as a cofactor. The enzyme does not use nerol as substrate. Straight-chain alcohols such as 1-octanol, 2-buten-1-ol, 1-butanol and ethanol serve as poor substrates
686700
Carpoglyphus lactis
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
monomer
1 * 40630, calculated from amino acid sequence; 1 * 42800, active form, SDS-PAGE
Carpoglyphus lactis
Synonyms
Synonyms
Commentary
Organism
GeDH
-
Carpoglyphus lactis
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
25
-
-
Carpoglyphus lactis
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2996
-
geraniol
in 50 mM Tris-HCl (pH 8.5), at 22C
Carpoglyphus lactis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
-
Carpoglyphus lactis
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7
10
as the pH increases from 7.0 to 7.5, there is a sharp increase in enzyme activity whereas the activity decreases sharply beyond pH 10.0
Carpoglyphus lactis
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
NADP+ is ineffective as cofactor
Carpoglyphus lactis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
NADP+ is ineffective as cofactor
Carpoglyphus lactis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.051
-
geraniol
in 50 mM Tris-HCl (pH 8.5), at 22C
Carpoglyphus lactis
0.0595
-
NAD+
in 50 mM Tris-HCl (pH 8.5), at 22C
Carpoglyphus lactis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40630
-
1 * 40630, calculated from amino acid sequence
Carpoglyphus lactis
42800
-
1 * 42800, active form, SDS-PAGE
Carpoglyphus lactis
56200
-
gel filtration
Carpoglyphus lactis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
geraniol + NAD+
Carpoglyphus lactis
the enzyme selectively oxidizes geraniol
geranial + NADH + H+
-
-
ir
Purification (Commentary) (protein specific)
Commentary
Organism
ammonium sulfate precipitation, Toyopearl phenyl-650M column chromatography, Toyopearl HW-50F column chromatography, DEAE-cellulose column chromatography, Blue-Cellulofine column chromatography, MonoQ column chromatography and Superdex 200gel filtration
Carpoglyphus lactis
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.016
-
crude enzyme, in 50 mM Tris-HCl (pH 8.5), at 22C
Carpoglyphus lactis
31
-
after 1879fold purification, in 50 mM Tris-HCl (pH 8.5), at 22C
Carpoglyphus lactis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(2E,6E)-farnesol + NAD+
50% activity compared to geraniol
686700
Carpoglyphus lactis
(2E,6E)-farnesyl aldehyde + NADH + H+
-
-
-
?
3-methyl-2-buten-1-ol + NAD+
4.3% activity compared to geraniol
686700
Carpoglyphus lactis
3-methyl-but-2-enal + NADH + H+
-
-
-
?
citronellol + NAD+
4.3% activity compared to geraniol
686700
Carpoglyphus lactis
citronellal + NADH + H+
-
-
-
?
geraniol + NAD+
the enzyme selectively oxidizes geraniol
686700
Carpoglyphus lactis
geranial + NADH + H+
-
-
-
ir
additional information
NADP+ is ineffective as a cofactor. The enzyme does not use nerol as substrate. Straight-chain alcohols such as 1-octanol, 2-buten-1-ol, 1-butanol and ethanol serve as poor substrates
686700
Carpoglyphus lactis
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 40630, calculated from amino acid sequence; 1 * 42800, active form, SDS-PAGE
Carpoglyphus lactis
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
25
-
-
Carpoglyphus lactis
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2996
-
geraniol
in 50 mM Tris-HCl (pH 8.5), at 22C
Carpoglyphus lactis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
-
Carpoglyphus lactis
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7
10
as the pH increases from 7.0 to 7.5, there is a sharp increase in enzyme activity whereas the activity decreases sharply beyond pH 10.0
Carpoglyphus lactis
General Information
General Information
Commentary
Organism
physiological function
the enzyme plays an important role in the biosynthesis of neral, an alarm pheromone in Carpoglyphus lactis
Carpoglyphus lactis
General Information (protein specific)
General Information
Commentary
Organism
physiological function
the enzyme plays an important role in the biosynthesis of neral, an alarm pheromone in Carpoglyphus lactis
Carpoglyphus lactis
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
58800
-
geraniol
in 50 mM Tris-HCl (pH 8.5), at 22C
Carpoglyphus lactis
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
58800
-
geraniol
in 50 mM Tris-HCl (pH 8.5), at 22C
Carpoglyphus lactis
Other publictions for EC 1.1.1.347
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
718643
Lueddeke
Geraniol and geranial dehydrog ...
Castellaniella defragrans, Castellaniella defragrans 65Phen
Appl. Environ. Microbiol.
78
2128-2136
2012
-
-
1
-
-
-
4
14
-
1
5
2
-
2
-
-
1
-
-
-
2
-
20
2
3
-
-
-
-
1
-
-
3
-
-
-
-
-
1
3
-
-
-
-
4
-
14
-
1
5
2
-
-
-
1
-
-
2
-
20
2
-
-
-
-
1
-
-
-
1
-
-
1
12
12
686700
Noge
Geraniol dehydrogenase, the ke ...
Carpoglyphus lactis
FEBS J.
275
2807-2817
2008
-
-
-
-
-
-
-
2
-
-
3
1
-
1
-
-
1
-
-
-
2
-
5
1
1
1
-
-
1
1
1
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
-
3
1
-
-
-
1
-
-
2
-
5
1
1
-
-
1
1
1
-
-
-
1
1
-
1
1