BRENDA - Enzyme Database
show all sequences of 1.1.1.347

Geraniol and geranial dehydrogenases induced in anaerobic monoterpene degradation by Castellaniella defragrans

Lueddeke, F.; Wuelfing, A.; Timke, M.; Germer, F.; Weber, J.; Dikfidan, A.; Rahnfeld, T.; Linder, D.; Meyerdierks, A.; Harder, J.; Appl. Environ. Microbiol. 78, 2128-2136 (2012)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expressed in Escherichia coli strain BL21 Star(DE3); expression in Escherichia coli
Castellaniella defragrans
Inhibitors
Inhibitors
Commentary
Organism
Structure
1-cyclohexyl-N-(2-morpholinoethyl)carbodiimide
I80 of less than 34 mM
Castellaniella defragrans
diethylpyrocarbonate
I80 of less than 17 mM
Castellaniella defragrans
EDTA
7 mM, 96% inhibition; 7 mM EDTA inhibits the enzyme by 96%
Castellaniella defragrans
N-ethylmaleimide
I80 of less than 0.5 mM
Castellaniella defragrans
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0033
-
geraniol
recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21C
Castellaniella defragrans
0.0044
-
(S)-perillyl alcohol
recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21C
Castellaniella defragrans
0.005
-
geraniol
pH 9.4, 21C
Castellaniella defragrans
0.006
-
cumic alcohol
recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21C
Castellaniella defragrans
0.007
-
(S)-perillyl alcohol
native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21C; pH 9.4, 21C
Castellaniella defragrans
0.021
-
cumic alcohol
native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21C
Castellaniella defragrans
0.021
-
p-cumic alcohol
pH 9.4, 21C
Castellaniella defragrans
0.0232
-
nerol
recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21C
Castellaniella defragrans
0.045
-
nerol
native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21C; pH 9.4, 21C
Castellaniella defragrans
0.05
-
geraniol
native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21C
Castellaniella defragrans
0.0575
-
citronellol
recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21C
Castellaniella defragrans
0.086
-
citronellol
native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21C; pH 9.4, 21C
Castellaniella defragrans
0.1157
-
benzyl alcohol
recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21C
Castellaniella defragrans
0.17
-
benzyl alcohol
native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21C; pH 9.4, 21C
Castellaniella defragrans
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Zn2+
contains zinc
Castellaniella defragrans
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
38272
-
2 * 38272, calculated; 2 * 38272, calculated from amino acid sequence
Castellaniella defragrans
39000
-
2 * 39000, SDS-PAGE
Castellaniella defragrans
52000
91000
gel filtration
Castellaniella defragrans
52000
-
gel filtration
Castellaniella defragrans
85000
-
PAGE
Castellaniella defragrans
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
geraniol + NAD+
Castellaniella defragrans
-
geranial + NADH + H+
-
-
?
geraniol + NAD+
Castellaniella defragrans 65Phen
-
geranial + NADH + H+
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Castellaniella defragrans
H1ZV38
-
-
Castellaniella defragrans 65Phen
H1ZV38
-
-
Purification (Commentary)
Purification (Commentary)
Organism
DEAE column chromatography and phenyl-Sepharose 6 gel filtration
Castellaniella defragrans
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
additional information
-
the crude cell extract shows a specific activity of 0.056 units/mg, while the 114fold purified enzyme shows a specific activity of 4.33 units/mg at pH 9.4 and 21C
Castellaniella defragrans
4.33
-
pH 9.4, 21C
Castellaniella defragrans
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(S)-perillyl alcohol + NAD+
most efficient substrate
718643
Castellaniella defragrans
? + NADH + H+
-
-
-
?
(S)-perillyl alcohol + NAD+
most efficient substrate
718643
Castellaniella defragrans 65Phen
? + NADH + H+
-
-
-
?
(S)-perillyl alcohol + NAD+
-
718643
Castellaniella defragrans
perillyl aldehyde + NADH
-
-
-
?
benzyl alcohol + NAD+
-
718643
Castellaniella defragrans
benzaldehyde + NADH + H+
-
-
-
?
benzyl alcohol + NAD+
-
718643
Castellaniella defragrans 65Phen
benzaldehyde + NADH + H+
-
-
-
?
benzyl alcohol + NAD+
-
718643
Castellaniella defragrans
?
-
-
-
?
citronellol + NAD+
-
718643
Castellaniella defragrans
?
-
-
-
?
citronellol + NAD+
-
718643
Castellaniella defragrans
citronellal + NADH + H+
-
-
-
?
cumic alcohol + NAD+
-
718643
Castellaniella defragrans
?
-
-
-
?
geraniol + dichlorophenolindophenol
-
718643
Castellaniella defragrans
?
-
-
-
?
geraniol + NAD+
-
718643
Castellaniella defragrans
geranial + NADH + H+
-
-
-
?
geraniol + NAD+
-
718643
Castellaniella defragrans 65Phen
geranial + NADH + H+
-
-
-
?
geraniol + oxidized dichlorophenolindophenol
-
718643
Castellaniella defragrans
geranial + reduced dichlorophenolindophenol
-
-
-
?
additional information
the enzyme cannot use phenazine methosulfate as alternative acceptor
718643
Castellaniella defragrans
?
-
-
-
-
additional information
phenazine methosulfate is not accepted as an electron acceptor
718643
Castellaniella defragrans
?
-
-
-
-
additional information
the enzyme cannot use phenazine methosulfate as alternative acceptor
718643
Castellaniella defragrans 65Phen
?
-
-
-
-
additional information
phenazine methosulfate is not accepted as an electron acceptor
718643
Castellaniella defragrans 65Phen
?
-
-
-
-
nerol + NAD+
-
718643
Castellaniella defragrans
?
-
-
-
?
nerol + NAD+
-
718643
Castellaniella defragrans
neral + NADH + H+
-
-
-
?
p-cumic alcohol + NAD+
-
718643
Castellaniella defragrans
? + NADH + H+
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 38272, calculated
Castellaniella defragrans
homodimer
2 * 38272, calculated from amino acid sequence; 2 * 39000, SDS-PAGE
Castellaniella defragrans
Synonyms
Synonyms
Commentary
Organism
GeDH
-
Castellaniella defragrans
geoA
-
Castellaniella defragrans
geraniol DH
-
Castellaniella defragrans
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.4
-
-
Castellaniella defragrans
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
NADPH is ineffective as a cosubstrate
Castellaniella defragrans
NAD+
dependent on, no activity with NADP+
Castellaniella defragrans
NADH
enzyme reduces one molecule of NAD+ to NADH per geraniol molecule provided
Castellaniella defragrans
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli strain BL21 Star(DE3); expression in Escherichia coli
Castellaniella defragrans
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
NADPH is ineffective as a cosubstrate
Castellaniella defragrans
NAD+
dependent on, no activity with NADP+
Castellaniella defragrans
NADH
enzyme reduces one molecule of NAD+ to NADH per geraniol molecule provided
Castellaniella defragrans
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1-cyclohexyl-N-(2-morpholinoethyl)carbodiimide
I80 of less than 34 mM
Castellaniella defragrans
diethylpyrocarbonate
I80 of less than 17 mM
Castellaniella defragrans
EDTA
7 mM, 96% inhibition; 7 mM EDTA inhibits the enzyme by 96%
Castellaniella defragrans
N-ethylmaleimide
I80 of less than 0.5 mM
Castellaniella defragrans
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0033
-
geraniol
recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21C
Castellaniella defragrans
0.0044
-
(S)-perillyl alcohol
recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21C
Castellaniella defragrans
0.005
-
geraniol
pH 9.4, 21C
Castellaniella defragrans
0.006
-
cumic alcohol
recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21C
Castellaniella defragrans
0.007
-
(S)-perillyl alcohol
native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21C; pH 9.4, 21C
Castellaniella defragrans
0.021
-
cumic alcohol
native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21C
Castellaniella defragrans
0.021
-
p-cumic alcohol
pH 9.4, 21C
Castellaniella defragrans
0.0232
-
nerol
recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21C
Castellaniella defragrans
0.045
-
nerol
native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21C; pH 9.4, 21C
Castellaniella defragrans
0.05
-
geraniol
native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21C
Castellaniella defragrans
0.0575
-
citronellol
recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21C
Castellaniella defragrans
0.086
-
citronellol
native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21C; pH 9.4, 21C
Castellaniella defragrans
0.1157
-
benzyl alcohol
recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21C
Castellaniella defragrans
0.17
-
benzyl alcohol
native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21C; pH 9.4, 21C
Castellaniella defragrans
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Zn2+
contains zinc
Castellaniella defragrans
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
38272
-
2 * 38272, calculated; 2 * 38272, calculated from amino acid sequence
Castellaniella defragrans
39000
-
2 * 39000, SDS-PAGE
Castellaniella defragrans
52000
91000
gel filtration
Castellaniella defragrans
52000
-
gel filtration
Castellaniella defragrans
85000
-
PAGE
Castellaniella defragrans
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
geraniol + NAD+
Castellaniella defragrans
-
geranial + NADH + H+
-
-
?
geraniol + NAD+
Castellaniella defragrans 65Phen
-
geranial + NADH + H+
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
DEAE column chromatography and phenyl-Sepharose 6 gel filtration
Castellaniella defragrans
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
additional information
-
the crude cell extract shows a specific activity of 0.056 units/mg, while the 114fold purified enzyme shows a specific activity of 4.33 units/mg at pH 9.4 and 21C
Castellaniella defragrans
4.33
-
pH 9.4, 21C
Castellaniella defragrans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(S)-perillyl alcohol + NAD+
most efficient substrate
718643
Castellaniella defragrans
? + NADH + H+
-
-
-
?
(S)-perillyl alcohol + NAD+
most efficient substrate
718643
Castellaniella defragrans 65Phen
? + NADH + H+
-
-
-
?
(S)-perillyl alcohol + NAD+
-
718643
Castellaniella defragrans
perillyl aldehyde + NADH
-
-
-
?
benzyl alcohol + NAD+
-
718643
Castellaniella defragrans
benzaldehyde + NADH + H+
-
-
-
?
benzyl alcohol + NAD+
-
718643
Castellaniella defragrans 65Phen
benzaldehyde + NADH + H+
-
-
-
?
benzyl alcohol + NAD+
-
718643
Castellaniella defragrans
?
-
-
-
?
citronellol + NAD+
-
718643
Castellaniella defragrans
?
-
-
-
?
citronellol + NAD+
-
718643
Castellaniella defragrans
citronellal + NADH + H+
-
-
-
?
cumic alcohol + NAD+
-
718643
Castellaniella defragrans
?
-
-
-
?
geraniol + dichlorophenolindophenol
-
718643
Castellaniella defragrans
?
-
-
-
?
geraniol + NAD+
-
718643
Castellaniella defragrans
geranial + NADH + H+
-
-
-
?
geraniol + NAD+
-
718643
Castellaniella defragrans 65Phen
geranial + NADH + H+
-
-
-
?
geraniol + oxidized dichlorophenolindophenol
-
718643
Castellaniella defragrans
geranial + reduced dichlorophenolindophenol
-
-
-
?
additional information
the enzyme cannot use phenazine methosulfate as alternative acceptor
718643
Castellaniella defragrans
?
-
-
-
-
additional information
phenazine methosulfate is not accepted as an electron acceptor
718643
Castellaniella defragrans
?
-
-
-
-
additional information
the enzyme cannot use phenazine methosulfate as alternative acceptor
718643
Castellaniella defragrans 65Phen
?
-
-
-
-
additional information
phenazine methosulfate is not accepted as an electron acceptor
718643
Castellaniella defragrans 65Phen
?
-
-
-
-
nerol + NAD+
-
718643
Castellaniella defragrans
?
-
-
-
?
nerol + NAD+
-
718643
Castellaniella defragrans
neral + NADH + H+
-
-
-
?
p-cumic alcohol + NAD+
-
718643
Castellaniella defragrans
? + NADH + H+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 38272, calculated
Castellaniella defragrans
homodimer
2 * 38272, calculated from amino acid sequence; 2 * 39000, SDS-PAGE
Castellaniella defragrans
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.4
-
-
Castellaniella defragrans
Expression
Organism
Commentary
Expression
Castellaniella defragrans
activitiy is induced during growth with limonene in comparison to growth with acetate; enzyme activity is induced during growth with limonene in comparison to growth with acetate
up
Expression (protein specific)
Organism
Commentary
Expression
Castellaniella defragrans
activitiy is induced during growth with limonene in comparison to growth with acetate; enzyme activity is induced during growth with limonene in comparison to growth with acetate
up
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
50
-
citronellol
recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21C
Castellaniella defragrans
100
-
citronellol
native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21C; pH 9.4, 21C
Castellaniella defragrans
110
-
benzyl alcohol
recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21C
Castellaniella defragrans
220
-
benzyl alcohol
native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21C; pH 9.4, 21C
Castellaniella defragrans
310
-
nerol
native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21C; pH 9.4, 21C; recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21C
Castellaniella defragrans
520
-
cumic alcohol
native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21C
Castellaniella defragrans
520
-
p-cumic alcohol
pH 9.4, 21C
Castellaniella defragrans
620
-
geraniol
recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21C
Castellaniella defragrans
940
-
cumic alcohol
recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21C
Castellaniella defragrans
1570
-
geraniol
native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21C; pH 9.4, 21C
Castellaniella defragrans
2020
-
(S)-perillyl alcohol
native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21C; pH 9.4, 21C
Castellaniella defragrans
3550
-
(S)-perillyl alcohol
recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21C
Castellaniella defragrans
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
50
-
citronellol
recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21C
Castellaniella defragrans
100
-
citronellol
native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21C; pH 9.4, 21C
Castellaniella defragrans
110
-
benzyl alcohol
recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21C
Castellaniella defragrans
220
-
benzyl alcohol
native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21C; pH 9.4, 21C
Castellaniella defragrans
310
-
nerol
native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21C; pH 9.4, 21C; recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21C
Castellaniella defragrans
520
-
cumic alcohol
native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21C
Castellaniella defragrans
520
-
p-cumic alcohol
pH 9.4, 21C
Castellaniella defragrans
620
-
geraniol
recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21C
Castellaniella defragrans
940
-
cumic alcohol
recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21C
Castellaniella defragrans
1570
-
geraniol
native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21C; pH 9.4, 21C
Castellaniella defragrans
2020
-
(S)-perillyl alcohol
native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21C; pH 9.4, 21C
Castellaniella defragrans
3550
-
(S)-perillyl alcohol
recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21C
Castellaniella defragrans
Other publictions for EC 1.1.1.347
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
718643
Lueddeke
Geraniol and geranial dehydrog ...
Castellaniella defragrans, Castellaniella defragrans 65Phen
Appl. Environ. Microbiol.
78
2128-2136
2012
-
-
1
-
-
-
4
14
-
1
5
2
-
2
-
-
1
-
-
-
2
-
20
2
3
-
-
-
-
1
-
-
3
-
-
-
-
-
1
3
-
-
-
-
4
-
14
-
1
5
2
-
-
-
1
-
-
2
-
20
2
-
-
-
-
1
-
-
-
1
-
-
1
12
12
686700
Noge
Geraniol dehydrogenase, the ke ...
Carpoglyphus lactis
FEBS J.
275
2807-2817
2008
-
-
-
-
-
-
-
2
-
-
3
1
-
1
-
-
1
-
-
-
2
-
5
1
1
1
-
-
1
1
1
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
-
3
1
-
-
-
1
-
-
2
-
5
1
1
-
-
1
1
1
-
-
-
1
1
-
1
1