Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetone | 40% (v/v), 40% activity | Thermoplasma acidophilum | |
ethanol | 40% (v/v), 30% activity | Thermoplasma acidophilum | |
methanol | 40% (v/v), 50% activity | Thermoplasma acidophilum | |
Urea | 40% (v/v), 85% activity | Thermoplasma acidophilum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
NAD+ | Km is superior to 30 mM. The Km value for NAD+ can not be defined precisely as it is impossible to reach saturation of the enzyme | Thermoplasma acidophilum | |
0.113 | - |
NADP+ | pH 7.0, 55°C | Thermoplasma acidophilum | |
10 | - |
beta-D-glucose | pH 7.0, 55°C | Thermoplasma acidophilum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
4 * 38000 | Thermoplasma acidophilum |
155000 | - |
gel filtration | Thermoplasma acidophilum |
Organic Solvent | Comment | Organism |
---|---|---|
Acetone | 50% (v/v), no appreciable loss of activity | Thermoplasma acidophilum |
Ethanol | 50% (v/v), no appreciable loss of activity | Thermoplasma acidophilum |
Methanol | 50% (v/v), no appreciable loss of activity | Thermoplasma acidophilum |
urea | 4 M, no appreciable loss of activity | Thermoplasma acidophilum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoplasma acidophilum | P13203 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermoplasma acidophilum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
320 | - |
pH 7.0, 55°C | Thermoplasma acidophilum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-D-glucose + NAD+ + H2O | - |
Thermoplasma acidophilum | D-gluconate + NADH + 2 H+ | - |
ir | |
beta-D-glucose + NADP+ + H2O | - |
Thermoplasma acidophilum | D-gluconate + NADPH + 2 H+ | - |
ir | |
D-galactose + NAD+ + H2O | - |
Thermoplasma acidophilum | D-galactonate + NADH + 2 H+ | - |
? | |
D-galactose + NADP+ + H2O | the activity is 70% of the activity with beta-D-glucose + NADP+ | Thermoplasma acidophilum | D-galactonate + NADPH + 2 H+ | - |
? | |
additional information | no activity with mannose or glucose 6-phosphate | Thermoplasma acidophilum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 38000 | Thermoplasma acidophilum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
assay at | Thermoplasma acidophilum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
catalytic activity is retained after 9 h | Thermoplasma acidophilum |
75 | - |
half-life: 3 h | Thermoplasma acidophilum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Thermoplasma acidophilum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | the enzyme is catalytically active with both NADP+ and NAD+. NADP+ and NAD+ bind at the same site on the enzyme | Thermoplasma acidophilum | |
NADP+ | the enzyme is catalytically active with both NADP+ and NAD+. NADP+ and NAD+ bind at the same site on the enzyme | Thermoplasma acidophilum |