Application | Comment | Organism |
---|---|---|
synthesis | the engineered enzyme mutant T173S can be used for increased production of poly(3-hydroxybutyrate) in a Corynebacterium glutamicum expression system | Cupriavidus necator |
Cloned (Comment) | Organism |
---|---|
gene phaB, recombinant expression of a PhaB mutant T173s enzyme from Ralstonia eutropha in Nicotiana tabacum cv. Bright Yellow-2 cells, the mutated PhaB increases the poly(3-hydroxybutyrate) (P(3HB)) content by threefold compared to control. Plasmids pRIphaC-phaB(WT) and pRIphaCphaB(TS) are introduced into BY-2 cells using the Agrobacterium-mediated transfection method. The PhaB-catalyzed reaction is suggested to be a rate-limiting step of P(3HB) biosynthesis in tobacco BY-2 cells | Cupriavidus necator |
Protein Variants | Comment | Organism |
---|---|---|
T173S | by random mutagenesis and high-throughput screening, enzyme mutant engineering for increased production of poly(3-hydroxybutyrate) in a Corynebacterium glutamicum expression system | Cupriavidus necator |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-3-hydroxyacyl-CoA + NADP+ | Cupriavidus necator | - |
3-oxoacyl-CoA + NADPH + H+ | - |
r | |
(R)-3-hydroxyacyl-CoA + NADP+ | Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | - |
3-oxoacyl-CoA + NADPH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cupriavidus necator | P14697 | - |
- |
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | P14697 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-3-hydroxyacyl-CoA + NADP+ | - |
Cupriavidus necator | 3-oxoacyl-CoA + NADPH + H+ | - |
r | |
(R)-3-hydroxyacyl-CoA + NADP+ | - |
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | 3-oxoacyl-CoA + NADPH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
NADPH-dependent acetoacetyl-CoA reductase | - |
Cupriavidus necator |
PhaB | - |
Cupriavidus necator |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Cupriavidus necator | |
NADPH | - |
Cupriavidus necator |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is rate-limiting in poly(3-hydroxybutyrate) biosynthesis. Poly(3-hydroxybutyrate) (P(3HB)) is synthesized from acetyl-CoA via three successive reactions. Two molecules of acetyl-CoA are condensed into acetoacetyl-CoA by a beta-ketothiolase (PhaA). Then NADPH-dependent acetoacetyl-CoA reductase (PhaB) converts acetoacetyl-CoA into (R)-3-hydroxybutyryl-CoA (3HB-CoA), which is finally polymerized into P(3HB) by polyhydroxyalkanoate synthase (PhaC) | Cupriavidus necator |