Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 Rosetta2 (DE3) pLysS cells and a fabG -temperature-sensitive mutant Escherichia coli CL104 strain | Mycolicibacterium smegmatis |
Crystallization (Comment) | Organism |
---|---|
apo and NADP+-bound enzyme forms, sitting drop vapor diffusion method, using 100 mM Na-HEPES (pH 7.5), 30% (v/v) PEG 400 and 200 mM magnesium chloride hexahydrate for the apo enzyme and 200 mM sodium citrate tribasic dehydrate and 20% (w/v) PEG 3350 for the NADP+-bound enzyme | Mycolicibacterium smegmatis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
100000 | - |
gel filtration | Mycolicibacterium smegmatis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetoacetyl-CoA + NADPH + H+ | Mycolicibacterium smegmatis | - |
(R)-3-hydroxybutyryl-CoA + NADP+ | - |
? | |
acetoacetyl-CoA + NADPH + H+ | Mycolicibacterium smegmatis mc2155 | - |
(R)-3-hydroxybutyryl-CoA + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycolicibacterium smegmatis | A0R723 | - |
- |
Mycolicibacterium smegmatis mc2155 | A0R723 | - |
- |
Purification (Comment) | Organism |
---|---|
nickel Sepharose bead chromatography | Mycolicibacterium smegmatis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.025 | - |
at pH 7.0 and 25°C | Mycolicibacterium smegmatis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetoacetyl-CoA + NADPH + H+ | - |
Mycolicibacterium smegmatis | (R)-3-hydroxybutyryl-CoA + NADP+ | - |
? | |
acetoacetyl-CoA + NADPH + H+ | - |
Mycolicibacterium smegmatis mc2155 | (R)-3-hydroxybutyryl-CoA + NADP+ | - |
? | |
additional information | no activity is detected when NADPH is replaced by NADH | Mycolicibacterium smegmatis | ? | - |
- |
|
additional information | no activity is detected when NADPH is replaced by NADH | Mycolicibacterium smegmatis mc2155 | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 25000, SDS-PAGE | Mycolicibacterium smegmatis |
Synonyms | Comment | Organism |
---|---|---|
beta-ketoacyl reductase | - |
Mycolicibacterium smegmatis |
ketoacyl reductase | - |
Mycolicibacterium smegmatis |
MSMEG_6753 | - |
Mycolicibacterium smegmatis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Mycolicibacterium smegmatis |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is dispensable for mycobacterial growth | Mycolicibacterium smegmatis |