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Literature summary for 1.1.1.372 extracted from

  • Liepins, J.; Kuorelahti, S.; Penttilae, M.; Richard, P.
    Enzymes for the NADPH-dependent reduction of dihydroxyacetone and D-glyceraldehyde and L-glyceraldehyde in the mould Hypocrea jecorina (2006), FEBS J., 273, 4229-4235.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Saccharomyces cerevisiae Trichoderma reesei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
methylglyoxal at pH 7.0 and 30°C Trichoderma reesei
0.9
-
D-glyceraldehyde at pH 7.0 and 30°C Trichoderma reesei
0.9
-
diacetyl at pH 7.0 and 30°C Trichoderma reesei
0.9
-
L-glyceraldehyde at pH 7.0 and 30°C Trichoderma reesei
2.4
-
Glyoxal at pH 7.0 and 30°C Trichoderma reesei
5.8
-
dihydroxyacetone at pH 7.0 and 30°C Trichoderma reesei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36232
-
x * 36232, calculated from amino acid sequence Trichoderma reesei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glyceraldehyde + NADPH + H+ Trichoderma reesei
-
glycerol + NADP+
-
?
D-glyceraldehyde + NADPH + H+ Trichoderma reesei RUT C-30
-
glycerol + NADP+
-
?
L-glyceraldehyde + NADPH + H+ Trichoderma reesei the activity with L-glyceraldehyde is only slightly lower compared to D-glyceraldehyde glycerol + NADP+
-
?
L-glyceraldehyde + NADPH + H+ Trichoderma reesei RUT C-30 the activity with L-glyceraldehyde is only slightly lower compared to D-glyceraldehyde glycerol + NADP+
-
?
additional information Trichoderma reesei no activity with NADH or glycerol. Very much reduced activity with dihydroxyacetone. No activity with C5 or C6 sugars (acetoin, D-ribose, D-xylose, and D-glucose) ?
-
?
additional information Trichoderma reesei RUT C-30 no activity with NADH or glycerol. Very much reduced activity with dihydroxyacetone. No activity with C5 or C6 sugars (acetoin, D-ribose, D-xylose, and D-glucose) ?
-
?

Organism

Organism UniProt Comment Textmining
Trichoderma reesei Q0GYU5 synonym Trichoderma reesei
-
Trichoderma reesei RUT C-30 Q0GYU5 synonym Trichoderma reesei
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Trichoderma reesei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glyceraldehyde + NADPH + H+
-
Trichoderma reesei glycerol + NADP+
-
?
D-glyceraldehyde + NADPH + H+
-
Trichoderma reesei RUT C-30 glycerol + NADP+
-
?
diacetyl + NADPH + H+
-
Trichoderma reesei ?
-
?
dihydroxyacetone + NADPH + H+
-
Trichoderma reesei ?
-
?
glyoxal + NADPH + H+
-
Trichoderma reesei ?
-
?
glyoxal + NADPH + H+
-
Trichoderma reesei RUT C-30 ?
-
?
L-glyceraldehyde + NADPH + H+ the activity with L-glyceraldehyde is only slightly lower compared to D-glyceraldehyde Trichoderma reesei glycerol + NADP+
-
?
L-glyceraldehyde + NADPH + H+ the activity with L-glyceraldehyde is only slightly lower compared to D-glyceraldehyde Trichoderma reesei RUT C-30 glycerol + NADP+
-
?
methylglyoxal + NADPH + H+
-
Trichoderma reesei ?
-
?
methylglyoxal + NADPH + H+
-
Trichoderma reesei RUT C-30 ?
-
?
additional information no activity with NADH or glycerol. Very much reduced activity with dihydroxyacetone. No activity with C5 or C6 sugars (acetoin, D-ribose, D-xylose, and D-glucose) Trichoderma reesei ?
-
?
additional information no activity with NADH or glycerol. Very much reduced activity with dihydroxyacetone. No activity with C5 or C6 sugars (acetoin, D-ribose, D-xylose, and D-glucose) Trichoderma reesei RUT C-30 ?
-
?

Subunits

Subunits Comment Organism
? x * 36232, calculated from amino acid sequence Trichoderma reesei

Synonyms

Synonyms Comment Organism
gld1
-
Trichoderma reesei
NADP-dependent glycerol dehydrogenase
-
Trichoderma reesei

Cofactor

Cofactor Comment Organism Structure
NADPH specific cofactor Trichoderma reesei

General Information

General Information Comment Organism
metabolism the enzyme is active in pathways for sugar acid catabolism such as D-galacturonate catabolism Trichoderma reesei