Cloned (Comment) | Organism |
---|---|
expressed in Saccharomyces cerevisiae | Trichoderma reesei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
methylglyoxal | at pH 7.0 and 30°C | Trichoderma reesei | |
0.9 | - |
D-glyceraldehyde | at pH 7.0 and 30°C | Trichoderma reesei | |
0.9 | - |
diacetyl | at pH 7.0 and 30°C | Trichoderma reesei | |
0.9 | - |
L-glyceraldehyde | at pH 7.0 and 30°C | Trichoderma reesei | |
2.4 | - |
Glyoxal | at pH 7.0 and 30°C | Trichoderma reesei | |
5.8 | - |
dihydroxyacetone | at pH 7.0 and 30°C | Trichoderma reesei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36232 | - |
x * 36232, calculated from amino acid sequence | Trichoderma reesei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde + NADPH + H+ | Trichoderma reesei | - |
glycerol + NADP+ | - |
? | |
D-glyceraldehyde + NADPH + H+ | Trichoderma reesei RUT C-30 | - |
glycerol + NADP+ | - |
? | |
L-glyceraldehyde + NADPH + H+ | Trichoderma reesei | the activity with L-glyceraldehyde is only slightly lower compared to D-glyceraldehyde | glycerol + NADP+ | - |
? | |
L-glyceraldehyde + NADPH + H+ | Trichoderma reesei RUT C-30 | the activity with L-glyceraldehyde is only slightly lower compared to D-glyceraldehyde | glycerol + NADP+ | - |
? | |
additional information | Trichoderma reesei | no activity with NADH or glycerol. Very much reduced activity with dihydroxyacetone. No activity with C5 or C6 sugars (acetoin, D-ribose, D-xylose, and D-glucose) | ? | - |
? | |
additional information | Trichoderma reesei RUT C-30 | no activity with NADH or glycerol. Very much reduced activity with dihydroxyacetone. No activity with C5 or C6 sugars (acetoin, D-ribose, D-xylose, and D-glucose) | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trichoderma reesei | Q0GYU5 | synonym Trichoderma reesei | - |
Trichoderma reesei RUT C-30 | Q0GYU5 | synonym Trichoderma reesei | - |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Trichoderma reesei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde + NADPH + H+ | - |
Trichoderma reesei | glycerol + NADP+ | - |
? | |
D-glyceraldehyde + NADPH + H+ | - |
Trichoderma reesei RUT C-30 | glycerol + NADP+ | - |
? | |
diacetyl + NADPH + H+ | - |
Trichoderma reesei | ? | - |
? | |
dihydroxyacetone + NADPH + H+ | - |
Trichoderma reesei | ? | - |
? | |
glyoxal + NADPH + H+ | - |
Trichoderma reesei | ? | - |
? | |
glyoxal + NADPH + H+ | - |
Trichoderma reesei RUT C-30 | ? | - |
? | |
L-glyceraldehyde + NADPH + H+ | the activity with L-glyceraldehyde is only slightly lower compared to D-glyceraldehyde | Trichoderma reesei | glycerol + NADP+ | - |
? | |
L-glyceraldehyde + NADPH + H+ | the activity with L-glyceraldehyde is only slightly lower compared to D-glyceraldehyde | Trichoderma reesei RUT C-30 | glycerol + NADP+ | - |
? | |
methylglyoxal + NADPH + H+ | - |
Trichoderma reesei | ? | - |
? | |
methylglyoxal + NADPH + H+ | - |
Trichoderma reesei RUT C-30 | ? | - |
? | |
additional information | no activity with NADH or glycerol. Very much reduced activity with dihydroxyacetone. No activity with C5 or C6 sugars (acetoin, D-ribose, D-xylose, and D-glucose) | Trichoderma reesei | ? | - |
? | |
additional information | no activity with NADH or glycerol. Very much reduced activity with dihydroxyacetone. No activity with C5 or C6 sugars (acetoin, D-ribose, D-xylose, and D-glucose) | Trichoderma reesei RUT C-30 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 36232, calculated from amino acid sequence | Trichoderma reesei |
Synonyms | Comment | Organism |
---|---|---|
gld1 | - |
Trichoderma reesei |
NADP-dependent glycerol dehydrogenase | - |
Trichoderma reesei |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | specific cofactor | Trichoderma reesei |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is active in pathways for sugar acid catabolism such as D-galacturonate catabolism | Trichoderma reesei |