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Literature summary for 1.1.1.4 extracted from

  • Gonzalez, E.; Fernandez, M.R.; Larroy, C.; Sola, L.; Pericas, M.A.; Pares, X.; Biosca, J.A.
    Characterization of a (2R,3R)-2,3-butaendiol dehydrogenase as the Saccharomyces cerevisiae YAL060W gene product (2000), J. Biol. Chem., 275, 35876-35885.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Saccharomyces cerevisiae Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
2 * 41000, SDS-PAGE Saccharomyces cerevisiae
81800
-
gel filtration Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [Āµmol/min/mg] Specific Activity Maximum [Āµmol/min/mg] Comment Organism
968
-
-
Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2R,3R)-2,3-butanediol + NAD+
-
Saccharomyces cerevisiae (3R)-acetoin + NADH
-
r
(3R,3S)-acetoin + NADH
-
Saccharomyces cerevisiae (2R,3R)-2,3-butanediol + meso-2,3-butanediol + NAD+
-
r
2,3-pentanediol + NAD+
-
Saccharomyces cerevisiae 4-hydroxy-3-pentanone + 3-hydroxy-2-pentanone + NADH
-
?
meso-2,3-butanediol + NAD+
-
Saccharomyces cerevisiae D-(-)-acetoin + NADH (3S)-acetoin r
additional information 3S)-2,3-butanediol, (3R/3S)-acetoin, glycerol, sorbitol or xylitol are no substrates Saccharomyces cerevisiae ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 41000, SDS-PAGE Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
acetoin reduction Saccharomyces cerevisiae
8
-
substrate oxidation Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
NAD+ no activity with NADP+ as cofactor Saccharomyces cerevisiae