KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
acetone | pH 7.5, 25°C | Clostridium autoethanogenum | |
1.2 | - |
Butanone | pH 7.5, 25°C | Clostridium autoethanogenum | |
5.5 | - |
acetaldehyde | pH 7.5, 25°C | Clostridium autoethanogenum | |
65 | - |
(2R,3R)-2,3-butanediol | pH 7.5, 25°C | Clostridium autoethanogenum | |
71 | - |
acetoin | pH 7.5, 25°C | Clostridium autoethanogenum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium autoethanogenum | F8TEL7 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R,3R)-2,3-butanediol + NADP+ | no detectable activity with (2S,3S)-2,3-butanediol | Clostridium autoethanogenum | (R)-acetoin + NADPH + H+ | - |
r | |
acetaldehyde + NADPH + H+ | - |
Clostridium autoethanogenum | ethanol + NADP+ | - |
? | |
acetoin + 2 NADPH + 2 H+ | - |
Clostridium autoethanogenum | (2R,3R)-2,3-butanediol + meso-2,3-butanediol + 2 NADP+ | - |
r | |
acetone + NADPH + H+ | - |
Clostridium autoethanogenum | propan-2-ol + NADP+ | - |
? | |
butanone + NADPH + H+ | - |
Clostridium autoethanogenum | butan-2-ol + NADP+ | - |
? | |
additional information | enzyme is a strictly NADPH-dependent primary-secondary alcohol dehydrogenase able to reduce acetoin to 2,3-butanediol. The enzyme accepts a range of 2-, 3-, and 4-carbon substrates, including the nonphysiological ketones acetone and butanone | Clostridium autoethanogenum | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.1 | - |
(2R,3R)-2,3-butanediol | pH 7.5, 25°C | Clostridium autoethanogenum | |
44 | - |
Butanone | pH 7.5, 25°C | Clostridium autoethanogenum | |
51.4 | - |
acetone | pH 7.5, 25°C | Clostridium autoethanogenum | |
93 | - |
acetaldehyde | pH 7.5, 25°C | Clostridium autoethanogenum | |
145 | - |
acetoin | pH 7.5, 25°C | Clostridium autoethanogenum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no activity with NADH | Clostridium autoethanogenum | |
NADPH | - |
Clostridium autoethanogenum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.033 | - |
(2R,3R)-2,3-butanediol | pH 7.5, 25°C | Clostridium autoethanogenum | |
2 | - |
acetoin | pH 7.5, 25°C | Clostridium autoethanogenum | |
17 | - |
acetaldehyde | pH 7.5, 25°C | Clostridium autoethanogenum | |
38 | - |
Butanone | pH 7.5, 25°C | Clostridium autoethanogenum | |
86 | - |
acetone | pH 7.5, 25°C | Clostridium autoethanogenum |