BRENDA - Enzyme Database
show all sequences of 1.1.1.4

Reconstruction of an acetogenic 2,3-butanediol pathway involving a novel NADPH-dependent primary-secondary alcohol dehydrogenase

Koepke, M.; Gerth, M.L.; Maddock, D.J.; Mueller, A.P.; Liew, F.; Simpson, S.D.; Patrick, W.M.; Appl. Environ. Microbiol. 80, 3394-3403 (2014) View publication on PubMedView publication on EuropePMC

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.6
-
acetone
pH 7.5, 25°C
Clostridium autoethanogenum
1.2
-
Butanone
pH 7.5, 25°C
Clostridium autoethanogenum
5.5
-
acetaldehyde
pH 7.5, 25°C
Clostridium autoethanogenum
65
-
(2R,3R)-2,3-butanediol
pH 7.5, 25°C
Clostridium autoethanogenum
71
-
acetoin
pH 7.5, 25°C
Clostridium autoethanogenum
Organism
Organism
UniProt
Commentary
Textmining
Clostridium autoethanogenum
F8TEL7
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(2R,3R)-2,3-butanediol + NADP+
no detectable activity with (2S,3S)-2,3-butanediol
737501
Clostridium autoethanogenum
(R)-acetoin + NADPH + H+
-
-
-
r
acetaldehyde + NADPH + H+
-
737501
Clostridium autoethanogenum
ethanol + NADP+
-
-
-
?
acetoin + 2 NADPH + 2 H+
-
737501
Clostridium autoethanogenum
(2R,3R)-2,3-butanediol + meso-2,3-butanediol + 2 NADP+
-
-
-
r
acetone + NADPH + H+
-
737501
Clostridium autoethanogenum
propan-2-ol + NADP+
-
-
-
?
butanone + NADPH + H+
-
737501
Clostridium autoethanogenum
butan-2-ol + NADP+
-
-
-
?
additional information
enzyme is a strictly NADPH-dependent primary-secondary alcohol dehydrogenase able to reduce acetoin to 2,3-butanediol. The enzyme accepts a range of 2-, 3-, and 4-carbon substrates, including the nonphysiological ketones acetone and butanone
737501
Clostridium autoethanogenum
?
-
-
-
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.1
-
(2R,3R)-2,3-butanediol
pH 7.5, 25°C
Clostridium autoethanogenum
44
-
Butanone
pH 7.5, 25°C
Clostridium autoethanogenum
51.4
-
acetone
pH 7.5, 25°C
Clostridium autoethanogenum
93
-
acetaldehyde
pH 7.5, 25°C
Clostridium autoethanogenum
145
-
acetoin
pH 7.5, 25°C
Clostridium autoethanogenum
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
no activity with NADH
Clostridium autoethanogenum
NADPH
-
Clostridium autoethanogenum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
no activity with NADH
Clostridium autoethanogenum
NADPH
-
Clostridium autoethanogenum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.6
-
acetone
pH 7.5, 25°C
Clostridium autoethanogenum
1.2
-
Butanone
pH 7.5, 25°C
Clostridium autoethanogenum
5.5
-
acetaldehyde
pH 7.5, 25°C
Clostridium autoethanogenum
65
-
(2R,3R)-2,3-butanediol
pH 7.5, 25°C
Clostridium autoethanogenum
71
-
acetoin
pH 7.5, 25°C
Clostridium autoethanogenum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(2R,3R)-2,3-butanediol + NADP+
no detectable activity with (2S,3S)-2,3-butanediol
737501
Clostridium autoethanogenum
(R)-acetoin + NADPH + H+
-
-
-
r
acetaldehyde + NADPH + H+
-
737501
Clostridium autoethanogenum
ethanol + NADP+
-
-
-
?
acetoin + 2 NADPH + 2 H+
-
737501
Clostridium autoethanogenum
(2R,3R)-2,3-butanediol + meso-2,3-butanediol + 2 NADP+
-
-
-
r
acetone + NADPH + H+
-
737501
Clostridium autoethanogenum
propan-2-ol + NADP+
-
-
-
?
butanone + NADPH + H+
-
737501
Clostridium autoethanogenum
butan-2-ol + NADP+
-
-
-
?
additional information
enzyme is a strictly NADPH-dependent primary-secondary alcohol dehydrogenase able to reduce acetoin to 2,3-butanediol. The enzyme accepts a range of 2-, 3-, and 4-carbon substrates, including the nonphysiological ketones acetone and butanone
737501
Clostridium autoethanogenum
?
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.1
-
(2R,3R)-2,3-butanediol
pH 7.5, 25°C
Clostridium autoethanogenum
44
-
Butanone
pH 7.5, 25°C
Clostridium autoethanogenum
51.4
-
acetone
pH 7.5, 25°C
Clostridium autoethanogenum
93
-
acetaldehyde
pH 7.5, 25°C
Clostridium autoethanogenum
145
-
acetoin
pH 7.5, 25°C
Clostridium autoethanogenum
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.033
-
(2R,3R)-2,3-butanediol
pH 7.5, 25°C
Clostridium autoethanogenum
2
-
acetoin
pH 7.5, 25°C
Clostridium autoethanogenum
17
-
acetaldehyde
pH 7.5, 25°C
Clostridium autoethanogenum
38
-
Butanone
pH 7.5, 25°C
Clostridium autoethanogenum
86
-
acetone
pH 7.5, 25°C
Clostridium autoethanogenum
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.033
-
(2R,3R)-2,3-butanediol
pH 7.5, 25°C
Clostridium autoethanogenum
2
-
acetoin
pH 7.5, 25°C
Clostridium autoethanogenum
17
-
acetaldehyde
pH 7.5, 25°C
Clostridium autoethanogenum
38
-
Butanone
pH 7.5, 25°C
Clostridium autoethanogenum
86
-
acetone
pH 7.5, 25°C
Clostridium autoethanogenum
Other publictions for EC 1.1.1.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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-
-
2
-
-
-
-
8
-
2
-
4
-
6
-
-
2
-
-
-
17
-
31
-
8
2
-
-
8
2
-
2
6
-
-
-
-
-
2
6
-
-
-
-
-
-
8
-
2
-
4
-
-
-
2
-
-
17
-
31
-
2
-
-
8
2
-
2
-
-
2
2
-
8
8
760761
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no activity in Serratia marcescens
Biotechnol. Biofuels
13
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-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
761810
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-
-
-
-
1
-
-
-
-
-
-
2
-
4
-
-
-
-
-
-
-
-
4
-
2
1
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
762382
Muschallik
-
Synthesis of alpha-hydroxy ke ...
Alkalihalobacillus clausii, Alkalihalobacillus clausii DSM 8716T
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10
12206-12216
2020
-
-
1
-
1
-
-
-
-
1
-
2
-
2
-
-
1
-
-
-
-
-
8
-
5
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
2
-
-
-
1
-
-
-
-
8
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
760463
Gong
-
Cloning, expression and chara ...
Bacillus thuringiensis serovar kurstaki, Bacillus thuringiensis serovar kurstaki ACCC 10066
Biocatal. Agricult. Biotechnol.
22
10137
2019
1
-
1
-
-
-
2
2
-
1
-
4
-
2
-
-
1
-
-
-
-
1
8
1
3
2
-
1
-
2
-
1
2
-
1
-
1
-
1
2
-
-
-
-
2
-
2
-
1
-
4
-
-
-
1
-
-
-
1
8
1
2
-
1
-
2
-
1
1
-
1
1
-
-
-
761834
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Synthetic engineering of Cory ...
Bacillus subtilis subsp. subtilis, Bacillus subtilis subsp. subtilis 168
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18
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-
-
1
-
1
-
-
-
-
-
-
2
-
5
-
-
-
-
-
-
-
-
2
-
2
1
-
-
-
1
-
-
2
-
-
-
-
-
1
2
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
756608
Liu
2,3-Butanediol catabolism in ...
Pseudomonas aeruginosa, Pseudomonas aeruginosa ATCC 15692, Pseudomonas aeruginosa 1C, Pseudomonas aeruginosa PRS 101, Pseudomonas aeruginosa DSM 22644, Pseudomonas aeruginosa CIP 104116, Pseudomonas aeruginosa LMG 12228, Pseudomonas aeruginosa JCM 14847
Environ. Microbiol.
20
3927-3940
2018
-
-
1
-
-
-
-
1
-
1
-
16
-
11
-
-
-
-
-
-
8
-
48
-
5
1
-
-
-
1
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
1
-
1
-
16
-
-
-
-
-
-
8
-
48
-
1
-
-
-
1
-
-
-
-
2
2
-
-
-
760411
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Paenibacillus brasilensis, Paenibacillus brasilensis PB24
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102
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2018
-
1
1
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
4
-
4
-
-
-
-
-
-
-
1
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
740952
Liang
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Escherichia coli, Escherichia coli BW25113
Metab. Eng.
39
181-191
2017
-
-
-
-
-
-
-
2
-
-
-
-
-
32
-
-
-
-
-
-
-
-
4
-
-
-
-
-
2
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
2
-
-
-
-
-
1
1
-
2
2
760716
Yamada
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Bacillus amyloliquefaciens, Bacillus amyloliquefaciens UCMB5033
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245
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2017
-
-
1
-
1
-
-
-
-
-
-
2
-
3
-
-
-
-
-
1
-
-
2
-
4
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
761565
Cui
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-
1
1
-
1
-
-
2
-
-
-
2
1
3
-
-
1
-
-
-
-
-
6
-
2
1
-
1
-
1
-
1
2
-
-
-
-
1
1
2
-
1
-
-
-
-
2
-
-
-
2
1
-
-
1
-
-
-
-
6
-
1
-
1
-
1
-
1
-
-
-
-
-
-
-
761646
Li
Reducing diacetyl production ...
Saccharomyces uvarum, Saccharomyces uvarum WY1
J. Ind. Microbiol. Biotechnol.
44
1541-1550
2017
-
-
1
-
1
-
-
-
-
-
-
2
-
5
-
-
-
-
-
-
-
-
2
-
3
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
2
2
1
-
-
762259
Zhang
Effect of deletion of 2,3-but ...
Bacillus subtilis, Bacillus subtilis BS168D
Prep. Biochem. Biotechnol.
47
761-767
2017
1
-
1
-
1
-
-
-
-
-
-
2
-
5
-
-
-
-
-
1
-
-
2
-
3
-
-
-
-
-
-
-
2
-
-
-
1
-
1
2
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
737512
Rados
Stereospecificity of Corynebac ...
Corynebacterium glutamicum, Corynebacterium glutamicum ATCC 13032
Appl. Microbiol. Biotechnol.
100
10573-10583
2016
-
-
1
-
-
-
-
4
-
-
-
4
-
37
-
-
1
-
-
-
-
-
10
-
3
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
4
-
-
-
4
-
-
-
1
-
-
-
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
4
4
737519
de Oliveira
Synthetic operon for (R,R)-2,3 ...
Bacillus subtilis, Bacillus subtilis WN1038
Appl. Microbiol. Biotechnol.
100
719-728
2016
-
-
1
-
-
-
-
-
-
-
2
2
-
8
-
-
-
-
-
-
-
-
2
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
738345
Ge
-
Contracted but effective: Prod ...
Bacillus licheniformis, Bacillus licheniformis MW3
Green Chem.
18
4693-4703
2016
-
-
1
-
-
-
-
-
-
-
-
4
-
3
-
-
1
-
-
-
14
-
20
-
3
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
1
-
-
14
-
20
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
739694
Bae
Efficient production of acetoi ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae BY4741
Sci. Rep.
6
27667
2016
-
-
-
-
-
-
-
-
-
-
-
-
-
76
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
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-
-
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-
-
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-
-
-
-
-
-
737847
Kang
Enhanced production of 2,3-but ...
Bacillus sp. (in: Bacteria), Bacillus sp. (in: Bacteria) BRC1
Bioprocess Biosyst. Eng.
38
299-305
2015
-
-
1
-
-
-
-
4
-
1
-
2
-
2
-
-
1
-
-
-
-
-
14
-
2
1
-
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2
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1
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1
1
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1
-
-
-
-
14
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
738800
Bai
Engineered Serratia marcescens ...
Bacillus subtilis, Serratia marcescens, Bacillus subtilis 168, Serratia marcescens MG1
J. Ind. Microbiol. Biotechnol.
42
779-786
2015
-
-
2
-
-
-
-
-
-
-
2
8
-
110
-
-
2
-
-
-
-
-
12
2
4
-
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-
4
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8
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-
-
12
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
738953
Zhao
Identification and characteriz ...
Corynebacterium crenatum, Corynebacterium crenatum SYPA5-5
Lett. Appl. Microbiol.
61
573-579
2015
-
-
1
-
-
-
4
-
-
5
1
-
-
11
-
-
1
-
-
-
-
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1
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-
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-
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1
-
1
-
2
-
-
-
-
-
-
-
-
-
739127
Yu
Characterization of a (2R,3R)- ...
Rhodococcus erythropolis, Rhodococcus erythropolis WZ010
Molecules
20
7156-7173
2015
1
-
1
-
-
-
15
4
-
3
2
3
-
10
-
-
1
-
-
-
-
-
19
2
2
2
-
1
4
2
1
-
2
-
-
-
1
-
1
2
-
-
-
-
15
-
4
-
3
2
3
-
-
-
1
-
-
-
-
19
2
2
-
1
4
2
1
-
-
-
-
-
-
4
4
739571
Maddock
Substitutions at the cofactor ...
Clostridium autoethanogenum
Protein Eng. Des. Sel.
28
251-258
2015
-
-
1
-
7
-
-
21
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
21
-
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2
-
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-
7
-
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21
-
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-
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-
-
3
-
-
-
-
21
-
-
-
-
-
-
-
-
21
21
737501
Koepke
Reconstruction of an acetogeni ...
Clostridium autoethanogenum
Appl. Environ. Microbiol.
80
3394-3403
2014
-
-
-
-
-
-
-
5
-
-
-
-
-
8
-
-
-
-
-
-
-
-
6
-
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-
-
5
-
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2
-
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2
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5
-
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-
-
-
-
-
6
-
-
-
-
5
-
-
-
-
-
-
-
-
5
5
738472
Cui
Utilization of excess NADH in ...
Bacillus licheniformis
J. Appl. Microbiol.
117
690-698
2014
-
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
2
-
1
-
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2
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
738637
Wang
Glycerol dehydrogenase plays a ...
Klebsiella pneumoniae, Klebsiella pneumoniae ATCC 25955
J. Biol. Chem.
289
6080-6090
2014
-
-
1
-
-
-
-
8
-
-
1
2
-
2
-
-
1
-
-
-
-
-
10
1
2
-
-
-
8
-
-
-
1
-
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-
-
-
1
1
-
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-
-
8
-
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1
2
-
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-
1
-
-
-
-
10
1
-
-
-
8
-
-
-
-
1
-
-
1
8
8
738878
Kochius
-
Enantioselective enzymatic syn ...
Haloarcula marismortui
J. Mol. Catal. B
103
61-66
2014
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
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-
-
-
-
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1
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-
1
1
-
-
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-
-
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-
-
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-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
738880
Hao
-
Biochemical characterization o ...
Bacillus subtilis, Bacillus subtilis 168
J. Mol. Catal. B
109
184-190
2014
-
-
-
-
1
-
-
-
-
-
-
4
-
100
-
-
-
-
-
-
-
-
4
-
2
-
-
-
-
-
-
-
1
-
-
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-
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-
1
-
1
-
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-
-
-
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-
4
-
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-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
740827
Zhang
Cloning, expression and charac ...
Serratia marcescens, Serratia marcescens H30
J. Ind. Microbiol. Biotechnol.
41
1319-1327
2014
-
-
1
-
-
-
4
-
-
5
1
-
-
6
-
-
-
-
-
-
2
-
10
1
4
1
1
1
-
2
1
-
2
-
-
-
-
-
1
2
-
-
-
-
4
-
-
-
5
1
-
-
-
-
-
-
-
2
-
10
1
1
1
1
-
2
1
-
-
-
1
1
-
-
-
722559
Gao
A 2,3-butanediol dehydrogenase ...
Paenibacillus polymyxa, Paenibacillus polymyxa ZJ-9
J. Basic Microbiol.
53
733-741
2013
-
-
1
-
-
-
-
6
-
-
2
-
-
7
-
-
1
-
-
1
1
-
4
1
1
1
-
-
-
2
-
-
3
-
-
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-
-
1
3
-
-
-
-
-
-
6
-
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2
-
-
-
-
1
-
1
1
-
4
1
1
-
-
-
2
-
-
-
-
-
-
-
-
-
737366
Miao
Crystallization and preliminar ...
Bacillus coagulans, Bacillus coagulans 2-6
Acta Crystallogr. Sect. F
69
1140-1142
2013
-
-
1
1
-
-
-
-
-
1
2
2
-
10
-
-
1
-
-
-
-
-
2
2
2
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
1
2
2
-
-
-
1
-
-
-
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
721345
Yu
Novel (2R,3R)-2,3-butanediol d ...
Paenibacillus polymyxa, Paenibacillus polymyxa ATCC 12321
Appl. Environ. Microbiol.
77
4230-4233
2011
-
-
1
-
-
-
-
5
-
-
-
2
-
7
-
-
-
-
-
-
-
-
14
-
2
1
-
-
-
2
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
5
-
-
-
2
-
-
-
-
-
-
-
-
14
-
1
-
-
-
2
-
-
-
-
1
1
-
-
-
721872
Takeda
Identification and characteriz ...
Mycolicibacterium smegmatis, Mycolicibacterium vanbaalenii, Mycolicibacterium gilvum, Mycobacterium sp., Mycobacterium sp. B-009
Biosci. Biotechnol. Biochem.
75
2384-2389
2011
-
-
4
-
-
-
5
1
-
-
-
4
-
12
-
-
1
-
-
-
-
-
14
1
8
1
-
1
-
2
-
1
2
-
1
-
-
-
4
2
-
-
-
-
5
-
1
-
-
-
4
-
-
-
1
-
-
-
-
14
1
1
-
1
-
2
-
1
1
-
4
4
-
-
-
710944
Gonzalez
Role of Saccharomyces cerevisi ...
Saccharomyces cerevisiae
Appl. Environ. Microbiol.
76
670-679
2010
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
1
1
1
-
-
695776
Ehsani
Engineering of 2,3-butanediol ...
Saccharomyces cerevisiae
Appl. Environ. Microbiol.
75
3196-3205
2009
-
-
-
-
-
-
1
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
696883
Ehsani
Reversal of coenzyme specifici ...
Saccharomyces cerevisiae
Biotechnol. Bioeng.
104
381-389
2009
-
-
-
-
3
-
-
9
-
-
-
-
-
9
-
-
-
-
-
-
8
-
2
-
3
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
3
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
8
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
685764
Ji
Enhanced 2,3-butanediol produc ...
Klebsiella oxytoca, Klebsiella oxytoca ME-303
Biotechnol. Lett.
30
731-734
2008
-
1
-
-
1
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
695754
Nicholson
The Bacillus subtilis ydjL (bd ...
Bacillus subtilis
Appl. Environ. Microbiol.
74
6832-6838
2008
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
685903
Cabral
Citrate metabolism by Enteroco ...
Enterococcus faecium, Enterococcus durans, Enterococcus durans Ov 421, Enterococcus faecium ET C9
Can. J. Microbiol.
53
607-615
2007
-
-
-
-
-
-
-
-
-
-
-
4
-
10
-
-
-
-
-
2
2
-
8
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
2
2
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
667266
Machielsen
Production and characterizatio ...
Pyrococcus furiosus
Appl. Environ. Microbiol.
72
233-238
2006
-
-
1
-
-
-
-
4
-
-
2
-
-
1
-
-
1
-
-
-
-
-
7
1
-
1
-
1
-
2
-
-
3
-
-
-
-
-
1
3
-
-
-
-
-
-
4
-
-
2
-
-
-
-
1
-
-
-
-
7
1
1
-
1
-
2
-
-
-
-
-
-
-
-
-
655087
Marwoto
-
Enhancement of (R,R)-2,3-butan ...
Paenibacillus polymyxa
Biotechnol. Lett.
24
109-114
2002
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
246409
Gonzalez
Characterization and functiona ...
Saccharomyces cerevisiae
Chem. Biol. Interact.
130
425-434
2001
-
-
1
-
-
-
1
5
-
-
-
-
-
4
-
-
1
-
-
-
-
-
3
-
-
-
-
-
-
1
1
-
1
-
-
-
-
-
1
1
-
-
-
-
1
-
5
-
-
-
-
-
-
-
1
-
-
-
-
3
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
246413
Gonzalez
Characterization of a (2R,3R)- ...
Saccharomyces cerevisiae
J. Biol. Chem.
275
35876-35885
2000
-
-
1
-
-
-
-
-
-
-
2
-
-
4
-
-
1
-
-
-
1
-
5
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
1
-
5
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
246412
Carballo
Purification, characterization ...
Klebsiella aerogenes
Eur. J. Biochem.
198
327-332
1991
-
-
-
-
-
-
-
8
-
-
2
-
-
1
-
-
1
-
-
-
1
-
7
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
8
-
-
2
-
-
-
-
1
-
-
1
-
7
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
246410
Heidlas
Purification and characterizat ...
Saccharomyces cerevisiae
Arch. Microbiol.
154
267-273
1990
-
-
-
1
-
-
3
-
-
2
2
-
-
6
-
-
-
-
-
-
-
-
11
1
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
-
-
-
3
-
-
-
2
2
-
-
-
-
-
-
-
-
-
11
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
246411
Crow
Properties of 2,3-butanediol d ...
Lactococcus lactis
Appl. Environ. Microbiol.
56
1656-1665
1990
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
2
-
-
-
4
-
3
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
4
-
3
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
246406
Nokhal
-
Fermentation enzymes in strain ...
Paracoccus denitrificans
Arch. Microbiol.
145
197-201
1986
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
246404
Sadaharu
-
Stereospecific and electrophor ...
Bacillus sp. (in: Bacteria), Brevibacillus sp., Enterobacter sp., Micrococcus sp., Pseudomonas sp.
J. Ferment. Technol.
61
467-471
1983
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-
-
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-
-
-
-
-
-
-
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5
-
-
-
-
-
-
-
-
10
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
10
-
-
-
-
-
-
-
-
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-
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-
-
-
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246407
Hhn-Bentz
Bacterial 2,3-butanediol dehyd ...
Gluconobacter oxydans, Aeromonas hydrophila, Bacillus subtilis, Paenibacillus polymyxa
Arch. Microbiol.
116
197-203
1978
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-
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1
7
-
4
1
-
-
4
-
-
1
-
-
-
2
1
11
-
-
2
-
1
-
2
-
1
8
-
-
-
-
-
-
8
-
-
-
-
1
-
7
-
4
1
-
-
-
-
1
-
-
2
1
11
-
2
-
1
-
2
-
1
-
-
-
-
-
-
-