BRENDA - Enzyme Database
show all sequences of 1.1.1.4

2,3-Butanediol catabolism in Pseudomonas aeruginosa PAO1

Liu, Q.; Liu, Y.; Kang, Z.; Xiao, D.; Gao, C.; Xu, P.; Ma, C.; Environ. Microbiol. 20, 3927-3940 (2018) View publication on PubMed

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
genetic structure of the 2,3-BD utilization operon in Pseudomonas aeruginosa strain PAO1 and comparisons, overview
Pseudomonas aeruginosa
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.057
-
(2R,3R)-butane-2,3-diol
pH 7.4, 30°C
Pseudomonas aeruginosa
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Zn2+
zinc binding enzyme
Pseudomonas aeruginosa
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
(2R,3S)-butane-2,3-diol + NAD+
Pseudomonas aeruginosa
-
(3R)-acetoin + NADH + H+
-
-
?
(2R,3S)-butane-2,3-diol + NAD+
Pseudomonas aeruginosa ATCC 15692
-
(3R)-acetoin + NADH + H+
-
-
?
(2R,3S)-butane-2,3-diol + NAD+
Pseudomonas aeruginosa 1C
-
(3R)-acetoin + NADH + H+
-
-
?
(2R,3S)-butane-2,3-diol + NAD+
Pseudomonas aeruginosa PRS 101
-
(3R)-acetoin + NADH + H+
-
-
?
(2R,3S)-butane-2,3-diol + NAD+
Pseudomonas aeruginosa DSM 22644
-
(3R)-acetoin + NADH + H+
-
-
?
(2R,3S)-butane-2,3-diol + NAD+
Pseudomonas aeruginosa CIP 104116
-
(3R)-acetoin + NADH + H+
-
-
?
(2R,3S)-butane-2,3-diol + NAD+
Pseudomonas aeruginosa LMG 12228
-
(3R)-acetoin + NADH + H+
-
-
?
(2R,3S)-butane-2,3-diol + NAD+
Pseudomonas aeruginosa JCM 14847
-
(3R)-acetoin + NADH + H+
-
-
?
(R,R)-butane-2,3-diol + NAD+
Pseudomonas aeruginosa
-
(R)-acetoin + NADH + H+
-
-
r
(R,R)-butane-2,3-diol + NAD+
Pseudomonas aeruginosa ATCC 15692
-
(R)-acetoin + NADH + H+
-
-
r
(R,R)-butane-2,3-diol + NAD+
Pseudomonas aeruginosa 1C
-
(R)-acetoin + NADH + H+
-
-
r
(R,R)-butane-2,3-diol + NAD+
Pseudomonas aeruginosa PRS 101
-
(R)-acetoin + NADH + H+
-
-
r
(R,R)-butane-2,3-diol + NAD+
Pseudomonas aeruginosa DSM 22644
-
(R)-acetoin + NADH + H+
-
-
r
(R,R)-butane-2,3-diol + NAD+
Pseudomonas aeruginosa CIP 104116
-
(R)-acetoin + NADH + H+
-
-
r
(R,R)-butane-2,3-diol + NAD+
Pseudomonas aeruginosa LMG 12228
-
(R)-acetoin + NADH + H+
-
-
r
(R,R)-butane-2,3-diol + NAD+
Pseudomonas aeruginosa JCM 14847
-
(R)-acetoin + NADH + H+
-
-
r
Organism
Organism
UniProt
Commentary
Textmining
Pseudomonas aeruginosa
Q9HWM8
-
-
Pseudomonas aeruginosa 1C
Q9HWM8
-
-
Pseudomonas aeruginosa ATCC 15692
Q9HWM8
-
-
Pseudomonas aeruginosa CIP 104116
Q9HWM8
-
-
Pseudomonas aeruginosa DSM 22644
Q9HWM8
-
-
Pseudomonas aeruginosa JCM 14847
Q9HWM8
-
-
Pseudomonas aeruginosa LMG 12228
Q9HWM8
-
-
Pseudomonas aeruginosa PRS 101
Q9HWM8
-
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.155
-
pH 7.4, 30°C, substrate propane-1,2-diol
Pseudomonas aeruginosa
0.331
-
pH 7.4, 30°C, substrate 1-hydroxy-2-butanone
Pseudomonas aeruginosa
0.383
-
pH 7.4, 30°C, substrate butane-1,2-diol
Pseudomonas aeruginosa
1.179
-
pH 7.4, 30°C, substrate (2R,3S)-butane-2,3-diol
Pseudomonas aeruginosa
1.346
-
pH 7.4, 30°C, substrate 2,3-pentanedione
Pseudomonas aeruginosa
1.538
-
pH 7.4, 30°C, substrate (2R,3R)-butane-2,3-diol
Pseudomonas aeruginosa
1.949
-
pH 7.4, 30°C, substrate rac acetoin
Pseudomonas aeruginosa
2.154
-
pH 7.4, 30°C, substrate diacetyl
Pseudomonas aeruginosa
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(2R,3S)-butane-2,3-diol + NAD+
-
756608
Pseudomonas aeruginosa
(3R)-acetoin + NADH + H+
-
-
-
?
(2R,3S)-butane-2,3-diol + NAD+
stereoselective interconversion
756608
Pseudomonas aeruginosa
(3R)-acetoin + NADH + H+
-
-
-
?
(2R,3S)-butane-2,3-diol + NAD+
-
756608
Pseudomonas aeruginosa ATCC 15692
(3R)-acetoin + NADH + H+
-
-
-
?
(2R,3S)-butane-2,3-diol + NAD+
stereoselective interconversion
756608
Pseudomonas aeruginosa ATCC 15692
(3R)-acetoin + NADH + H+
-
-
-
?
(2R,3S)-butane-2,3-diol + NAD+
-
756608
Pseudomonas aeruginosa 1C
(3R)-acetoin + NADH + H+
-
-
-
?
(2R,3S)-butane-2,3-diol + NAD+
stereoselective interconversion
756608
Pseudomonas aeruginosa 1C
(3R)-acetoin + NADH + H+
-
-
-
?
(2R,3S)-butane-2,3-diol + NAD+
-
756608
Pseudomonas aeruginosa PRS 101
(3R)-acetoin + NADH + H+
-
-
-
?
(2R,3S)-butane-2,3-diol + NAD+
stereoselective interconversion
756608
Pseudomonas aeruginosa PRS 101
(3R)-acetoin + NADH + H+
-
-
-
?
(2R,3S)-butane-2,3-diol + NAD+
-
756608
Pseudomonas aeruginosa DSM 22644
(3R)-acetoin + NADH + H+
-
-
-
?
(2R,3S)-butane-2,3-diol + NAD+
stereoselective interconversion
756608
Pseudomonas aeruginosa DSM 22644
(3R)-acetoin + NADH + H+
-
-
-
?
(2R,3S)-butane-2,3-diol + NAD+
-
756608
Pseudomonas aeruginosa CIP 104116
(3R)-acetoin + NADH + H+
-
-
-
?
(2R,3S)-butane-2,3-diol + NAD+
stereoselective interconversion
756608
Pseudomonas aeruginosa CIP 104116
(3R)-acetoin + NADH + H+
-
-
-
?
(2R,3S)-butane-2,3-diol + NAD+
-
756608
Pseudomonas aeruginosa LMG 12228
(3R)-acetoin + NADH + H+
-
-
-
?
(2R,3S)-butane-2,3-diol + NAD+
stereoselective interconversion
756608
Pseudomonas aeruginosa LMG 12228
(3R)-acetoin + NADH + H+
-
-
-
?
(2R,3S)-butane-2,3-diol + NAD+
-
756608
Pseudomonas aeruginosa JCM 14847
(3R)-acetoin + NADH + H+
-
-
-
?
(2R,3S)-butane-2,3-diol + NAD+
stereoselective interconversion
756608
Pseudomonas aeruginosa JCM 14847
(3R)-acetoin + NADH + H+
-
-
-
?
(R,R)-butane-2,3-diol + NAD+
-
756608
Pseudomonas aeruginosa
(R)-acetoin + NADH + H+
-
-
-
r
(R,R)-butane-2,3-diol + NAD+
-
756608
Pseudomonas aeruginosa ATCC 15692
(R)-acetoin + NADH + H+
-
-
-
r
(R,R)-butane-2,3-diol + NAD+
-
756608
Pseudomonas aeruginosa 1C
(R)-acetoin + NADH + H+
-
-
-
r
(R,R)-butane-2,3-diol + NAD+
-
756608
Pseudomonas aeruginosa PRS 101
(R)-acetoin + NADH + H+
-
-
-
r
(R,R)-butane-2,3-diol + NAD+
-
756608
Pseudomonas aeruginosa DSM 22644
(R)-acetoin + NADH + H+
-
-
-
r
(R,R)-butane-2,3-diol + NAD+
-
756608
Pseudomonas aeruginosa CIP 104116
(R)-acetoin + NADH + H+
-
-
-
r
(R,R)-butane-2,3-diol + NAD+
-
756608
Pseudomonas aeruginosa LMG 12228
(R)-acetoin + NADH + H+
-
-
-
r
(R,R)-butane-2,3-diol + NAD+
-
756608
Pseudomonas aeruginosa JCM 14847
(R)-acetoin + NADH + H+
-
-
-
r
(R,R)-butane-2,3-diol + NAD+
stereoselective interconversion
756608
Pseudomonas aeruginosa
(3R)-acetoin + NADH + H+
-
-
-
r
(R,R)-butane-2,3-diol + NAD+
stereoselective interconversion
756608
Pseudomonas aeruginosa ATCC 15692
(3R)-acetoin + NADH + H+
-
-
-
r
(R,R)-butane-2,3-diol + NAD+
stereoselective interconversion
756608
Pseudomonas aeruginosa 1C
(3R)-acetoin + NADH + H+
-
-
-
r
(R,R)-butane-2,3-diol + NAD+
stereoselective interconversion
756608
Pseudomonas aeruginosa PRS 101
(3R)-acetoin + NADH + H+
-
-
-
r
(R,R)-butane-2,3-diol + NAD+
stereoselective interconversion
756608
Pseudomonas aeruginosa DSM 22644
(3R)-acetoin + NADH + H+
-
-
-
r
(R,R)-butane-2,3-diol + NAD+
stereoselective interconversion
756608
Pseudomonas aeruginosa CIP 104116
(3R)-acetoin + NADH + H+
-
-
-
r
(R,R)-butane-2,3-diol + NAD+
stereoselective interconversion
756608
Pseudomonas aeruginosa LMG 12228
(3R)-acetoin + NADH + H+
-
-
-
r
(R,R)-butane-2,3-diol + NAD+
stereoselective interconversion
756608
Pseudomonas aeruginosa JCM 14847
(3R)-acetoin + NADH + H+
-
-
-
r
1-hydroxy-2-butanone + NADH + H+
-
756608
Pseudomonas aeruginosa
? + NAD+
-
-
-
?
2,3-pentandione + NADH + H+
-
756608
Pseudomonas aeruginosa
? + NAD+
-
-
-
?
butane-1,2-diol + NAD+
-
756608
Pseudomonas aeruginosa
? + NADH + H+
-
-
-
?
diacetyl + NADH + H+
-
756608
Pseudomonas aeruginosa
(2R,3R)-butane-2,3-diol + NAD+
-
-
-
r
diacetyl + NADH + H+
-
756608
Pseudomonas aeruginosa
(3R)-acetoin + NAD+
-
-
-
r
additional information
no activity with (2S,3S)-2,3-butane-2,3-diol, but the (2R,3R)-2,3-butanediol dehydrogenase is also active with meso-2,3-butanediol. No activity with 2-butanol, 1,3-butanediol, 1,2-pentanediol, 1,3-propanediol, and glycerol in the oxidation reaction. And no activity with 2,4-pentanedione, butanone, 2,5-hexanedione, n-butanal and 1,3-dihydroxypropanone in the reduction reaction. Substrate specificity, overview. (2R,3R)-2,3-BDH reduces diacetyl into (3R)-acetoin and (2R,3R)-2,3-BD, while racemic acetoin is reduced into (2R,3R)-2,3-BD and meso-2,3-BD
756608
Pseudomonas aeruginosa
?
-
-
-
-
additional information
no activity with (2S,3S)-2,3-butane-2,3-diol, but the (2R,3R)-2,3-butanediol dehydrogenase is also active with meso-2,3-butanediol. No activity with 2-butanol, 1,3-butanediol, 1,2-pentanediol, 1,3-propanediol, and glycerol in the oxidation reaction. And no activity with 2,4-pentanedione, butanone, 2,5-hexanedione, n-butanal and 1,3-dihydroxypropanone in the reduction reaction. Substrate specificity, overview. (2R,3R)-2,3-BDH reduces diacetyl into (3R)-acetoin and (2R,3R)-2,3-BD, while racemic acetoin is reduced into (2R,3R)-2,3-BD and meso-2,3-BD
756608
Pseudomonas aeruginosa ATCC 15692
?
-
-
-
-
additional information
no activity with (2S,3S)-2,3-butane-2,3-diol, but the (2R,3R)-2,3-butanediol dehydrogenase is also active with meso-2,3-butanediol. No activity with 2-butanol, 1,3-butanediol, 1,2-pentanediol, 1,3-propanediol, and glycerol in the oxidation reaction. And no activity with 2,4-pentanedione, butanone, 2,5-hexanedione, n-butanal and 1,3-dihydroxypropanone in the reduction reaction. Substrate specificity, overview. (2R,3R)-2,3-BDH reduces diacetyl into (3R)-acetoin and (2R,3R)-2,3-BD, while racemic acetoin is reduced into (2R,3R)-2,3-BD and meso-2,3-BD
756608
Pseudomonas aeruginosa 1C
?
-
-
-
-
additional information
no activity with (2S,3S)-2,3-butane-2,3-diol, but the (2R,3R)-2,3-butanediol dehydrogenase is also active with meso-2,3-butanediol. No activity with 2-butanol, 1,3-butanediol, 1,2-pentanediol, 1,3-propanediol, and glycerol in the oxidation reaction. And no activity with 2,4-pentanedione, butanone, 2,5-hexanedione, n-butanal and 1,3-dihydroxypropanone in the reduction reaction. Substrate specificity, overview. (2R,3R)-2,3-BDH reduces diacetyl into (3R)-acetoin and (2R,3R)-2,3-BD, while racemic acetoin is reduced into (2R,3R)-2,3-BD and meso-2,3-BD
756608
Pseudomonas aeruginosa PRS 101
?
-
-
-
-
additional information
no activity with (2S,3S)-2,3-butane-2,3-diol, but the (2R,3R)-2,3-butanediol dehydrogenase is also active with meso-2,3-butanediol. No activity with 2-butanol, 1,3-butanediol, 1,2-pentanediol, 1,3-propanediol, and glycerol in the oxidation reaction. And no activity with 2,4-pentanedione, butanone, 2,5-hexanedione, n-butanal and 1,3-dihydroxypropanone in the reduction reaction. Substrate specificity, overview. (2R,3R)-2,3-BDH reduces diacetyl into (3R)-acetoin and (2R,3R)-2,3-BD, while racemic acetoin is reduced into (2R,3R)-2,3-BD and meso-2,3-BD
756608
Pseudomonas aeruginosa DSM 22644
?
-
-
-
-
additional information
no activity with (2S,3S)-2,3-butane-2,3-diol, but the (2R,3R)-2,3-butanediol dehydrogenase is also active with meso-2,3-butanediol. No activity with 2-butanol, 1,3-butanediol, 1,2-pentanediol, 1,3-propanediol, and glycerol in the oxidation reaction. And no activity with 2,4-pentanedione, butanone, 2,5-hexanedione, n-butanal and 1,3-dihydroxypropanone in the reduction reaction. Substrate specificity, overview. (2R,3R)-2,3-BDH reduces diacetyl into (3R)-acetoin and (2R,3R)-2,3-BD, while racemic acetoin is reduced into (2R,3R)-2,3-BD and meso-2,3-BD
756608
Pseudomonas aeruginosa CIP 104116
?
-
-
-
-
additional information
no activity with (2S,3S)-2,3-butane-2,3-diol, but the (2R,3R)-2,3-butanediol dehydrogenase is also active with meso-2,3-butanediol. No activity with 2-butanol, 1,3-butanediol, 1,2-pentanediol, 1,3-propanediol, and glycerol in the oxidation reaction. And no activity with 2,4-pentanedione, butanone, 2,5-hexanedione, n-butanal and 1,3-dihydroxypropanone in the reduction reaction. Substrate specificity, overview. (2R,3R)-2,3-BDH reduces diacetyl into (3R)-acetoin and (2R,3R)-2,3-BD, while racemic acetoin is reduced into (2R,3R)-2,3-BD and meso-2,3-BD
756608
Pseudomonas aeruginosa LMG 12228
?
-
-
-
-
additional information
no activity with (2S,3S)-2,3-butane-2,3-diol, but the (2R,3R)-2,3-butanediol dehydrogenase is also active with meso-2,3-butanediol. No activity with 2-butanol, 1,3-butanediol, 1,2-pentanediol, 1,3-propanediol, and glycerol in the oxidation reaction. And no activity with 2,4-pentanedione, butanone, 2,5-hexanedione, n-butanal and 1,3-dihydroxypropanone in the reduction reaction. Substrate specificity, overview. (2R,3R)-2,3-BDH reduces diacetyl into (3R)-acetoin and (2R,3R)-2,3-BD, while racemic acetoin is reduced into (2R,3R)-2,3-BD and meso-2,3-BD
756608
Pseudomonas aeruginosa JCM 14847
?
-
-
-
-
propane-1,2-diol + NAD+
-
756608
Pseudomonas aeruginosa
? + NADH + H+
-
-
-
?
rac acetoin + NADH + H+
-
756608
Pseudomonas aeruginosa
(2R,3R)-butane-2,3-diol + NAD+
-
-
-
?
rac acetoin + NADH + H+
-
756608
Pseudomonas aeruginosa
(2R,3S)-butane-2,3-diol + NAD+
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
(2R,3R)-2,3-BDH
-
Pseudomonas aeruginosa
(2R,3R)-2,3-butanediol dehydrogenase
-
Pseudomonas aeruginosa
BDH
-
Pseudomonas aeruginosa
More
cf. EC 1.1.1.303
Pseudomonas aeruginosa
PA4153
locus name
Pseudomonas aeruginosa
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Pseudomonas aeruginosa
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
assay at
Pseudomonas aeruginosa
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Pseudomonas aeruginosa
NADH
-
Pseudomonas aeruginosa
Cloned(Commentary) (protein specific)
Commentary
Organism
genetic structure of the 2,3-BD utilization operon in Pseudomonas aeruginosa strain PAO1 and comparisons, overview
Pseudomonas aeruginosa
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Pseudomonas aeruginosa
NADH
-
Pseudomonas aeruginosa
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.057
-
(2R,3R)-butane-2,3-diol
pH 7.4, 30°C
Pseudomonas aeruginosa
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Zn2+
zinc binding enzyme
Pseudomonas aeruginosa
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
(2R,3S)-butane-2,3-diol + NAD+
Pseudomonas aeruginosa
-
(3R)-acetoin + NADH + H+
-
-
?
(2R,3S)-butane-2,3-diol + NAD+
Pseudomonas aeruginosa ATCC 15692
-
(3R)-acetoin + NADH + H+
-
-
?
(2R,3S)-butane-2,3-diol + NAD+
Pseudomonas aeruginosa 1C
-
(3R)-acetoin + NADH + H+
-
-
?
(2R,3S)-butane-2,3-diol + NAD+
Pseudomonas aeruginosa PRS 101
-
(3R)-acetoin + NADH + H+
-
-
?
(2R,3S)-butane-2,3-diol + NAD+
Pseudomonas aeruginosa DSM 22644
-
(3R)-acetoin + NADH + H+
-
-
?
(2R,3S)-butane-2,3-diol + NAD+
Pseudomonas aeruginosa CIP 104116
-
(3R)-acetoin + NADH + H+
-
-
?
(2R,3S)-butane-2,3-diol + NAD+
Pseudomonas aeruginosa LMG 12228
-
(3R)-acetoin + NADH + H+
-
-
?
(2R,3S)-butane-2,3-diol + NAD+
Pseudomonas aeruginosa JCM 14847
-
(3R)-acetoin + NADH + H+
-
-
?
(R,R)-butane-2,3-diol + NAD+
Pseudomonas aeruginosa
-
(R)-acetoin + NADH + H+
-
-
r
(R,R)-butane-2,3-diol + NAD+
Pseudomonas aeruginosa ATCC 15692
-
(R)-acetoin + NADH + H+
-
-
r
(R,R)-butane-2,3-diol + NAD+
Pseudomonas aeruginosa 1C
-
(R)-acetoin + NADH + H+
-
-
r
(R,R)-butane-2,3-diol + NAD+
Pseudomonas aeruginosa PRS 101
-
(R)-acetoin + NADH + H+
-
-
r
(R,R)-butane-2,3-diol + NAD+
Pseudomonas aeruginosa DSM 22644
-
(R)-acetoin + NADH + H+
-
-
r
(R,R)-butane-2,3-diol + NAD+
Pseudomonas aeruginosa CIP 104116
-
(R)-acetoin + NADH + H+
-
-
r
(R,R)-butane-2,3-diol + NAD+
Pseudomonas aeruginosa LMG 12228
-
(R)-acetoin + NADH + H+
-
-
r
(R,R)-butane-2,3-diol + NAD+
Pseudomonas aeruginosa JCM 14847
-
(R)-acetoin + NADH + H+
-
-
r
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.155
-
pH 7.4, 30°C, substrate propane-1,2-diol
Pseudomonas aeruginosa
0.331
-
pH 7.4, 30°C, substrate 1-hydroxy-2-butanone
Pseudomonas aeruginosa
0.383
-
pH 7.4, 30°C, substrate butane-1,2-diol
Pseudomonas aeruginosa
1.179
-
pH 7.4, 30°C, substrate (2R,3S)-butane-2,3-diol
Pseudomonas aeruginosa
1.346
-
pH 7.4, 30°C, substrate 2,3-pentanedione
Pseudomonas aeruginosa
1.538
-
pH 7.4, 30°C, substrate (2R,3R)-butane-2,3-diol
Pseudomonas aeruginosa
1.949
-
pH 7.4, 30°C, substrate rac acetoin
Pseudomonas aeruginosa
2.154
-
pH 7.4, 30°C, substrate diacetyl
Pseudomonas aeruginosa
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(2R,3S)-butane-2,3-diol + NAD+
-
756608
Pseudomonas aeruginosa
(3R)-acetoin + NADH + H+
-
-
-
?
(2R,3S)-butane-2,3-diol + NAD+
stereoselective interconversion
756608
Pseudomonas aeruginosa
(3R)-acetoin + NADH + H+
-
-
-
?
(2R,3S)-butane-2,3-diol + NAD+
-
756608
Pseudomonas aeruginosa ATCC 15692
(3R)-acetoin + NADH + H+
-
-
-
?
(2R,3S)-butane-2,3-diol + NAD+
stereoselective interconversion
756608
Pseudomonas aeruginosa ATCC 15692
(3R)-acetoin + NADH + H+
-
-
-
?
(2R,3S)-butane-2,3-diol + NAD+
-
756608
Pseudomonas aeruginosa 1C
(3R)-acetoin + NADH + H+
-
-
-
?
(2R,3S)-butane-2,3-diol + NAD+
stereoselective interconversion
756608
Pseudomonas aeruginosa 1C
(3R)-acetoin + NADH + H+
-
-
-
?
(2R,3S)-butane-2,3-diol + NAD+
-
756608
Pseudomonas aeruginosa PRS 101
(3R)-acetoin + NADH + H+
-
-
-
?
(2R,3S)-butane-2,3-diol + NAD+
stereoselective interconversion
756608
Pseudomonas aeruginosa PRS 101
(3R)-acetoin + NADH + H+
-
-
-
?
(2R,3S)-butane-2,3-diol + NAD+
-
756608
Pseudomonas aeruginosa DSM 22644
(3R)-acetoin + NADH + H+
-
-
-
?
(2R,3S)-butane-2,3-diol + NAD+
stereoselective interconversion
756608
Pseudomonas aeruginosa DSM 22644
(3R)-acetoin + NADH + H+
-
-
-
?
(2R,3S)-butane-2,3-diol + NAD+
-
756608
Pseudomonas aeruginosa CIP 104116
(3R)-acetoin + NADH + H+
-
-
-
?
(2R,3S)-butane-2,3-diol + NAD+
stereoselective interconversion
756608
Pseudomonas aeruginosa CIP 104116
(3R)-acetoin + NADH + H+
-
-
-
?
(2R,3S)-butane-2,3-diol + NAD+
-
756608
Pseudomonas aeruginosa LMG 12228
(3R)-acetoin + NADH + H+
-
-
-
?
(2R,3S)-butane-2,3-diol + NAD+
stereoselective interconversion
756608
Pseudomonas aeruginosa LMG 12228
(3R)-acetoin + NADH + H+
-
-
-
?
(2R,3S)-butane-2,3-diol + NAD+
-
756608
Pseudomonas aeruginosa JCM 14847
(3R)-acetoin + NADH + H+
-
-
-
?
(2R,3S)-butane-2,3-diol + NAD+
stereoselective interconversion
756608
Pseudomonas aeruginosa JCM 14847
(3R)-acetoin + NADH + H+
-
-
-
?
(R,R)-butane-2,3-diol + NAD+
-
756608
Pseudomonas aeruginosa
(R)-acetoin + NADH + H+
-
-
-
r
(R,R)-butane-2,3-diol + NAD+
-
756608
Pseudomonas aeruginosa ATCC 15692
(R)-acetoin + NADH + H+
-
-
-
r
(R,R)-butane-2,3-diol + NAD+
-
756608
Pseudomonas aeruginosa 1C
(R)-acetoin + NADH + H+
-
-
-
r
(R,R)-butane-2,3-diol + NAD+
-
756608
Pseudomonas aeruginosa PRS 101
(R)-acetoin + NADH + H+
-
-
-
r
(R,R)-butane-2,3-diol + NAD+
-
756608
Pseudomonas aeruginosa DSM 22644
(R)-acetoin + NADH + H+
-
-
-
r
(R,R)-butane-2,3-diol + NAD+
-
756608
Pseudomonas aeruginosa CIP 104116
(R)-acetoin + NADH + H+
-
-
-
r
(R,R)-butane-2,3-diol + NAD+
-
756608
Pseudomonas aeruginosa LMG 12228
(R)-acetoin + NADH + H+
-
-
-
r
(R,R)-butane-2,3-diol + NAD+
-
756608
Pseudomonas aeruginosa JCM 14847
(R)-acetoin + NADH + H+
-
-
-
r
(R,R)-butane-2,3-diol + NAD+
stereoselective interconversion
756608
Pseudomonas aeruginosa
(3R)-acetoin + NADH + H+
-
-
-
r
(R,R)-butane-2,3-diol + NAD+
stereoselective interconversion
756608
Pseudomonas aeruginosa ATCC 15692
(3R)-acetoin + NADH + H+
-
-
-
r
(R,R)-butane-2,3-diol + NAD+
stereoselective interconversion
756608
Pseudomonas aeruginosa 1C
(3R)-acetoin + NADH + H+
-
-
-
r
(R,R)-butane-2,3-diol + NAD+
stereoselective interconversion
756608
Pseudomonas aeruginosa PRS 101
(3R)-acetoin + NADH + H+
-
-
-
r
(R,R)-butane-2,3-diol + NAD+
stereoselective interconversion
756608
Pseudomonas aeruginosa DSM 22644
(3R)-acetoin + NADH + H+
-
-
-
r
(R,R)-butane-2,3-diol + NAD+
stereoselective interconversion
756608
Pseudomonas aeruginosa CIP 104116
(3R)-acetoin + NADH + H+
-
-
-
r
(R,R)-butane-2,3-diol + NAD+
stereoselective interconversion
756608
Pseudomonas aeruginosa LMG 12228
(3R)-acetoin + NADH + H+
-
-
-
r
(R,R)-butane-2,3-diol + NAD+
stereoselective interconversion
756608
Pseudomonas aeruginosa JCM 14847
(3R)-acetoin + NADH + H+
-
-
-
r
1-hydroxy-2-butanone + NADH + H+
-
756608
Pseudomonas aeruginosa
? + NAD+
-
-
-
?
2,3-pentandione + NADH + H+
-
756608
Pseudomonas aeruginosa
? + NAD+
-
-
-
?
butane-1,2-diol + NAD+
-
756608
Pseudomonas aeruginosa
? + NADH + H+
-
-
-
?
diacetyl + NADH + H+
-
756608
Pseudomonas aeruginosa
(2R,3R)-butane-2,3-diol + NAD+
-
-
-
r
diacetyl + NADH + H+
-
756608
Pseudomonas aeruginosa
(3R)-acetoin + NAD+
-
-
-
r
additional information
no activity with (2S,3S)-2,3-butane-2,3-diol, but the (2R,3R)-2,3-butanediol dehydrogenase is also active with meso-2,3-butanediol. No activity with 2-butanol, 1,3-butanediol, 1,2-pentanediol, 1,3-propanediol, and glycerol in the oxidation reaction. And no activity with 2,4-pentanedione, butanone, 2,5-hexanedione, n-butanal and 1,3-dihydroxypropanone in the reduction reaction. Substrate specificity, overview. (2R,3R)-2,3-BDH reduces diacetyl into (3R)-acetoin and (2R,3R)-2,3-BD, while racemic acetoin is reduced into (2R,3R)-2,3-BD and meso-2,3-BD
756608
Pseudomonas aeruginosa
?
-
-
-
-
additional information
no activity with (2S,3S)-2,3-butane-2,3-diol, but the (2R,3R)-2,3-butanediol dehydrogenase is also active with meso-2,3-butanediol. No activity with 2-butanol, 1,3-butanediol, 1,2-pentanediol, 1,3-propanediol, and glycerol in the oxidation reaction. And no activity with 2,4-pentanedione, butanone, 2,5-hexanedione, n-butanal and 1,3-dihydroxypropanone in the reduction reaction. Substrate specificity, overview. (2R,3R)-2,3-BDH reduces diacetyl into (3R)-acetoin and (2R,3R)-2,3-BD, while racemic acetoin is reduced into (2R,3R)-2,3-BD and meso-2,3-BD
756608
Pseudomonas aeruginosa ATCC 15692
?
-
-
-
-
additional information
no activity with (2S,3S)-2,3-butane-2,3-diol, but the (2R,3R)-2,3-butanediol dehydrogenase is also active with meso-2,3-butanediol. No activity with 2-butanol, 1,3-butanediol, 1,2-pentanediol, 1,3-propanediol, and glycerol in the oxidation reaction. And no activity with 2,4-pentanedione, butanone, 2,5-hexanedione, n-butanal and 1,3-dihydroxypropanone in the reduction reaction. Substrate specificity, overview. (2R,3R)-2,3-BDH reduces diacetyl into (3R)-acetoin and (2R,3R)-2,3-BD, while racemic acetoin is reduced into (2R,3R)-2,3-BD and meso-2,3-BD
756608
Pseudomonas aeruginosa 1C
?
-
-
-
-
additional information
no activity with (2S,3S)-2,3-butane-2,3-diol, but the (2R,3R)-2,3-butanediol dehydrogenase is also active with meso-2,3-butanediol. No activity with 2-butanol, 1,3-butanediol, 1,2-pentanediol, 1,3-propanediol, and glycerol in the oxidation reaction. And no activity with 2,4-pentanedione, butanone, 2,5-hexanedione, n-butanal and 1,3-dihydroxypropanone in the reduction reaction. Substrate specificity, overview. (2R,3R)-2,3-BDH reduces diacetyl into (3R)-acetoin and (2R,3R)-2,3-BD, while racemic acetoin is reduced into (2R,3R)-2,3-BD and meso-2,3-BD
756608
Pseudomonas aeruginosa PRS 101
?
-
-
-
-
additional information
no activity with (2S,3S)-2,3-butane-2,3-diol, but the (2R,3R)-2,3-butanediol dehydrogenase is also active with meso-2,3-butanediol. No activity with 2-butanol, 1,3-butanediol, 1,2-pentanediol, 1,3-propanediol, and glycerol in the oxidation reaction. And no activity with 2,4-pentanedione, butanone, 2,5-hexanedione, n-butanal and 1,3-dihydroxypropanone in the reduction reaction. Substrate specificity, overview. (2R,3R)-2,3-BDH reduces diacetyl into (3R)-acetoin and (2R,3R)-2,3-BD, while racemic acetoin is reduced into (2R,3R)-2,3-BD and meso-2,3-BD
756608
Pseudomonas aeruginosa DSM 22644
?
-
-
-
-
additional information
no activity with (2S,3S)-2,3-butane-2,3-diol, but the (2R,3R)-2,3-butanediol dehydrogenase is also active with meso-2,3-butanediol. No activity with 2-butanol, 1,3-butanediol, 1,2-pentanediol, 1,3-propanediol, and glycerol in the oxidation reaction. And no activity with 2,4-pentanedione, butanone, 2,5-hexanedione, n-butanal and 1,3-dihydroxypropanone in the reduction reaction. Substrate specificity, overview. (2R,3R)-2,3-BDH reduces diacetyl into (3R)-acetoin and (2R,3R)-2,3-BD, while racemic acetoin is reduced into (2R,3R)-2,3-BD and meso-2,3-BD
756608
Pseudomonas aeruginosa CIP 104116
?
-
-
-
-
additional information
no activity with (2S,3S)-2,3-butane-2,3-diol, but the (2R,3R)-2,3-butanediol dehydrogenase is also active with meso-2,3-butanediol. No activity with 2-butanol, 1,3-butanediol, 1,2-pentanediol, 1,3-propanediol, and glycerol in the oxidation reaction. And no activity with 2,4-pentanedione, butanone, 2,5-hexanedione, n-butanal and 1,3-dihydroxypropanone in the reduction reaction. Substrate specificity, overview. (2R,3R)-2,3-BDH reduces diacetyl into (3R)-acetoin and (2R,3R)-2,3-BD, while racemic acetoin is reduced into (2R,3R)-2,3-BD and meso-2,3-BD
756608
Pseudomonas aeruginosa LMG 12228
?
-
-
-
-
additional information
no activity with (2S,3S)-2,3-butane-2,3-diol, but the (2R,3R)-2,3-butanediol dehydrogenase is also active with meso-2,3-butanediol. No activity with 2-butanol, 1,3-butanediol, 1,2-pentanediol, 1,3-propanediol, and glycerol in the oxidation reaction. And no activity with 2,4-pentanedione, butanone, 2,5-hexanedione, n-butanal and 1,3-dihydroxypropanone in the reduction reaction. Substrate specificity, overview. (2R,3R)-2,3-BDH reduces diacetyl into (3R)-acetoin and (2R,3R)-2,3-BD, while racemic acetoin is reduced into (2R,3R)-2,3-BD and meso-2,3-BD
756608
Pseudomonas aeruginosa JCM 14847
?
-
-
-
-
propane-1,2-diol + NAD+
-
756608
Pseudomonas aeruginosa
? + NADH + H+
-
-
-
?
rac acetoin + NADH + H+
-
756608
Pseudomonas aeruginosa
(2R,3R)-butane-2,3-diol + NAD+
-
-
-
?
rac acetoin + NADH + H+
-
756608
Pseudomonas aeruginosa
(2R,3S)-butane-2,3-diol + NAD+
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Pseudomonas aeruginosa
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
assay at
Pseudomonas aeruginosa
General Information
General Information
Commentary
Organism
malfunction
the amount of (2R,3R)-2,3-BD is highly reduced in a DELTAacoR mutant lacking the regulatory protein AcoR. The loss of locus pa4153, encoding (2R,3R)-2,3-BDH, has no effect on the ability of this strain to grow in (2S,3S)-2,3-BD but completely impairs its ability to utilize (2R,3R)-2,3-BD and meso-2,3-BD. The complementation of the pa4153 mutant strain with its gene successfully restores the growth ability. The DELTApa4153 PAO1 strain can grow in racemic acetoin, indicating that (2R,3R)-2,3-BDH contributes to 2,3-BD utilization by converting 2,3-BD into acetoin
Pseudomonas aeruginosa
physiological function
22,3-butanediol (2,3-BD) exists in three stereoisomeric forms: (2R,3R)-2,3-BD, meso-2,3-BD and (2S,3S)-2,3-BD. All three stereoisomers are transformed into acetoin by (2R,3R)-2,3-butanediol dehydrogenase (BDH) or (2S,3S)-2,3-BDH. Acetoin is cleaved to form acetyl-CoA and acetaldehyde by acetoin dehydrogenase enzyme system (AoDH ES). Genes encoding (2R,3R)-2,3-BDH, (2S,3S)-2,3-BDH and the E1 and E2 components of AoDH ES are identified as part of a 2,3-BD utilization operon. In addition, the regulatory protein AcoR promotes the expression of this operon using acetaldehyde, a cleavage product of acetoin, as its direct effector. Proposed model for 2,3-BD utilization in Pseudomonas aeruginosa strain PAO1 in downstream catabolic pathways, overview. Genes pa4148, pa4149, pa4150, pa4151, pa4152 and pa4153 comprise an operon responsible for 2,3-BD utilization, mutational analysis. Acetaldehyde is the direct inducer of the 2,3-BD utilization operon
Pseudomonas aeruginosa
General Information (protein specific)
General Information
Commentary
Organism
malfunction
the amount of (2R,3R)-2,3-BD is highly reduced in a DELTAacoR mutant lacking the regulatory protein AcoR. The loss of locus pa4153, encoding (2R,3R)-2,3-BDH, has no effect on the ability of this strain to grow in (2S,3S)-2,3-BD but completely impairs its ability to utilize (2R,3R)-2,3-BD and meso-2,3-BD. The complementation of the pa4153 mutant strain with its gene successfully restores the growth ability. The DELTApa4153 PAO1 strain can grow in racemic acetoin, indicating that (2R,3R)-2,3-BDH contributes to 2,3-BD utilization by converting 2,3-BD into acetoin
Pseudomonas aeruginosa
physiological function
22,3-butanediol (2,3-BD) exists in three stereoisomeric forms: (2R,3R)-2,3-BD, meso-2,3-BD and (2S,3S)-2,3-BD. All three stereoisomers are transformed into acetoin by (2R,3R)-2,3-butanediol dehydrogenase (BDH) or (2S,3S)-2,3-BDH. Acetoin is cleaved to form acetyl-CoA and acetaldehyde by acetoin dehydrogenase enzyme system (AoDH ES). Genes encoding (2R,3R)-2,3-BDH, (2S,3S)-2,3-BDH and the E1 and E2 components of AoDH ES are identified as part of a 2,3-BD utilization operon. In addition, the regulatory protein AcoR promotes the expression of this operon using acetaldehyde, a cleavage product of acetoin, as its direct effector. Proposed model for 2,3-BD utilization in Pseudomonas aeruginosa strain PAO1 in downstream catabolic pathways, overview. Genes pa4148, pa4149, pa4150, pa4151, pa4152 and pa4153 comprise an operon responsible for 2,3-BD utilization, mutational analysis. Acetaldehyde is the direct inducer of the 2,3-BD utilization operon
Pseudomonas aeruginosa
Other publictions for EC 1.1.1.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
760423
Kuang
New insights into two yeast B ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae YBA_08
Appl. Microbiol. Biotechnol.
104
6679-6692
2020
-
-
2
-
-
-
-
8
-
2
-
4
-
6
-
-
2
-
-
-
17
-
31
-
8
2
-
-
8
2
-
2
6
-
-
-
-
-
2
6
-
-
-
-
-
-
8
-
2
-
4
-
-
-
2
-
-
17
-
31
-
2
-
-
8
2
-
2
-
-
2
2
-
8
8
760761
Subramanian
Phylogenetics-based identific ...
no activity in Serratia marcescens
Biotechnol. Biofuels
13
186
2020
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
761810
Schilling
Engineering of the 2,3-butane ...
Paenibacillus polymyxa, Paenibacillus polymyxa DSM 365
Metab. Eng.
61
381-388
2020
-
-
-
-
1
-
-
-
-
-
-
2
-
4
-
-
-
-
-
-
-
-
4
-
2
1
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
762382
Muschallik
-
Synthesis of alpha-hydroxy ke ...
Alkalihalobacillus clausii, Alkalihalobacillus clausii DSM 8716T
RSC Adv.
10
12206-12216
2020
-
-
1
-
1
-
-
-
-
1
-
2
-
2
-
-
1
-
-
-
-
-
8
-
5
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
2
-
-
-
1
-
-
-
-
8
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
760463
Gong
-
Cloning, expression and chara ...
Bacillus thuringiensis serovar kurstaki, Bacillus thuringiensis serovar kurstaki ACCC 10066
Biocatal. Agricult. Biotechnol.
22
10137
2019
1
-
1
-
-
-
2
2
-
1
-
4
-
2
-
-
1
-
-
-
-
1
8
1
3
2
-
1
-
2
-
1
2
-
1
-
1
-
1
2
-
-
-
-
2
-
2
-
1
-
4
-
-
-
1
-
-
-
1
8
1
2
-
1
-
2
-
1
1
-
1
1
-
-
-
761834
Zhang
Synthetic engineering of Cory ...
Bacillus subtilis subsp. subtilis, Bacillus subtilis subsp. subtilis 168
Microb. Cell Fact.
18
128
2019
-
-
1
-
1
-
-
-
-
-
-
2
-
5
-
-
-
-
-
-
-
-
2
-
2
1
-
-
-
1
-
-
2
-
-
-
-
-
1
2
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
756608
Liu
2,3-Butanediol catabolism in ...
Pseudomonas aeruginosa, Pseudomonas aeruginosa ATCC 15692, Pseudomonas aeruginosa 1C, Pseudomonas aeruginosa PRS 101, Pseudomonas aeruginosa DSM 22644, Pseudomonas aeruginosa CIP 104116, Pseudomonas aeruginosa LMG 12228, Pseudomonas aeruginosa JCM 14847
Environ. Microbiol.
20
3927-3940
2018
-
-
1
-
-
-
-
1
-
1
-
16
-
11
-
-
-
-
-
-
8
-
48
-
5
1
-
-
-
1
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
1
-
1
-
16
-
-
-
-
-
-
8
-
48
-
1
-
-
-
1
-
-
-
-
2
2
-
-
-
760411
Dias
2,3-Butanediol production by ...
Paenibacillus brasilensis, Paenibacillus brasilensis PB24
Appl. Microbiol. Biotechnol.
102
8773-8782
2018
-
1
1
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
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4
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4
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1
1
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2
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4
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2
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1
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2
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762259
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Effect of deletion of 2,3-but ...
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2
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1
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2
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1
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2
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2
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737512
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Stereospecificity of Corynebac ...
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1
1
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4
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4
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1
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10
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4
4
737519
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Synthetic operon for (R,R)-2,3 ...
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2016
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1
1
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2
2
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2
2
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738345
Ge
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Contracted but effective: Prod ...
Bacillus licheniformis, Bacillus licheniformis MW3
Green Chem.
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20
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1
1
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1
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14
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20
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Bae
Efficient production of acetoi ...
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Sci. Rep.
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1
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737847
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Enhanced production of 2,3-but ...
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14
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2
1
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2
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1
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1
1
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4
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1
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2
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1
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14
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1
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2
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738800
Bai
Engineered Serratia marcescens ...
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4
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4
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12
2
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Identification and characteriz ...
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1
1
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4
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1
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1
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Characterization of a (2R,3R)- ...
Rhodococcus erythropolis, Rhodococcus erythropolis WZ010
Molecules
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19
2
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15
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3
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2
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2
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739571
Maddock
Substitutions at the cofactor ...
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21
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3
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21
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8
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1
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4
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A 2,3-butanediol dehydrogenase ...
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Crystallization and preliminar ...
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721345
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Carballo
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Crow
Properties of 2,3-butanediol d ...
Lactococcus lactis
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