Cloned (Comment) | Organism |
---|---|
mutant enzymes expressed in Escherichia coli | Columba livia |
Protein Variants | Comment | Organism |
---|---|---|
D235A | turnover number is 783.5fold lower than wild-type value | Columba livia |
D257A | turnover number is 28.5fold lower than wild-type value | Columba livia |
D258A | turnover number is 522fold lower than wild-type value | Columba livia |
E234A | turnover number is 1.3fold higher than wild-type value | Columba livia |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00179 | - |
NADP+ | pH 7.4, 25°C, mutant enzyme D235A | Columba livia | |
0.0018 | - |
NADP+ | pH 7.4, 25°C, mutant enzyme E234A | Columba livia | |
0.00207 | - |
NADP+ | pH 7.4, 25°C, Mn2+-activated, wild-type enzyme | Columba livia | |
0.00283 | - |
NADP+ | pH 7.4, 25°C, mutant enzyme D257A | Columba livia | |
0.00296 | - |
NADP+ | pH 7.4, 25°C, mutant enzyme D258A | Columba livia | |
0.05 | - |
(S)-malate | pH 7.4, 25°C, mutant enzyme D258A | Columba livia | |
0.08 | - |
(S)-malate | pH 7.4, 25°C, Mn2+-activated, wild-type enzyme | Columba livia | |
0.1 | - |
(S)-malate | pH 7.4, 25°C, mutant enzyme D235A | Columba livia | |
0.1 | - |
(S)-malate | pH 7.4, 25°C, mutant enzyme D257A | Columba livia | |
0.27 | - |
(S)-malate | pH 7.4, 25°C, Mg2+-activated, wild-type enzyme | Columba livia | |
13.33 | - |
(S)-malate | pH 7.4, 25°C, mutant enzyme E234A | Columba livia |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activates, KM: 0.16 mM for wild-type enzyme | Columba livia | |
Mn2+ | activates, KM: 0.00378 mM for wild-type enzyme | Columba livia |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Columba livia | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant mutant enzymes | Columba livia |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NADP+ | - |
Columba livia | pyruvate + CO2 + NADPH | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
(S)-malate | pH 7.4, 25°C, mutant enzyme D235A | Columba livia | |
0.005 | - |
(S)-malate | pH 7.4, 25°C, mutant enzyme E234A | Columba livia | |
0.006 | - |
(S)-malate | pH 7.4, 25°C, mutant enzyme D258A | Columba livia | |
0.3 | - |
(S)-malate | pH 7.4, 25°C, Mg2+-activated, wild-type enzyme | Columba livia | |
1.1 | - |
(S)-malate | pH 7.4, 25°C, mutant enzyme D257A | Columba livia | |
15 | - |
(S)-malate | pH 7.4, 25°C, Mn2+-activated, wild-type enzyme | Columba livia | |
31.34 | - |
(S)-malate | pH 7.4, 25°C, Mn2+-activated, wild-type enzyme | Columba livia | |
34.83 | - |
(S)-malate | pH 7.4, 25°C, Mg2+-activated, wild-type enzyme | Columba livia | |
46.44 | - |
(S)-malate | pH 7.4, 25°C, mutant enzyme E234A | Columba livia |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Columba livia |