Cloned (Comment) | Organism |
---|---|
expressed in Rhodotorula glutinis strain GM4 | Mucor circinelloides |
functional recombinant enzyme expression in Rhodotorula glutinis strain GM4, subcloning in Escherichia coli strain DH5alpha | Mucor circinelloides |
ME gene, DNA and amino acid sequence determination and analysis, recombinant enzyme expression of the enzyme in Rhodotorula glutinis strain GM4 using the PGK1 promoter, a strong constitutive promoter of yeast PGK1 gene, co-expression with the PGK1 gene from Saccharomyces cerevisiae | Mucor circinelloides |
Protein Variants | Comment | Organism |
---|---|---|
additional information | expression of the enzyme from Mucor circinelloides as a strategy to improve lipid content inside the Rhodotorula glutinis yeast cells, overview. Heterologous expression of NADP+-dependent ME involved in fatty acid biosynthesis increases the lipid accumulation in the oleaginous yeast Rhodotorula glutinis | Mucor circinelloides |
additional information | expression of malic enzyme from Mucor circinelloides as a strategy to improve lipid content inside the cells. The 26S rDNA and 5.8S rDNA gene fragments, with PGK1 gene from Saccharomyces cerevisiae strain 2.1445, isolated from Rhodotorula glutinis strain GM4 are used for homologous integration of the malc enzyme gene into Rhodotorula glutinis chromosome under the control of the constitutively highly expressed gene phosphoglycerate kinase 1 resulting in stable expression. Improvment of the lipid content by more than twofold. There are no significant differences in fatty acid profiles between the wild-type strain and the recombinant strain of Rhodotorula glutinis | Mucor circinelloides |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
sesamol | added to the medium, inhibits best at 9 mM; a specific inhibitor of the enzyme; strong inhibition at 10 mM | Mucor circinelloides |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NADP+ | Mucor circinelloides | - |
pyruvate + CO2 + NADPH | - |
? | |
(S)-malate + NADP+ | Mucor circinelloides | - |
pyruvate + CO2 + NADPH | - |
r | |
(S)-malate + NADP+ | Mucor circinelloides CBS 108.16 | - |
pyruvate + CO2 + NADPH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mucor circinelloides | - |
- |
- |
Mucor circinelloides | A6XP72 | - |
- |
Mucor circinelloides CBS 108.16 | A6XP72 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NADP+ | - |
Mucor circinelloides | pyruvate + CO2 + NADPH | - |
? | |
(S)-malate + NADP+ | - |
Mucor circinelloides | pyruvate + CO2 + NADPH | - |
r | |
(S)-malate + NADP+ | - |
Mucor circinelloides CBS 108.16 | pyruvate + CO2 + NADPH | - |
r |
Synonyms | Comment | Organism |
---|---|---|
malic enzyme | - |
Mucor circinelloides |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Mucor circinelloides |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Mucor circinelloides | |
NADP+ | dependent on | Mucor circinelloides | |
NADPH | - |
Mucor circinelloides |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in the fatty acid biosynthesis | Mucor circinelloides |