Cloned (Comment) | Organism |
---|---|
overexpression of wild-type enzyme and mutant enzyme, with modified cofactor specificity, in Saccharomyces cerevisiae strain CEN.PK 113-7D under control of the constitutive TDH3 promoter, coexpression with xylitol dehydrogenase from Galactocandida mastotermitis, The strain harboring the xylose reductase double mutant shows 42% enhanced ethanol yield compared to the reference strain harboring wild-type xylose reductase during anaerobic bioreactor conversions of xylose. Likewise, the yields of xylitol and glycerol are decreased by 52% and 57% respectively in the xylose reductase mutant strain | Yamadazyma tenuis |
Protein Variants | Comment | Organism |
---|---|---|
K274R/N276D | structure-guided site-directed mutagenesis, change of the coenzyme preference of the xyluose reductase about 170fold from NADPH in the wild-type to NADH, which, in spite of the structural modifications introduced, has retained the original catalytic efficiency for reduction of xylose by NADH | Yamadazyma tenuis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-xylose + NAD(P)H + H+ | Yamadazyma tenuis | - |
xylitol + NAD(P)+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Yamadazyma tenuis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-xylose + NAD(P)H + H+ | - |
Yamadazyma tenuis | xylitol + NAD(P)+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
xylose reductase | - |
Yamadazyma tenuis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD(P)H | - |
Yamadazyma tenuis |