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Literature summary for 1.1.1.62 extracted from

  • Wang, P.; Zheng, D.; Wang, Y.; Liang, R.
    One 3-oxoacyl-(acyl-carrier-protein) reductase functions as 17beta-hydroxysteroid dehydrogenase in the estrogen-degrading Pseudomonas putida SJTE-1 (2018), Biochem. Biophys. Res. Commun., 505, 910-916 .
    View publication on PubMed
Search for more literature for 1.1.1.62

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli BL21(DE3) strain Pseudomonas putida

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 5 mM, about 40% loss of activity Pseudomonas putida
Cu2+ 5 mM, about 70% loss of activity Pseudomonas putida
Zn2+ 5 mM, about 80% loss of activity Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.082
-
 17beta-estradiol pH 9.0, 37°C Pseudomonas putida
0.75
-
 estrone pH 9.0, 37°C Pseudomonas putida

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ weak stimulation Pseudomonas putida
Mn2+ weak stimulation Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
17beta-estradiol + NAD+ Pseudomonas putida
-
 estrone + NADH + H+
-
 ?
17beta-estradiol + NAD+ Pseudomonas putida SJTE-1
-
 estrone + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida ANI02794.1
-
-
Pseudomonas putida SJTE-1 ANI02794.1
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
17beta-estradiol + NAD+
-
 Pseudomonas putida estrone + NADH + H+
-
 ?
17beta-estradiol + NAD+ transformation efficiency of 17beta-estradiol into estrone is over 96.6% in five minutes Pseudomonas putida estrone + NADH + H+
-
 ?
17beta-estradiol + NAD+
-
 Pseudomonas putida SJTE-1 estrone + NADH + H+
-
 ?
17beta-estradiol + NAD+ transformation efficiency of 17beta-estradiol into estrone is over 96.6% in five minutes Pseudomonas putida SJTE-1 estrone + NADH + H+
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Pseudomonas putida

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 50 25°C: about 35% of maximal activity, 50°C: about 40% of maximal activity Pseudomonas putida

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.89
-
 estrone pH 9.0, 37°C Pseudomonas putida
2.1
-
 17beta-estradiol pH 9.0, 37°C Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Pseudomonas putida

pH Range

pH Minimum pH Maximum Comment Organism
6 11 pH 6.0: about 30% of maximal activity, pH 11.0: about 40% of maximal activity Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
NAD+ no activity with NADP+ Pseudomonas putida
NADH no activity with NADPH Pseudomonas putida

General Information

General Information Comment Organism
metabolism the enzyme plays an important role in estrogen metabolism Pseudomonas putida

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.19
-
 estrone pH 9.0, 37°C Pseudomonas putida
25.61
-
 17beta-estradiol pH 9.0, 37°C Pseudomonas putida