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Literature summary for 1.1.1.69 extracted from

  • Saichana, I.; Ano, Y.; Adachi, O.; Matsushita, K.; Toyama, H.
    Preparation of enzymes required for enzymatic quantification of 5-keto-D-gluconate and 2-keto-D-gluconate (2007), Biosci. Biotechnol. Biochem., 71, 2478-2486.
    View publication on PubMed

Application

Application Comment Organism
analysis enzymatic quantification of 5-keto-D-gluconate Gluconobacter oxydans

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3), expression levels vary from colony to colony, cells showing high enzyme expression tend to show less growth, expression system appears to be unstable as expression level decreases when subcultured for several times, best expression results with freshly transformed cells Gluconobacter oxydans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Gluconobacter oxydans production of 5-keto D-gluconate from D-gluconate ?
-
?

Organism

Organism UniProt Comment Textmining
Gluconobacter oxydans
-
NBRC3172, also Gluconobacter suboxydans IFO12528, genetically identical with Gluconobacter oxydans 621H
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Gluconobacter oxydans

Storage Stability

Storage Stability Organism
4Ā°C, 10 mM phosphate buffer, pH 6.0, no added 2-mercaptoethanol, fully stable for at least 3 days Gluconobacter oxydans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-keto D-gluconate + NADPH
-
Gluconobacter oxydans D-gluconate + NADP+
-
?
additional information production of 5-keto D-gluconate from D-gluconate Gluconobacter oxydans ?
-
?

Synonyms

Synonyms Comment Organism
5-keto D-gluconate reductase
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Gluconobacter oxydans
5KGA reductase
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Gluconobacter oxydans
5KGR
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Gluconobacter oxydans
GOX2187 gene product
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Gluconobacter oxydans

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Gluconobacter oxydans