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Literature summary for 1.1.1.72 extracted from

  • Richter, N.; Neumann, M.; Liese, A.; Wohlgemuth, R.; Eggert, T.; Hummel, W.
    Characterisation of a recombinant NADP-dependent glycerol dehydrogenase from Gluconobacter oxydans and its application in the production of L-glyceraldehyde (2009), ChemBioChem, 10, 1888-1896.
    View publication on PubMed
Search for more literature for 1.1.1.72

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed in Escherichia coli as a His-tagged fusion protein Gluconobacter oxydans

Inhibitors

Inhibitors Comment Organism Structure
NADPH inhibition if concentration is above 0.15 mM Gluconobacter oxydans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 NADP+ oxidation, pH 9.7, 37°C, Vmax: 2.1 micromol/min/mg Gluconobacter oxydans
0.04
-
 NADPH reduction, pH 7.0, 30°C, Vmax: 101 micromol/min/mg Gluconobacter oxydans
2.69
-
 D-glyceraldehyde reduction, pH 7.0, 30°C, Vmax: 77.9 micromol/min/mg Gluconobacter oxydans
193
-
 L-arabitol oxidation, pH 9.7, 37°C, Vmax: 8.67 micromol/min/mg Gluconobacter oxydans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37210
-
 calculated from cDNA Gluconobacter oxydans
39000
-
 SDS-PAGE Gluconobacter oxydans

Organism

Organism UniProt Comment Textmining
Gluconobacter oxydans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using Ni-NTA chromatography Gluconobacter oxydans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2,2-dimethyl-1,3-dioxolan-4-yl)methanol + NADP+ oxidation reaction. Specific activity: 0.03 micromol/min/mg Gluconobacter oxydans 2,2-dimethyl-1,3-dioxolane-4-carbaldehyde + NADPH
-
 ?
(2E)-pent-2-enal + NADPH + H+ reduction reaction: specific activity: 4.9 micromol/min/mg. Oxidation reaction specific activity: 0.74 micromol/min/mg Gluconobacter oxydans (2E)-pent-2-enol + NADP+
-
 ?
(2R)-2,4-dihydroxybutanal + NADPH + H+ reduction reaction. Specific activity: 46.6 micromol/min/mg Gluconobacter oxydans (2R)-2,4-dihydroxybutanol + NADP+
-
 ?
(2R,3S)-butane-1,2,3,4-tetrol + NADP+ oxidation reaction. Specific activity: 0.75 micromol/min/mg Gluconobacter oxydans ? + NADPH
-
 ?
(2R,3S,4R,5S)-hexane-1,2,3,4,5,6-hexol + NADP+ oxidation reaction. Specific activity: 1.08 micromol/min/mg Gluconobacter oxydans ? + NADPH
-
 ?
(2R,3S,4S)-pentane-1,2,3,4,5-pentol + NADP+ oxidation reaction. Specific activity: 0.25 micromol/min/mg Gluconobacter oxydans ? + NADPH
-
 ?
(2R,4S)-pentane-1,2,3,4,5-pentol + NADP+ oxidation reaction. Specific activity: 2.46 micromol/min/mg Gluconobacter oxydans ? + NADPH
-
 ?
(2S)-2,4-dihydroxybutanal + NADPH + H+ reduction reaction. Specific activity: 2.0 micromol/min/mg Gluconobacter oxydans (2S)-2,4-dihydroxybutanol + NADP+
-
 ?
(2S,3S)-butane-1,2,3,4-tetrol + NADP+ oxidation reaction. Specific activity: 0.9 micromol/min/mg Gluconobacter oxydans ? + NADPH
-
 ?
1,3-dihydroxypropan-2-one + NADPH + H+ reduction reaction. Specific activity: 0.5 micromol/min/mg Gluconobacter oxydans propane-1,2,3-triol + NADP+
-
 ?
1-phenylethane-1,2-diol + NADP+ oxidation reaction. Specific activity: 0.77 micromol/min/mg Gluconobacter oxydans ? + NADPH
-
 ?
1-phenylethanone + NADPH + H+ reduction reaction. Specific activity: 0.04 micromol/min/mg Gluconobacter oxydans 1-phenylethanol + NADP+
-
 ?
1-phenylpropanone + NADPH + H+ reduction reaction. Specific activity: 0.03 micromol/min/mg Gluconobacter oxydans 1-phenylpropanol + NADP+
-
 ?
2,4-difluorobenzaldehyde + NADPH + H+ reduction reaction. Specific activity: 19.6 micromol/min/mg Gluconobacter oxydans (2,4-difluorophenyl)methanol + NADP+
-
 ?
2,4-dihydroxybutanal + NADPH + H+ reduction reaction. Specific activity: 39.1 micromol/min/mg Gluconobacter oxydans 2,4-dihydroxybutanol + NADP+
-
 ?
2-methylbutanal + NADPH + H+ reduction reaction. Specific activity: 26.8 micromol/min/mg Gluconobacter oxydans 2-methylbutanol + NADP+
-
 ?
2-methylpentanal + NADPH + H+ reduction reaction. Specific activity: 25.7 micromol/min/mg Gluconobacter oxydans 2-methylpentanol + NADP+
-
 ?
2-methylpropanal + NADPH + H+ reduction reaction. Specific activity: 6.7 micromol/min/mg Gluconobacter oxydans 2-methylpropanol + NADP+
-
 ?
4-methoxybenzaldehyde + NADPH + H+ reduction reaction. Specific activity: 0.3 micromol/min/mg Gluconobacter oxydans (4-methoxyphenyl)methanol + NADP+
-
 ?
6-methylheptane-2,5-dione + NADPH + H+ reduction reaction. Specific activity: 0.03 micromol/min/mg Gluconobacter oxydans ? + NADP+
-
 ?
acetaldehyde + NADPH + H+ reduction reaction. Specific activity: 0.1 micromol/min/mg Gluconobacter oxydans ethanol + NADP+
-
 ?
benzaldehyde + NADPH + H+ reduction reaction. Specific activity: 13.8 micromol/min/mg Gluconobacter oxydans benzyl alcohol + NADP+
-
 ?
butanal + NADPH + H+ reduction reaction. Specific activity: 23.3 micromol/min/mg Gluconobacter oxydans butanol + NADP+
-
 ?
butane-1,2,4-triol + NADP+ oxidation reaction. Specific activity: 1.21 micromol/min/mg Gluconobacter oxydans ? + NADPH
-
 ?
butane-1,2-diol + NADP+ oxidation reaction. Specific activity: 0.66 micromol/min/mg Gluconobacter oxydans ? + NADPH
-
 ?
butane-1,3-diol + NADP+ oxidation reaction. Specific activity: 0.04 micromol/min/mg Gluconobacter oxydans ? + NADPH
-
 ?
butane-1,4-diol + NADP+ oxidation reaction. Specific activity: 0.21 micromol/min/mg Gluconobacter oxydans ? + NADPH
-
 ?
D-glyceraldehyde + NADPH + H+ enzyme favors reduction reaction Gluconobacter oxydans glycerol + NADP+
-
 ?
dodecanal + NADPH + H+ reduction reaction. Specific activity: 19.3 micromol/min/mg Gluconobacter oxydans dodecanol + NADP+
-
 ?
ethane-1,1-diol + NADP+ oxidation reaction. Specific activity: 0.5 micromol/min/mg Gluconobacter oxydans ? + NADPH
-
 ?
glycerol + NADP+ oxidation reaction. Specific activity: 0.17 micromol/min/mg Gluconobacter oxydans ? + NADPH + H+
-
 ?
hexan-2-ol + NADP+ oxidation reaction. Specific activity: 0.22 micromol/min/mg Gluconobacter oxydans hexan-2-one + NADPH
-
 ?
hexanal + NADPH + H+ reduction reaction. Specific activity: 31.9 micromol/min/mg Gluconobacter oxydans hexanol + NADP+
-
 r
hexane-1,2,3-triol + NADP+ oxidation reaction. Specific activity: 0.76 micromol/min/mg Gluconobacter oxydans ? + NADPH
-
 ?
hexane-1,2,6-triol + NADP+ oxidation reaction. Specific activity: 0.3 micromol/min/mg Gluconobacter oxydans ? + NADPH
-
 ?
hexane-1,2,6-triol + NADP+ oxidation reaction. Specific activity: 0.45 micromol/min/mg Gluconobacter oxydans ? + NADPH
-
 ?
hexane-1,6-diol + NADP+ oxidation reaction. Specific activity: 0.87 micromol/min/mg Gluconobacter oxydans ? + NADPH
-
 ?
L-arabitol + NADP+ oxidation reaction Gluconobacter oxydans ? + NADPH
-
 ?
methanediol + NADP+ oxidation reaction. Specific activity: 0.05 micromol/min/mg Gluconobacter oxydans ? + NADPH
-
 ?
nonanal + NADPH + H+ reduction reaction. Specific activity: 15.9 micromol/min/mg Gluconobacter oxydans nonanol + NADP+
-
 ?
octan-3-one + NADPH + H+ reduction reaction. Specific activity: 0.02 micromol/min/mg Gluconobacter oxydans octan-3-ol + NADP+
-
 ?
octanal + NADPH + H+ reduction reaction. Specific activity: 35.5 micromol/min/mg Gluconobacter oxydans octanol + NADP+
-
 ?
pentane-1,2-diol + NADP+ oxidation reaction. Specific activity: 0.39 micromol/min/mg Gluconobacter oxydans ? + NADPH
-
 ?
pentane-1,5-diol + NADP+ oxidation reaction. Specific activity: 0.59 micromol/min/mg Gluconobacter oxydans ? + NADPH
-
 ?
propanal + NADPH + H+ reduction reaction. Specific activity: 1.1 micromol/min/mg Gluconobacter oxydans propanol + NADP+
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 39000, SDS-PAGE Gluconobacter oxydans

Synonyms

Synonyms Comment Organism
GlyDH
-
 Gluconobacter oxydans
Gox1615
-
 Gluconobacter oxydans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 reduction assay at Gluconobacter oxydans
37
-
 oxidation assay at Gluconobacter oxydans
55
-
 optimal activity Gluconobacter oxydans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4 30 stable between Gluconobacter oxydans
50
-
 rapid inactivation occurs Gluconobacter oxydans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
 optimal activity for reduction Gluconobacter oxydans
7
-
 reduction assay at Gluconobacter oxydans
9.7
-
 oxidation assay at Gluconobacter oxydans
10
-
 optimal pH for axidation Gluconobacter oxydans

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 9.7 enzyme is stable and shows a residual activity of at least 82% Gluconobacter oxydans

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Gluconobacter oxydans
NADPH prefers NADPH over NADH. Using NADPH, a 200fold increase in activity compared to the activity achieved with the same concentration of NADH is observed Gluconobacter oxydans

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.4
-
 NADPH pH 7.0, 30 °C Gluconobacter oxydans