Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas putida |
expression in Escherichia coli | Acinetobacter calcoaceticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.017 | - |
3-methyl-2-buten-1-ol | - |
Acinetobacter calcoaceticus | |
0.046 | - |
benzyl alcohol | - |
Acinetobacter calcoaceticus | |
0.11 | - |
3-methyl-2-buten-1-ol | - |
Pseudomonas putida | |
0.13 | - |
benzyl alcohol | - |
Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzyl alcohol + NAD+ | Pseudomonas putida | - |
benzaldehyde + NADH | - |
? | |
benzyl alcohol + NAD+ | Acinetobacter calcoaceticus | - |
benzaldehyde + NADH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter calcoaceticus | - |
- |
- |
Pseudomonas putida | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-perillyl alcohol + NAD+ | 61% activity relative to benzyl alcohol | Acinetobacter calcoaceticus | (R)-perillaldehyde + NADH | - |
? | |
(R)-perillyl alcohol + NAD+ | 49% activity relative to benzyl alcohol | Pseudomonas putida | (R)-perillaldehyde + NADH | - |
? | |
(S)-perillyl alcohol + NAD+ | 85% activity relative to benzyl alcohol | Acinetobacter calcoaceticus | (S)-perillaldehyde + NADH | - |
? | |
(S)-perillyl alcohol + NAD+ | 68% activity relative to benzyl alcohol | Pseudomonas putida | (S)-perillaldehyde + NADH | - |
? | |
3-methyl-2-buten-1-ol + NAD+ | 210% activity relative to benzyl alcohol | Acinetobacter calcoaceticus | 3-methyl-2-buten-1-al + NADH | - |
? | |
3-methyl-2-buten-1-ol + NAD+ | 180% activity relative to benzyl alcohol | Pseudomonas putida | 3-methyl-2-buten-1-al + NADH | - |
r | |
4-aminobenzyl alcohol + NAD+ | 270% activity relative to benzyl alcohol | Acinetobacter calcoaceticus | 4-aminobenzaldehyde + NADH | - |
r | |
4-aminobenzyl alcohol + NAD+ | 280% activity relative to benzyl alcohol | Pseudomonas putida | 4-aminobenzaldehyde + NADH | - |
r | |
benzyl alcohol + NAD+ | - |
Pseudomonas putida | benzaldehyde + NADH | - |
? | |
benzyl alcohol + NAD+ | - |
Acinetobacter calcoaceticus | benzaldehyde + NADH | - |
? | |
geraniol + NAD+ | 68% activity relative to benzyl alcohol | Pseudomonas putida | geranial + NADH + H+ | - |
? | |
geraniol + NAD+ | 63% activity relative to benzyl alcohol | Acinetobacter calcoaceticus | geranial + NADH + H+ | - |
? | |
nerol + NAD+ | 25% activity relative to benzyl alcohol | Acinetobacter calcoaceticus | neral + NADH + H+ | - |
? | |
nerol + NAD+ | 77% activity relative to benzyl alcohol | Pseudomonas putida | neral + NADH + H+ | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
18 | - |
benzyl alcohol | - |
Pseudomonas putida | |
37 | - |
3-methyl-2-buten-1-ol | - |
Acinetobacter calcoaceticus | |
41 | - |
3-methyl-2-buten-1-ol | - |
Acinetobacter calcoaceticus | |
63 | - |
benzyl alcohol | - |
Acinetobacter calcoaceticus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pseudomonas putida | |
NAD+ | - |
Acinetobacter calcoaceticus | |
NADH | - |
Pseudomonas putida | |
NADH | - |
Acinetobacter calcoaceticus |