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Literature summary for 1.1.1.90 extracted from

  • Landete, J.M.; Rodriguez, H.; de Las Rivas, B.; Munoz, R.
    Characterization of a benzyl alcohol dehydrogenase from Lactobacillus plantarum WCFS1 (2008), J. Agric. Food Chem., 56, 4497-4503.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene lp_3054, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli strain JM109 Lactiplantibacillus plantarum WCFS1

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 10 mM, 76% inhibition Lactiplantibacillus plantarum
2-mercaptoethanol inhibits 76% at 10 mM Lactiplantibacillus plantarum WCFS1
Ag+ inhibits 58% at 10 mM Lactiplantibacillus plantarum WCFS1
AgNO3 10 mM, 58% inhibition Lactiplantibacillus plantarum
DMSO 10 mM, 20% inhibition Lactiplantibacillus plantarum
DMSO inhibits 20% at 10 mM Lactiplantibacillus plantarum WCFS1
DTT 10 mM, 84% inhibition Lactiplantibacillus plantarum
DTT inhibits 84% at 10 mM Lactiplantibacillus plantarum WCFS1
EDTA 10 mM, 22% inhibition Lactiplantibacillus plantarum
EDTA inhibits 22% at 10 mM Lactiplantibacillus plantarum WCFS1
SDS 10 mM, 86% inhibition Lactiplantibacillus plantarum
SDS inhibits 86% at 10 mM Lactiplantibacillus plantarum WCFS1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.23
-
benzyl alcohol
-
Lactiplantibacillus plantarum
0.23
-
benzyl alcohol pH 5.0, 30┬░C Lactiplantibacillus plantarum WCFS1

Metals/Ions

Metals/Ions Comment Organism Structure
CaCl2 10 mM, 1.3fold increase in activity Lactiplantibacillus plantarum
CaCl2 increases the enzyme activity by 27% at 10 mM Lactiplantibacillus plantarum WCFS1
CdCl2 10 mM, 1.3fold increase in activity Lactiplantibacillus plantarum
CdCl2 increases the enzyme activity by 25% at 10 mM Lactiplantibacillus plantarum WCFS1
KBr 10 mM, 1.1fold increase in activity Lactiplantibacillus plantarum
KBr increases the enzyme activity by 11% at 10 mM Lactiplantibacillus plantarum WCFS1
MgCl2 10 mM, 1.2fold increase in activity Lactiplantibacillus plantarum
MgCl2 increases the enzyme activity by 22% at 10 mM Lactiplantibacillus plantarum WCFS1
additional information no effects on the enzyme activity by KCl, NaCl, and NiCl2, and by 10 mM urea and Tween-80 Lactiplantibacillus plantarum WCFS1
Zn zinc-dependent alcohol dehydrogenase. All of the residues involved in zinc binding are conserved Lactiplantibacillus plantarum
Zn2+ dependent on, a catalytic zinc atom bound at the active site to the ligands Cys46, Asp49, His67, and Cys174 and a structural zinc atom bound to cysteine residues 97, 100, 103, and 111. All of the residues involved in zinc binding are conserved Lactiplantibacillus plantarum WCFS1

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
x * 40000, recombinant enzyme, SDS-PAGE Lactiplantibacillus plantarum WCFS1

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
benzyl alcohol + NAD(P)+ Lactiplantibacillus plantarum WCFS1
-
benzaldehyde + NAD(P)H + H+
-
?
benzyl alcohol + NAD(P)+ Lactiplantibacillus plantarum WCFS1 NCIMB 8826
-
benzaldehyde + NAD(P)H + H+
-
?
cinnamyl alcohol + NAD(P)+ Lactiplantibacillus plantarum WCFS1
-
cinnamyl aldehyde + NAD(P)H + H+
-
?
cinnamyl alcohol + NAD(P)+ Lactiplantibacillus plantarum WCFS1 NCIMB 8826
-
cinnamyl aldehyde + NAD(P)H + H+
-
?
coniferyl alcohol + NAD(P)+ Lactiplantibacillus plantarum WCFS1
-
coniferyl aldehyde + NAD(P)H + H+
-
?
coniferyl alcohol + NAD(P)+ Lactiplantibacillus plantarum WCFS1 NCIMB 8826
-
coniferyl aldehyde + NAD(P)H + H+
-
?
geraniol + NAD(P)+ Lactiplantibacillus plantarum WCFS1
-
geranial + NAD(P)H + H+
-
?
nerol + NAD(P)+ Lactiplantibacillus plantarum WCFS1
-
(2E)-3,7-dimethylocta-2,6-dienal + NAD(P)H + H+
-
?
phenethyl alcohol + NAD(P)+ Lactiplantibacillus plantarum WCFS1
-
phenylacetaldehyde + NAD(P)H + H+
-
?

Organism

Organism UniProt Comment Textmining
Lactiplantibacillus plantarum
-
-
-
Lactiplantibacillus plantarum WCFS1
-
gene lp_3054
-
Lactiplantibacillus plantarum WCFS1 NCIMB 8826
-
gene lp_3054
-

Purification (Commentary)

Purification (Comment) Organism
recombinant lp_3054 from Escherichia coli strain JM109 Lactiplantibacillus plantarum WCFS1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyl alcohol + NAD(P)+
-
Lactiplantibacillus plantarum WCFS1 benzaldehyde + NAD(P)H + H+
-
?
benzyl alcohol + NAD(P)+
-
Lactiplantibacillus plantarum WCFS1 NCIMB 8826 benzaldehyde + NAD(P)H + H+
-
?
benzyl alcohol + NAD+ less active in the presence of NADP+ than in the presence of NAD+ Lactiplantibacillus plantarum benzaldehyde + NADH
-
?
benzyl alcohol + NADP+ less active in the presence of NADP+ than in the presence of NAD+ Lactiplantibacillus plantarum benzaldehyde + NADPH
-
?
cinnamyl alcohol + NAD(P)+
-
Lactiplantibacillus plantarum WCFS1 cinnamyl aldehyde + NAD(P)H + H+
-
?
cinnamyl alcohol + NAD(P)+ 67% activity compared to activity with benzyl alcohol Lactiplantibacillus plantarum WCFS1 cinnamyl aldehyde + NAD(P)H + H+
-
?
cinnamyl alcohol + NAD(P)+
-
Lactiplantibacillus plantarum WCFS1 NCIMB 8826 cinnamyl aldehyde + NAD(P)H + H+
-
?
cinnamyl alcohol + NAD(P)+ 67% activity compared to activity with benzyl alcohol Lactiplantibacillus plantarum WCFS1 NCIMB 8826 cinnamyl aldehyde + NAD(P)H + H+
-
?
cinnamyl alcohol + NAD+ 67% of the activity with benzyl alcohol Lactiplantibacillus plantarum cinnamyl aldehyde + NADH + H+
-
?
coniferyl alcohol + NAD(P)+
-
Lactiplantibacillus plantarum WCFS1 coniferyl aldehyde + NAD(P)H + H+
-
?
coniferyl alcohol + NAD(P)+ 59% activity compared to activity with benzyl alcohol Lactiplantibacillus plantarum WCFS1 coniferyl aldehyde + NAD(P)H + H+
-
?
coniferyl alcohol + NAD(P)+
-
Lactiplantibacillus plantarum WCFS1 NCIMB 8826 coniferyl aldehyde + NAD(P)H + H+
-
?
coniferyl alcohol + NAD+ 59% of the activity with benzyl alcohol Lactiplantibacillus plantarum coniferyl aldehyde + NADH + H+
-
?
geraniol + NAD(P)+
-
Lactiplantibacillus plantarum WCFS1 geranial + NAD(P)H + H+
-
?
geraniol + NAD(P)+ 105% activity compared to activity with benzyl alcohol Lactiplantibacillus plantarum WCFS1 geranial + NAD(P)H + H+
-
?
geraniol + NAD+ 105% of the activity with benzyl alcohol Lactiplantibacillus plantarum (2Z)-3,7-dimethylocta-2,6-dienal + NADH + H+
-
?
additional information no activity with tyrosol, tryptophol, pyrogallol, catechol, phloroglucinol, ethanol, p-coumaric acid, and caffeic acid Lactiplantibacillus plantarum WCFS1 ?
-
?
additional information no activity with tyrosol, tryptophol, pyrogallol, catechol, phloroglucinol, ethanol, p-coumaric acid, and caffeic acid Lactiplantibacillus plantarum WCFS1 NCIMB 8826 ?
-
?
nerol + NAD(P)+
-
Lactiplantibacillus plantarum WCFS1 (2E)-3,7-dimethylocta-2,6-dienal + NAD(P)H + H+
-
?
nerol + NAD(P)+ 116% activity compared to activity with benzyl alcohol Lactiplantibacillus plantarum WCFS1 (2E)-3,7-dimethylocta-2,6-dienal + NAD(P)H + H+
-
?
nerol + NAD+ 116% of the activity with benzyl alcohol Lactiplantibacillus plantarum (2E)-3,7-dimethylocta-2,6-dienal + NADH + H+
-
?
phenethyl alcohol + NAD(P)+
-
Lactiplantibacillus plantarum WCFS1 phenylacetaldehyde + NAD(P)H + H+
-
?
phenethyl alcohol + NAD(P)+ 73% activity compared to activity with benzyl alcohol Lactiplantibacillus plantarum WCFS1 phenylacetaldehyde + NAD(P)H + H+
-
?
phenethyl alcohol + NAD+ 73% of the activity with benzyl alcohol Lactiplantibacillus plantarum phenylacetaldehyde + NADH + H+
-
?

Subunits

Subunits Comment Organism
? x * 40000, recombinant enzyme, SDS-PAGE Lactiplantibacillus plantarum WCFS1

Synonyms

Synonyms Comment Organism
BADH
-
Lactiplantibacillus plantarum WCFS1
benzyl alcohol dehydrogenase
-
Lactiplantibacillus plantarum WCFS1
More the enzyme is a member of the family of NAD(P)-dependent long-chain zinc-dependent alcohol dehydrogenases Lactiplantibacillus plantarum WCFS1

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
30
-
-
Lactiplantibacillus plantarum
30
-
-
Lactiplantibacillus plantarum WCFS1

Temperature Range [┬░C]

Temperature Minimum [┬░C] Temperature Maximum [┬░C] Comment Organism
20 50 20┬░C: about 60% of maximal activity, 50┬░C: about 70% of maximal activity Lactiplantibacillus plantarum
25 50 activity range Lactiplantibacillus plantarum WCFS1

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
30 50 the purified recombinant enzyme remains quite stable Lactiplantibacillus plantarum WCFS1
70
-
purified recombinant enzyme, BADH activity significantly decreases to 16% of maximal activity Lactiplantibacillus plantarum WCFS1

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4
-
benzyl alcohol pH 5.0, 30┬░C Lactiplantibacillus plantarum WCFS1

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Lactiplantibacillus plantarum
5
-
-
Lactiplantibacillus plantarum WCFS1

pH Range

pH Minimum pH Maximum Comment Organism
3.5 6 pH 3.5: about 60% of maximal activity, pH 6.0: about 45% of maximal activity Lactiplantibacillus plantarum

Cofactor

Cofactor Comment Organism Structure
NAD+ preferred cofactor compared to NADP+, all of the residues involved in cofactor binding are conserved. Residues Thr178 and Val203 interact with the nicotinamide ring, polypeptide backbone nitrogen atoms of residues Gly201 and Gly202 interact with an oxygen of the phosphate proximal to the nicotinamide group, and His47 binds the diphosphate of the NAD+ coenzyme. An Asp residue determines the specificity for NAD+ Lactiplantibacillus plantarum WCFS1
NADP+ preferred cofactor is NAD+, all of the residues involved in cofactor binding are conserved. An Asp residue determines the specificity for NAD+ Lactiplantibacillus plantarum WCFS1

pI Value

Organism Comment pI Value Maximum pI Value
Lactiplantibacillus plantarum WCFS1 sequence calculation
-
5.04