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Literature summary for 1.1.1.95 extracted from

  • Kito, K.; Tsutsumi, K.; Rai, V.; Theerawitaya, C.; Cha-Um, S.; Yamada-Kato, N.; Sakakibara, S.; Tanaka, Y.; Takabe, T.
    Isolation and functional characterization of 3-phosphoglycerate dehydrogenase involved in salt responses in sugar beet (2017), Protoplasma, 254, 2305-2313 .
    View publication on PubMed
Search for more literature for 1.1.1.95

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Beta vulgaris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.09355
-
 3-phosphooxypyruvate 30°C, pH 7.5 Beta vulgaris
0.1164
-
 3-phosphooxypyruvate 30°C, pH 7.5 Beta vulgaris
1.38
-
 3-phospho-D-glycerate 30°C, pH 9.0 Beta vulgaris
2.92
-
 3-phospho-D-glycerate 30°C, pH 9.0 Beta vulgaris

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-phospho-D-glycerate + NAD+ Beta vulgaris
-
 3-phosphooxypyruvate + NADH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Beta vulgaris A0A1Z4EAX3 L. cv. NK-219mm-0
-
Beta vulgaris A0A1Z4EAX4 L. cv. NK-219mm-0
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Beta vulgaris
-
petiole
-
 Beta vulgaris
-
root activity is high in storage root. After salt stress, enzyme (PGDH) activity is increased in leaf, petiole, and root Beta vulgaris
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-phospho-D-glycerate + NAD+
-
 Beta vulgaris 3-phosphooxypyruvate + NADH + H+
-
 r
3-phosphooxypyruvate + NADH + H+
-
 Beta vulgaris 3-phospho-D-glycerate + NAD+
-
 r

Synonyms

Synonyms Comment Organism
3-phosphoglycerate dehydrogenase
-
 Beta vulgaris
BvSHMTa
-
 Beta vulgaris
BvSHMTb
-
 Beta vulgaris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 substrates: 3-phosphooxypyruvate + NADH Beta vulgaris
9
-
 substrate: 3-phospho-D-glycerate + NAD+ Beta vulgaris
10
-
 substrate: 3-phospho-D-glycerate + NAD+ Beta vulgaris

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Beta vulgaris
NADH
-
 Beta vulgaris

Expression

Organism Comment Expression
Beta vulgaris mRNA transcriptional expression for BvPGDHb is significantly reduced under salt stress conditions down
Beta vulgaris mRNA transcriptional expression for BvPGDHa is significantly enhanced under salt stress conditions in both leaves and roots up

General Information

General Information Comment Organism
physiological function the enzyme plays an important role during salt stress in sugar beet Beta vulgaris