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Literature summary for 1.1.2.3 extracted from

  • Shkil, H.; Stoica, L.; Dmytruk, K.; Smutok, O.; Gonchar, M.; Sibirny, A.; Schuhmann, W.
    Bioelectrochemical detection of L-lactate respiration using genetically modified Hansenula polymorpha yeast cells overexpressing flavocytochrome b2 (2009), Bioelectrochemistry, 76, 175-179.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Hansenula polymorpha leading to enhanced L-lactate-dependent respiration compared to the wild-type cells Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
additional information for detection of the enzyme activity, recombinant enzyme is immobilized by means of a dialysis membrane onto various types of electrode materials in order to investigate the possibility of electrochemically detecting L-lactate respiratio, overview Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Saccharomyces cerevisiae 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-lactate + ferricytochrome c Saccharomyces cerevisiae
-
pyruvate + ferrocytochrome c + H+
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2
-
recombinant enzyme in cells of Hansenula polymorpha Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-lactate + ferricytochrome c
-
Saccharomyces cerevisiae pyruvate + ferrocytochrome c + H+
-
?

Synonyms

Synonyms Comment Organism
FC b2
-
Saccharomyces cerevisiae
flavocytochrome b2
-
Saccharomyces cerevisiae
L-lactate:cytochrome c-oxidoreductase
-
Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
assay at Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
cytochrome c
-
Saccharomyces cerevisiae