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Literature summary for 1.1.2.3 extracted from

  • Dmitruk, K.; Smutok, O.; Gonchar, M.; Sibirnyi, A.
    Construction of flavocytochrome b2-overproducing strains of the thermotolerant methylotrophic yeast Hansenula polymorpha (Pichia angusta) (2008), Microbiology, 77, 213-218.
    View publication on PubMed

Application

Application Comment Organism
synthesis the enzyme is potentially important for bioanalytical technologies for highly selective assays of L-lactate in biological fluids and foods Ogataea angusta

Cloned(Commentary)

Cloned (Comment) Organism
gene CYB2, expression in the recipient strain Hansenula polymorpha C-105 impaired in glucose repression and devoid of catalase activity, under the control of the strong Hansenula polymorpha alcohol oxidase promoter in a plasmid for multicopy integration, optimization of culture conditions for recombinant cells with increased FCb2 activity Ogataea angusta

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-lactate + ferricytochrome c Ogataea angusta
-
pyruvate + ferrocytochrome c + H+
-
?

Organism

Organism UniProt Comment Textmining
Ogataea angusta
-
i.e. Pichia angusa, gene CYB2
-

Source Tissue

Source Tissue Comment Organism Textmining
cell culture optimization of culture conditions for recombinant cells of strain C-105 expressing gene CYB2 and showing increased FCb2 activity Ogataea angusta
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3
-
cell-free extract from recombinant Hansenula polymorpha cell line expressing gene CYB2 Ogataea angusta

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-lactate + ferricytochrome c
-
Ogataea angusta pyruvate + ferrocytochrome c + H+
-
?

Synonyms

Synonyms Comment Organism
Fcb2
-
Ogataea angusta
flavocytochrome b
-
Ogataea angusta
L-lactate cytochrome c oxidoreductase
-
Ogataea angusta

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Ogataea angusta

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
thermostable enzyme Ogataea angusta

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Ogataea angusta

Cofactor

Cofactor Comment Organism Structure
cytochrome c
-
Ogataea angusta