BRENDA - Enzyme Database
show all sequences of 1.1.2.3

Chromate-reducing activity of Hansenula polymorpha recombinant cells over-producing flavocytochrome b2

Smutok, O.; Broda, D.; Smutok, H.; Dmytruk, K.; Gonchar, M.; Chemosphere 83, 449-454 (2011)

Data extracted from this reference:

Application
Application
Commentary
Organism
environmental protection
the reductive pathway of the enzyme resulting in formation of less toxic Cr(III)-species is suggested to be the most important among possible mechanisms for chromate biodetoxification
Ogataea angusta
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.09
-
(S)-lactate
pH 7.0, 20°C
Ogataea angusta
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(S)-lactate + 2 ferricytochrome c
Ogataea angusta
-
pyruvate + 2 ferrocytochrome c + 2 H+
-
-
?
(S)-lactate + 2 ferricytochrome c
Ogataea angusta C-105
-
pyruvate + 2 ferrocytochrome c + 2 H+
-
-
?
additional information
Ogataea angusta
chromate-reducing ability of enzyme-overproducing recombinant cells, the highest chromate-reducing activity of the cells is achieved in the presence of 2,6-dichlorophenolindophenol
?
-
-
-
additional information
Ogataea angusta C-105
chromate-reducing ability of enzyme-overproducing recombinant cells, the highest chromate-reducing activity of the cells is achieved in the presence of 2,6-dichlorophenolindophenol
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Ogataea angusta
-
-
-
Ogataea angusta C-105
-
-
-
Purification (Commentary)
Commentary
Organism
native enzyme to homogeneity
Ogataea angusta
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
9
-
purified native enzyme, pH 7.5, 20°C
Ogataea angusta
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-lactate + 2 ferricytochrome c
-
722066
Ogataea angusta
pyruvate + 2 ferrocytochrome c + 2 H+
-
-
-
?
(S)-lactate + 2 ferricytochrome c
-
722066
Ogataea angusta C-105
pyruvate + 2 ferrocytochrome c + 2 H+
-
-
-
?
(S)-lactate + K3[Fe(CN)6]
-
722066
Ogataea angusta
?
-
-
-
?
(S)-lactate + K3[Fe(CN)6]
-
722066
Ogataea angusta C-105
?
-
-
-
?
additional information
chromate-reducing ability of enzyme-overproducing recombinant cells, the highest chromate-reducing activity of the cells is achieved in the presence of 2,6-dichlorophenolindophenol
722066
Ogataea angusta
?
-
-
-
-
additional information
the enzyme has absolute specificity for L-lactate, yet is non-selective with respect to its electron acceptor
722066
Ogataea angusta
?
-
-
-
-
additional information
chromate-reducing ability of enzyme-overproducing recombinant cells, the highest chromate-reducing activity of the cells is achieved in the presence of 2,6-dichlorophenolindophenol
722066
Ogataea angusta C-105
?
-
-
-
-
additional information
the enzyme has absolute specificity for L-lactate, yet is non-selective with respect to its electron acceptor
722066
Ogataea angusta C-105
?
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
20
24
assay at
Ogataea angusta
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.3
7.5
assay at
Ogataea angusta
Cofactor
Cofactor
Commentary
Organism
Structure
2,6-dichlorophenolindophenol
-
Ogataea angusta
ferricytochrome c
-
Ogataea angusta
Application (protein specific)
Application
Commentary
Organism
environmental protection
the reductive pathway of the enzyme resulting in formation of less toxic Cr(III)-species is suggested to be the most important among possible mechanisms for chromate biodetoxification
Ogataea angusta
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
2,6-dichlorophenolindophenol
-
Ogataea angusta
ferricytochrome c
-
Ogataea angusta
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.09
-
(S)-lactate
pH 7.0, 20°C
Ogataea angusta
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(S)-lactate + 2 ferricytochrome c
Ogataea angusta
-
pyruvate + 2 ferrocytochrome c + 2 H+
-
-
?
(S)-lactate + 2 ferricytochrome c
Ogataea angusta C-105
-
pyruvate + 2 ferrocytochrome c + 2 H+
-
-
?
additional information
Ogataea angusta
chromate-reducing ability of enzyme-overproducing recombinant cells, the highest chromate-reducing activity of the cells is achieved in the presence of 2,6-dichlorophenolindophenol
?
-
-
-
additional information
Ogataea angusta C-105
chromate-reducing ability of enzyme-overproducing recombinant cells, the highest chromate-reducing activity of the cells is achieved in the presence of 2,6-dichlorophenolindophenol
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
native enzyme to homogeneity
Ogataea angusta
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
9
-
purified native enzyme, pH 7.5, 20°C
Ogataea angusta
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-lactate + 2 ferricytochrome c
-
722066
Ogataea angusta
pyruvate + 2 ferrocytochrome c + 2 H+
-
-
-
?
(S)-lactate + 2 ferricytochrome c
-
722066
Ogataea angusta C-105
pyruvate + 2 ferrocytochrome c + 2 H+
-
-
-
?
(S)-lactate + K3[Fe(CN)6]
-
722066
Ogataea angusta
?
-
-
-
?
(S)-lactate + K3[Fe(CN)6]
-
722066
Ogataea angusta C-105
?
-
-
-
?
additional information
chromate-reducing ability of enzyme-overproducing recombinant cells, the highest chromate-reducing activity of the cells is achieved in the presence of 2,6-dichlorophenolindophenol
722066
Ogataea angusta
?
-
-
-
-
additional information
the enzyme has absolute specificity for L-lactate, yet is non-selective with respect to its electron acceptor
722066
Ogataea angusta
?
-
-
-
-
additional information
chromate-reducing ability of enzyme-overproducing recombinant cells, the highest chromate-reducing activity of the cells is achieved in the presence of 2,6-dichlorophenolindophenol
722066
Ogataea angusta C-105
?
-
-
-
-
additional information
the enzyme has absolute specificity for L-lactate, yet is non-selective with respect to its electron acceptor
722066
Ogataea angusta C-105
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
20
24
assay at
Ogataea angusta
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.3
7.5
assay at
Ogataea angusta
Other publictions for EC 1.1.2.3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743420
Gillet
QM/MM study of l-lactate oxid ...
Saccharomyces cerevisiae
Phys. Chem. Chem. Phys.
18
15609-15618
2016
-
-
-
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3
-
-
1
1
-
-
1
-
1
-
-
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1
-
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-
-
2
1
1
-
-
3
1
-
-
5
-
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5
-
3
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1
1
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1
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2
1
1
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3
1
-
-
-
-
1
1
-
-
-
743494
Engqvist
Glycolate oxidase3, a glycola ...
Arabidopsis thaliana, Saccharomyces cerevisiae
Plant Physiol.
169
1042-1061
2015
-
-
1
-
2
-
-
1
2
-
-
2
-
4
-
-
-
-
-
2
-
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3
1
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1
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3
-
-
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1
3
-
2
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-
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-
1
2
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2
-
-
-
-
-
2
-
-
3
1
-
-
-
1
-
-
-
-
-
4
4
-
1
1
743862
Karkovska
L-Lactate-selective microbial ...
Ogataea angusta
Talanta
144
1195-1200
2015
-
1
1
-
1
-
-
-
-
-
-
-
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4
-
-
1
-
-
-
2
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1
1
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1
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1
1
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1
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1
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2
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1
1
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-
1
-
-
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-
-
-
-
-
743847
Smutok
Flavocytochrome b2-based enzy ...
Ogataea angusta, Ogataea angusta tr1
ScientificWorldJournal
2013
461284
2013
-
1
-
-
-
-
-
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1
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4
-
4
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1
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1
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6
1
1
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1
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4
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1
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4
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1
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4
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1
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1
-
6
1
1
-
-
-
1
-
-
-
-
1
1
-
-
-
722066
Smutok
Chromate-reducing activity of ...
Ogataea angusta, Ogataea angusta C-105
Chemosphere
83
449-454
2011
-
1
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-
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1
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4
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6
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1
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1
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8
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1
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1
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2
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1
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2
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1
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4
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1
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1
-
8
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1
-
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-
1
-
-
-
-
-
-
-
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-
722153
Lederer
Another look at the interactio ...
Saccharomyces cerevisiae
Eur. Biophys. J.
40
1283-1299
2011
-
-
-
1
-
-
-
13
2
-
-
1
-
5
-
-
-
1
-
-
-
-
1
1
1
-
-
5
1
-
-
4
-
-
-
-
-
-
4
1
-
-
-
-
-
13
2
-
-
1
-
-
-
-
-
-
-
-
1
1
1
-
-
5
1
-
-
-
-
-
-
-
-
-
695890
Mowat
Flavin-containing heme enzymes ...
Saccharomyces cerevisiae
Arch. Biochem. Biophys.
493
37-52
2010
-
-
-
-
4
-
-
-
-
-
1
1
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1
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1
1
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2
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2
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4
-
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1
1
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-
-
1
1
-
-
-
-
-
-
-
-
1
1
1
1
-
-
712523
Sakai
Functional replacement of yeas ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae D273-10B/A1
J. Biosci. Bioeng.
110
269-272
2010
-
-
1
-
-
-
-
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1
-
-
2
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3
-
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2
1
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2
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1
2
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1
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2
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2
1
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712759
Diep Le
Structural evidence for the fu ...
Saccharomyces cerevisiae
J. Mol. Biol.
400
518-530
2010
-
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-
1
-
-
-
-
2
-
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1
-
3
-
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1
1
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2
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2
1
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2
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1
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1
1
-
-
-
-
-
-
-
-
-
-
-
-
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721242
Arzi
-
Flavocytochrome b2 activity in ...
Satureja hortensis
Acta Hortic.
853
369-378
2010
-
-
-
-
-
-
1
2
-
-
-
1
-
1
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1
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2
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1
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2
2
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2
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1
2
2
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1
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1
-
-
2
-
-
-
-
-
1
-
-
-
-
1
1
-
-
-
696302
Le
Interdomain contacts in flavoc ...
Saccharomyces cerevisiae
Biochemistry
48
10803-10809
2009
-
-
-
-
14
-
-
15
1
1
-
1
-
2
-
-
-
-
-
-
-
-
4
1
1
-
-
26
1
-
-
3
-
-
-
-
-
-
3
-
14
-
-
-
-
15
1
1
-
1
-
-
-
-
-
-
-
-
4
1
1
-
-
26
1
-
-
-
-
1
1
-
-
-
696507
Shkil
Bioelectrochemical detection o ...
Saccharomyces cerevisiae
Bioelectrochemistry
76
175-179
2009
-
-
1
-
1
-
-
-
1
-
-
1
-
5
-
-
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1
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1
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1
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1
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1
1
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1
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1
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1
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1
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1
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-
1
-
-
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-
-
-
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697905
Tabacchi
L-lactate dehydrogenation in f ...
Saccharomyces cerevisiae
FEBS J.
276
2368-2380
2009
-
-
-
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-
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-
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1
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1
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3
1
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1
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1
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1
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3
1
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700066
Dmitruk
Construction of flavocytochrom ...
Ogataea angusta
Microbiology
77
213-218
2008
-
1
1
-
-
-
-
-
-
-
-
1
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4
-
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1
1
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1
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1
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1
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1
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1
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1
1
1
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1
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1
1
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1
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1
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1
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1
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-
672749
Hao
Pseudomonas stutzeri as a nove ...
Pseudomonas stutzeri
Biotechnol. Lett.
29
105-110
2007
-
-
-
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1
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1
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1
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1
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685107
Boubacar
Flavocytochrome b2: reactivity ...
Saccharomyces cerevisiae
Biochemistry
46
13080-13088
2007
-
-
1
-
1
-
-
-
1
-
-
-
-
3
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
1
-
-
-
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-
1
1
-
1
-
-
-
-
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1
-
-
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
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-
-
-
-
-
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-
685150
Cenas
Potentiometric and further kin ...
Saccharomyces cerevisiae
Biochemistry
46
4661-4670
2007
-
-
1
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1
-
6
18
-
-
-
-
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4
-
-
-
-
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-
-
1
1
-
-
-
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19
-
-
-
1
4
-
-
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-
1
1
-
1
-
-
6
4
18
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
19
-
-
-
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-
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-
-
-
-
685174
Tsai
Mechanistic and structural stu ...
Saccharomyces cerevisiae
Biochemistry
46
7844-7851
2007
-
-
1
1
1
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4
-
-
-
-
-
2
-
-
-
-
-
-
-
1
1
-
1
-
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4
1
-
-
-
-
-
-
-
-
1
-
1
1
-
-
-
-
4
-
-
-
-
-
-
-
-
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1
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685700
Smutok
Permeabilized cells of flavocy ...
Ogataea angusta
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675858
Smutok
-
Screening of yeasts producing ...
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Microbiology
75
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655069
Smutok
A novel L-lactate-selective bi ...
Ogataea angusta
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654138
Keyhani
-
Expression of L-lactate dehydr ...
Crocus sativus
Acta Hortic.
650
109-117
2004
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654766
Mowat
Altered substrate specificity ...
Saccharomyces cerevisiae
Biochemistry
43
9519-9526
2004
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654235
Gaida
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A new method of visualization ...
Ogataea angusta
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2003
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654520
Le
Epitope mapping for the monocl ...
Saccharomyces cerevisiae
Biochem. J.
373
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2003
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14
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654690
Sobrado
Solvent and primary deuterium ...
Saccharomyces cerevisiae
Biochemistry
42
15208-15214
2003
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348078
Cunane
Crystallographic study of the ...
Saccharomyces cerevisiae
Biochemistry
41
4264-4272
2002
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656765
Keyhani
Catalytic properties of three ...
Crocus sativus
Mol. Biol. Rep.
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2002
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3
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348077
Gondry
The catalytic role of tyrosine ...
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2001
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348076
Mowat
Kinetic and crystallographic s ...
Saccharomyces cerevisiae
Biochemistry
39
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11
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8
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6
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11
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8
1
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Sinclair
Re-design of Saccharomyces cer ...
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348073
Sharp
Deletions in the interdomain h ...
Saccharomyces cerevisiae
Biochemistry
35
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1996
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348074
Daff
New insights into the catalyti ...
Saccharomyces cerevisiae
Biochemistry
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3
1
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696188
Daff
Interaction of cytochrome c wi ...
Saccharomyces cerevisiae
Biochemistry
35
6351-6357
1996
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2
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348071
Gaume
Mutation to glutamine of histi ...
Saccharomyces cerevisiae
Biochimie
77
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1
3
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4
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2
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348072
Tegoni
X-ray structure of two complex ...
Saccharomyces cerevisiae
Biochemistry
34
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1
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3
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4
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3
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696075
Balme
Isolation and characterization ...
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Biochem. J.
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1
1
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3
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348069
Balme
On the rate of proton exchange ...
Saccharomyces cerevisiae
Protein Sci.
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1
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2
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4
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1
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1
4
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348070
Daff
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Strategic manipulation of the ...
Saccharomyces cerevisiae
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9
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5
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1
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3
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1
9
-
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348068
Silvestrini
Expression in Escherichia coli ...
Wickerhamomyces anomalus
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3
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348067
Brunt
Isolation and characterization ...
Saccharomyces cerevisiae
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3
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1
1
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1
1
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1
1
1
1
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1
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3
-
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-
-
-
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-
1
-
-
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348065
Lederer
-
On some aspects of the catalys ...
Saccharomyces cerevisiae
Flavins and Flavoproteins (Proc. Int. Symp. , 10th, Meeting Date 1990, Curti, B. , Ronchi S. , Zanetti, G. , eds. ) de Gruyter, Berlin, New York
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4
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4
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3
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1
3
1
2
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4
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1
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4
-
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4
-
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348064
Genet
The carbanion of nitroethane i ...
Saccharomyces cerevisiae
Biochem. J.
266
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3
1
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3
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1
2
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2
1
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1
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1
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-
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3
-
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-
-
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348066
Tegoni
Inhibition of L-lactate: cytoc ...
Saccharomyces cerevisiae, Wickerhamomyces anomalus
Eur. J. Biochem.
190
329-342
1990
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2
4
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7
-
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2
1
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2
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2
1
4
-
2
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2
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7
-
-
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699506
Xia
Molecular structure of flavocy ...
Saccharomyces cerevisiae
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1990
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2
1
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1
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348063
Black
Structural basis for the kinet ...
Saccharomyces cerevisiae, Wickerhamomyces anomalus
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2
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5
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2
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2
2
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2
1
2
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5
-
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348062
Ghrir
Primary structure of flavocyto ...
Saccharomyces cerevisiae
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-
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3
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1
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1
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1
-
1
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1
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3
-
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-
-
-
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348060
Blazy
A standard high-yield purifica ...
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-
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-
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1
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1
1
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1
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1
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1
1
2
1
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1
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1
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1
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1
1
1
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1
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1
1
2
1
-
-
-
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-
-
-
-
-
-
-
-
-
348061
Labeyrie
Flavocytochrome b 2 or L-lacta ...
Saccharomyces cerevisiae, Wickerhamomyces anomalus
Methods Enzymol.
53
238-256
1978
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6
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287801
Hatefi
-
Metal-containing flavoprotein ...
Saccharomyces cerevisiae, Wickerhamomyces anomalus
The Enzymes, 3rd Ed. (Boyer, P. D. , ed. )
13
175-297
1976
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2
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348058
Jacq
Cytochrome b2 from bakers yeas ...
Saccharomyces cerevisiae
Eur. J. Biochem.
41
311-320
1974
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1
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348057
Guiard
Yeast L-lactate dehydrogenase ...
Saccharomyces cerevisiae
Eur. J. Biochem.
34
241-247
1973
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1
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348056
Pritchard
An NAD+-independent L-lactate ...
Rhizopus oryzae
Biochim. Biophys. Acta
250
25-34
1971
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