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Literature summary for 1.1.2.3 extracted from

  • Smutok, O.; Broda, D.; Smutok, H.; Dmytruk, K.; Gonchar, M.
    Chromate-reducing activity of Hansenula polymorpha recombinant cells over-producing flavocytochrome b2 (2011), Chemosphere, 83, 449-454.
    View publication on PubMed

Application

Application Comment Organism
environmental protection the reductive pathway of the enzyme resulting in formation of less toxic Cr(III)-species is suggested to be the most important among possible mechanisms for chromate biodetoxification Ogataea angusta

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.09
-
(S)-lactate pH 7.0, 20°C Ogataea angusta

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-lactate + 2 ferricytochrome c Ogataea angusta
-
pyruvate + 2 ferrocytochrome c + 2 H+
-
?
(S)-lactate + 2 ferricytochrome c Ogataea angusta C-105
-
pyruvate + 2 ferrocytochrome c + 2 H+
-
?
additional information Ogataea angusta chromate-reducing ability of enzyme-overproducing recombinant cells, the highest chromate-reducing activity of the cells is achieved in the presence of 2,6-dichlorophenolindophenol ?
-
?
additional information Ogataea angusta C-105 chromate-reducing ability of enzyme-overproducing recombinant cells, the highest chromate-reducing activity of the cells is achieved in the presence of 2,6-dichlorophenolindophenol ?
-
?

Organism

Organism UniProt Comment Textmining
Ogataea angusta
-
-
-
Ogataea angusta C-105
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme to homogeneity Ogataea angusta

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
9
-
purified native enzyme, pH 7.5, 20°C Ogataea angusta

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-lactate + 2 ferricytochrome c
-
Ogataea angusta pyruvate + 2 ferrocytochrome c + 2 H+
-
?
(S)-lactate + 2 ferricytochrome c
-
Ogataea angusta C-105 pyruvate + 2 ferrocytochrome c + 2 H+
-
?
(S)-lactate + K3[Fe(CN)6]
-
Ogataea angusta ?
-
?
(S)-lactate + K3[Fe(CN)6]
-
Ogataea angusta C-105 ?
-
?
additional information chromate-reducing ability of enzyme-overproducing recombinant cells, the highest chromate-reducing activity of the cells is achieved in the presence of 2,6-dichlorophenolindophenol Ogataea angusta ?
-
?
additional information the enzyme has absolute specificity for L-lactate, yet is non-selective with respect to its electron acceptor Ogataea angusta ?
-
?
additional information chromate-reducing ability of enzyme-overproducing recombinant cells, the highest chromate-reducing activity of the cells is achieved in the presence of 2,6-dichlorophenolindophenol Ogataea angusta C-105 ?
-
?
additional information the enzyme has absolute specificity for L-lactate, yet is non-selective with respect to its electron acceptor Ogataea angusta C-105 ?
-
?

Synonyms

Synonyms Comment Organism
FC b2
-
Ogataea angusta
flavocytochrome b2
-
Ogataea angusta
L-lactate:cytochrome c-oxidoreductase
-
Ogataea angusta

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20 24 assay at Ogataea angusta

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3 7.5 assay at Ogataea angusta

Cofactor

Cofactor Comment Organism Structure
2,6-dichlorophenolindophenol
-
Ogataea angusta
ferricytochrome c
-
Ogataea angusta