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Literature summary for 1.1.2.4 extracted from

  • Pohanka, M.; Zboril, P.; Pikula, J.
    D-Lactate dehydrogenase (cytochrome) from saccharomyces cerevisiae purification by fast protein liquid chromatography (2009), Pol. J. Chem., 83, 415-420.
No PubMed abstract available

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Saccharomyces cerevisiae 5739
-

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by fast protein liquid chromatography on hydroxylapatite and on AH-Sepharose with covalently bound 4-hydroxy-a cyanocinnamic acid Saccharomyces cerevisiae
hydroxylapatite column chromatography and 4-hydroxy-alpha-cyanocinnamic acid-AH-Sepharose column chromatography Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [Āµmol/min/mg] Specific Activity Maximum [Āµmol/min/mg] Comment Organism
0.203
-
after purification by 4-hydroxy-alpha-cyanocinnamic acid-AH-Sepharose column chromatography, pH and temperature not specified in the publication Saccharomyces cerevisiae
0.342
-
after purification by hydroxylapatite column chromatography and 4-hydroxy-alpha-cyanocinnamic acid-AH-Sepharose column chromatography, pH and temperature not specified in the publication Saccharomyces cerevisiae
3.38
-
enzyme purified on H-Sepharose with covalently bound 4-hydroxy-alpha cyanocinnamic acid Saccharomyces cerevisiae
5.71
-
combination of chromatography on hydroxylapatite and H-Sepharose with covalently bound 4-hydroxy-alpha cyanocinnamic acid Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-lactate + 2 ferricytochrome
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Saccharomyces cerevisiae pyruvate + 2 ferrocytochrome
-
?
D-lactate + ferricytochrome c
-
Saccharomyces cerevisiae pyruvate + ferrocytochrome c
-
?
additional information no cross-reactivity with L-lactic acid Saccharomyces cerevisiae ?
-
?