Cloned (Comment) | Organism |
---|---|
heterologously expressed in Escherichia coli BL21 (DE3) | Trametes ochracea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trametes ochracea | - |
- |
- |
Trametes ochracea MB 49 | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
complete oxidation of D-glucose using pyranose oxidase as the synthesizing biocatalyst and laccase for redox mediator regeneration is described quantitatively to increase both stability and productivity | Trametes ochracea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + 1,4-benzoquinone | - |
Trametes ochracea | 2-dehydro-D-glucose + 1,4-hydroquinone | - |
? | |
D-glucose + 1,4-benzoquinone | - |
Trametes ochracea MB 49 | 2-dehydro-D-glucose + 1,4-hydroquinone | - |
? | |
D-glucose + 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) cation radical | - |
Trametes ochracea | 2-dehydro-D-glucose + 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | - |
? | |
D-glucose + 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) cation radical | - |
Trametes ochracea MB 49 | 2-dehydro-D-glucose + 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | - |
? | |
D-glucose + O2 | - |
Trametes ochracea | 2-dehydro-D-glucose + H2O2 | - |
? | |
D-glucose + O2 | - |
Trametes ochracea MB 49 | 2-dehydro-D-glucose + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
P2O | - |
Trametes ochracea |
pyranose 2-Oxidase | - |
Trametes ochracea |
pyranose oxidase | - |
Trametes ochracea |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.7 | - |
D-glucose | substrate 1,4-benzoquinone (100 mM D-glucose), pH 4.5 | Trametes ochracea | |
5.4 | - |
D-glucose | substrate D-glucose (air saturated), pH 6.5 | Trametes ochracea | |
7.1 | - |
D-glucose | substrate O2 (100 mM D-glucose), pH 6.5 | Trametes ochracea |