Inhibitors | Comment | Organism | Structure |
---|---|---|---|
HgCl2 | 30.8% inhibition at 1 mM | Stenotrophomonas maltophilia | |
KCN | 65.2% inhibition at 1 mM, complete inhibition at 10 mM | Stenotrophomonas maltophilia | |
N-bromosuccinimide | complete inhibition at 1 mM | Stenotrophomonas maltophilia | |
NaN3 | 18.5% inhibition at 1 mM | Stenotrophomonas maltophilia | |
Pb(CH3COO)2 | 18.7% inhibition at 1 mM | Stenotrophomonas maltophilia |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.044 | - |
Inosine | - |
Stenotrophomonas maltophilia |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | 2 g non-heme iron per mol of enzyme, contains also a heme component | Stenotrophomonas maltophilia |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
14000 | - |
alpha,beta,gamma,delta 1 * 76000 + 1 * 33000 + 1 * 18000 + 1 * 14000, SDS-PAGE, subunits beta and delta appear to be linked together by disulfide bonds | Stenotrophomonas maltophilia |
18000 | - |
alpha,beta,gamma,delta 1 * 76000 + 1 * 33000 + 1 * 18000 + 1 * 14000, SDS-PAGE, subunits beta and delta appear to be linked together by disulfide bonds | Stenotrophomonas maltophilia |
33000 | - |
alpha,beta,gamma,delta 1 * 76000 + 1 * 33000 + 1 * 18000 + 1 * 14000, SDS-PAGE, subunits beta and delta appear to be linked together by disulfide bonds | Stenotrophomonas maltophilia |
76000 | - |
alpha,beta,gamma,delta 1 * 76000 + 1 * 33000 + 1 * 18000 + 1 * 14000, SDS-PAGE, subunits beta and delta appear to be linked together by disulfide bonds | Stenotrophomonas maltophilia |
130000 | - |
gel filtration | Stenotrophomonas maltophilia |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
inosine + O2 | Stenotrophomonas maltophilia | oxidation of various nucleosides, but no oxidation of nucleotides, bases and ribose | 9-riburonosylhypoxanthine + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Stenotrophomonas maltophilia | - |
- |
- |
Stenotrophomonas maltophilia LB-86 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, chromatography techniques | Stenotrophomonas maltophilia |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Stenotrophomonas maltophilia | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2'-deoxyadenosine + O2 | 81.4% of the activity compared to inosine | Stenotrophomonas maltophilia | 9-(2-deoxy-beta-D-erythro-pentofuranuronosyl)-9H-purin-6-amine + H2O | - |
? | |
adenosine + O2 | 97.3% of the activity compared to inosine | Stenotrophomonas maltophilia | 9-riburonosyladenine + H2O | - |
? | |
adenosine + O2 | 97.3% of the activity compared to inosine | Stenotrophomonas maltophilia LB-86 | 9-riburonosyladenine + H2O | - |
? | |
cytidine + O2 | 77.3% of the activity compared to inosine | Stenotrophomonas maltophilia | 1-riburonosylcytosine + H2O | - |
? | |
cytidine + O2 | 77.3% of the activity compared to inosine | Stenotrophomonas maltophilia LB-86 | 1-riburonosylcytosine + H2O | - |
? | |
deoxycytidine + O2 | 77.3% of the activity compared to inosine | Stenotrophomonas maltophilia | 1-riburonosylcytosine + H2O | - |
? | |
deoxyguanosine + O2 | 92.3% of the activity compared to inosine | Stenotrophomonas maltophilia | 9-riburonosyldeoxyguanine + H2O | - |
? | |
deoxyinosine + O2 | 80.7% of the activity compared to inosine | Stenotrophomonas maltophilia | 9-riburonosyldeoxyhypoxanthine + H2O | - |
? | |
guanosine + O2 | 121% of the activity compared to inosine | Stenotrophomonas maltophilia | 9-riburonosylguanine + H2O | - |
? | |
guanosine + O2 | 121% of the activity compared to inosine | Stenotrophomonas maltophilia LB-86 | 9-riburonosylguanine + H2O | - |
? | |
inosine + O2 | oxidation of various nucleosides, but no oxidation of nucleotides, bases and ribose | Stenotrophomonas maltophilia | 9-riburonosylhypoxanthine + H2O | - |
? | |
thymidine + O2 | 55.8% of the activity compared to inosine | Stenotrophomonas maltophilia | 1-riburonosylthymine + H2O | - |
? | |
uridine + O2 | - |
Stenotrophomonas maltophilia | 1-riburonosyluracil + H2O | - |
? | |
uridine + O2 | - |
Stenotrophomonas maltophilia LB-86 | 1-riburonosyluracil + H2O | - |
? | |
xanthosine + O2 | best substrate tested, 125% of the activity compared to inosine | Stenotrophomonas maltophilia | 9-riburonosylxanthine + H2O | - |
? | |
xanthosine + O2 | best substrate tested, 125% of the activity compared to inosine | Stenotrophomonas maltophilia LB-86 | 9-riburonosylxanthine + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotetramer | alpha,beta,gamma,delta 1 * 76000 + 1 * 33000 + 1 * 18000 + 1 * 14000, SDS-PAGE, subunits beta and delta appear to be linked together by disulfide bonds | Stenotrophomonas maltophilia |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
stable below, complete loss of activity at 75°C | Stenotrophomonas maltophilia |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 6 | - |
Stenotrophomonas maltophilia |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 6 | stable for 1 h at 37°C | Stenotrophomonas maltophilia |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | 1 FAD covalently bound to the alpha subunit | Stenotrophomonas maltophilia |