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Literature summary for 1.1.3.38 extracted from

  • Ewing, T.A.; van Noord, A.; Paul, C.E.; van Berkel, W.J.H.
    A xylenol orange-based screening assay for the substrate specificity of flavin-dependent para-phenol oxidases (2018), Molecules, 23, 164 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.3.38

Application

Application Comment Organism
analysis development of a screening assay for the substrate specificity of para-phenol oxidases based on the detection of hydrogen peroxide using the ferric-xylenol orange complex method Penicillium simplicissimum

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Penicillium simplicissimum

Protein Variants

Protein Variants Comment Organism
C470L mutant displays similar activity to the wild-type enzyme with the substrates vanillyl alcohol, chavicol aund eugenol, but no activity with linear 4-alkylphenols Penicillium simplicissimum
F424G mutant does not contain any flavin after purification Penicillium simplicissimum
I468V mutant displays similar activity to the wild-type enzyme with the substrates vanillyl alcohol, chavicol aund eugenol, but no activity with linear 4-alkylphenols Penicillium simplicissimum
L316M mutant displays substrate specificity profile similar to wild-type Penicillium simplicissimum
T457Q mutant shows about 3fold increased activity towards vanillyl alcohol, but decrease in activity with all other substrates Penicillium simplicissimum
T459I mutant displays substrate specificity profile similar to wild-type Penicillium simplicissimum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.129
-
 Vanillyl alcohol His-tagged recombinant enzyme, pH 7.5, 25°C Penicillium simplicissimum

Organism

Organism UniProt Comment Textmining
Penicillium simplicissimum P56216
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-cyclohexylphenol + O2
-
 Penicillium simplicissimum ?
-
 ?
4-cyclopentylphenol + O2
-
 Penicillium simplicissimum 4-(1-cyclopenten-1-yl)phenol
-
 ?
4-ethylphenol + O2 + H2O
-
 Penicillium simplicissimum 1-(4'-hydroxyphenyl)ethanol + H2O2
-
 ?
4-n-propylphenol + O2
-
 Penicillium simplicissimum ?
-
 ?
chavicol + O2 + H2O
-
 Penicillium simplicissimum coumaryl alcohol + H2O2
-
 ?
eugenol + O2 + H2O
-
 Penicillium simplicissimum coniferyl alcohol + H2O2
-
 ?
vanillyl alcohol + O2
-
 Penicillium simplicissimum vanillin + H2O2
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5
-
 Vanillyl alcohol His-tagged recombinant enzyme, pH 7.5, 25°C Penicillium simplicissimum
3.7
-
 4-cyclopentylphenol His-tagged recombinant enzyme, pH 7.5, 25°C Penicillium simplicissimum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
19
-
 Vanillyl alcohol His-tagged recombinant enzyme, pH 7.5, 25°C Penicillium simplicissimum