Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris KM71H | Aspergillus niger |
Crystallization (Comment) | Organism |
---|---|
vapor diffusion sitting drop method | Aspergillus niger |
Protein Variants | Comment | Organism |
---|---|---|
T30V/I94V/A162T | 2.9fold increase in kcat/Km, decrease in t1/2(60°C) by 1.5°C | Aspergillus niger |
T30V/I94V/A162T/R537K/M556V | 4.0fol2.6fold increase in kcat/Km, increase in t1/2(60°C) by 5.25°C | Aspergillus niger |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
14.98 | - |
beta-D-glucose | pH 5.5, temperature not specified in the publication, mutant enzyme T30V/I94V/A162T | Aspergillus niger | |
18.54 | - |
beta-D-glucose | pH 5.5, temperature not specified in the publication, mutant enzyme T30V/I94V/A162T/R537K/M556V | Aspergillus niger | |
19.76 | - |
beta-D-glucose | pH 5.5, temperature not specified in the publication, mutant enzyme T30V/R37K/I94V/V106I/A162T/M556V | Aspergillus niger | |
28.26 | - |
beta-D-glucose | pH 5.5, temperature not specified in the publication, wild-type enzyme | Aspergillus niger |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-D-glucose + O2 | Aspergillus niger | - |
D-glucono-1,5-lactone + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | P13006 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-D-glucose + O2 | - |
Aspergillus niger | D-glucono-1,5-lactone + H2O2 | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
t1/2: 10.5 min (wild-type enzyme), 9.0 (mutant enzyme T30V/I94V/A162T), 11.74 (mutant enzyme T30V/I94V/A162T/R537K/M556V), 15.75 (mutant enzyme T30V/R37K/I94V/V106I/A162T/M556V) | Aspergillus niger |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
189.38 | - |
beta-D-glucose | pH 5.5, temperature not specified in the publication, wild-type enzyme | Aspergillus niger | |
291.82 | - |
beta-D-glucose | pH 5.5, temperature not specified in the publication, mutant enzyme T30V/I94V/A162T | Aspergillus niger | |
345.16 | - |
beta-D-glucose | pH 5.5, temperature not specified in the publication, mutant enzyme T30V/R37K/I94V/V106I/A162T/M556V | Aspergillus niger | |
498.34 | - |
beta-D-glucose | pH 5.5, temperature not specified in the publication, mutant enzyme T30V/I94V/A162T/R537K/M556V | Aspergillus niger |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.7 | - |
beta-D-glucose | pH 5.5, temperature not specified in the publication, wild-type enzyme | Aspergillus niger | |
17.5 | - |
beta-D-glucose | pH 5.5, temperature not specified in the publication, mutant enzyme T30V/R37K/I94V/V106I/A162T/M556V | Aspergillus niger | |
19.5 | - |
beta-D-glucose | pH 5.5, temperature not specified in the publication, mutant enzyme T30V/I94V/A162T | Aspergillus niger | |
26.9 | - |
beta-D-glucose | pH 5.5, temperature not specified in the publication, mutant enzyme T30V/I94V/A162T/R537K/M556V | Aspergillus niger |