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Literature summary for 1.1.3.6 extracted from
- Refolding of a novel cholesterol oxidase from Pimelobacter simplex reveals dehydrogenation activity (2017), Protein Expr. Purif., 139, 1-7 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli | Pimelobacter simplex |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pimelobacter simplex | A0A0A1DJ35 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| effective method for the preparation of active PsChO3 using urea as denaturing agent, combined with his-tag trapping and on-column refolding. Using this method, the enzyme is successfully refolded from inclusion bodies expressed in Escherichia coli | Pimelobacter simplex |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cholesterol + O2 | - |
Pimelobacter simplex | cholest-5-en-3-one + H2O2 | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ChO3 | - |
Pimelobacter simplex |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Pimelobacter simplex |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 45 | 25°C: about 85% of maximal activity, 45°C: about 40% of maximal activity | Pimelobacter simplex |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Pimelobacter simplex |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 9 | pH 6.5: about 50% of maximal activity, pH 9.0: about 50% of maximal activity | Pimelobacter simplex |
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Release 2023.1 (January 2023)
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