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Literature summary for 1.1.3.7 extracted from

  • Tamaru, Y.; Umezawa, K.; Yoshida, M.
    Characterization of an aryl-alcohol oxidase from the plant saprophytic basidiomycete Coprinopsis cinerea with broad substrate specificity against aromatic alcohols (2018), Biotechnol. Lett., 40, 1077-1086 .
    View publication on PubMed
Search for more literature for 1.1.3.7

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Coprinopsis cinerea

Protein Variants

Protein Variants Comment Organism
L416F mutation decreases the activity with aromatic alcohols but maintains the activity with trans,trans-2,4-hexadien-1-ol Coprinopsis cinerea
L416W mutation decreases the activity with aromatic alcohols but maintains the activity with trans,trans-2,4-hexadien-1-ol Coprinopsis cinerea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0012
-
 benzyl alcohol wild-type, pH 7, 25°C Coprinopsis cinerea
0.004
-
 3-anisyl alcohol wild-type, pH 7, 25°C Coprinopsis cinerea
0.0057
-
 3-Hydroxybenzyl alcohol wild-type, pH 7, 25°C Coprinopsis cinerea
0.0059
-
 benzyl alcohol mutant L416W, pH 7, 25°C Coprinopsis cinerea
0.0063
-
 Vanillyl alcohol wild-type, pH 7, 25°C Coprinopsis cinerea
0.007
-
 (2E,4E)-hexa-2,4-dien-1-ol mutant L416W, pH 7, 25°C Coprinopsis cinerea
0.0076
-
 4-anisyl alcohol mutant L416W, pH 7, 25°C Coprinopsis cinerea
0.0079
-
 benzyl alcohol mutant L416F, pH 7, 25°C Coprinopsis cinerea
0.012
-
 4-anisyl alcohol wild-type, pH 7, 25°C Coprinopsis cinerea
0.0156
-
 (2E,4E)-hexa-2,4-dien-1-ol wild-type, pH 7, 25°C Coprinopsis cinerea
0.0164
-
 (2E,4E)-hexa-2,4-dien-1-ol mutant L416F, pH 7, 25°C Coprinopsis cinerea
0.019
-
 4-anisyl alcohol mutant L416F, pH 7, 25°C Coprinopsis cinerea
0.031
-
 3-anisyl alcohol mutant L416W, pH 7, 25°C Coprinopsis cinerea
0.034
-
 3-anisyl alcohol mutant L416F, pH 7, 25°C Coprinopsis cinerea
0.042
-
 isovanillyl alcohol wild-type, pH 7, 25°C Coprinopsis cinerea
0.0483
-
 veratryl alcohol wild-type, pH 7, 25°C Coprinopsis cinerea
0.0563
-
 4-Hydroxybenzyl alcohol wild-type, pH 7, 25°C Coprinopsis cinerea
0.154
-
 isovanillyl alcohol mutant L416W, pH 7, 25°C Coprinopsis cinerea
0.163
-
 4-Hydroxybenzyl alcohol mutant L416F, pH 7, 25°C Coprinopsis cinerea
0.279
-
 3-Hydroxybenzyl alcohol mutant L416W, pH 7, 25°C Coprinopsis cinerea
0.301
-
 3-Hydroxybenzyl alcohol mutant L416F, pH 7, 25°C Coprinopsis cinerea
0.318
-
 isovanillyl alcohol mutant L416F, pH 7, 25°C Coprinopsis cinerea
0.419
-
 Vanillyl alcohol mutant L416W, pH 7, 25°C Coprinopsis cinerea
0.424
-
 veratryl alcohol mutant L416F, pH 7, 25°C Coprinopsis cinerea
0.466
-
 4-Hydroxybenzyl alcohol mutant L416W, pH 7, 25°C Coprinopsis cinerea
0.686
-
 veratryl alcohol mutant L416W, pH 7, 25°C Coprinopsis cinerea
0.98
-
 Vanillyl alcohol mutant L416F, pH 7, 25°C Coprinopsis cinerea

Organism

Organism UniProt Comment Textmining
Coprinopsis cinerea A0A2Z5WP81
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein the amino acid sequence contains four and seven possible sites of N-glycosylation and O-glycosylation, respectively Coprinopsis cinerea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,4E)-hexa-2,4-dien-1-ol + O2 282% of the activity with benzyl alcohol Coprinopsis cinerea ? + H2O2
-
 ?
2,4-dimethoxybenzyl alcohol + O2 286% of the activity with benzyl alcohol Coprinopsis cinerea 2,4-dimethoxybenzyl aldehyde + H2O2
-
 ?
2-anisyl alcohol + O2 96% of the activity with benzyl alcohol Coprinopsis cinerea 2-anisyl aldehyde + H2O2
-
 ?
3-anisyl alcohol + O2 297% of the activity with benzyl alcohol Coprinopsis cinerea 3-anisyl aldehyde + H2O2
-
 ?
3-hydroxybenzyl alcohol + O2 221% of the activity with benzyl alcohol Coprinopsis cinerea 3-hydroxybenzyl aldehyde + H2O2
-
 ?
4-anisyl alcohol + O2 298% of the activity with benzyl alcohol Coprinopsis cinerea 4-anisyl aldehyde + H2O2
-
 ?
4-hydroxybenzyl alcohol + O2 207% of the activity with benzyl alcohol Coprinopsis cinerea 4-hydroxybenzyl aldehyde + H2O2
-
 ?
benzyl alcohol + O2
-
 Coprinopsis cinerea benzaldehyde + H2O2
-
 ?
benzyl alcohol + O2
-
 Coprinopsis cinerea benzyl aldehyde + H2O2
-
 ?
cinnamyl alcohol + O2 289% of the activity with benzyl alcohol Coprinopsis cinerea cinnamyl aldehyde + H2O2
-
 ?
isovanillyl alcohol + O2 73% of the activity with benzyl alcohol Coprinopsis cinerea isovanillyl aldehyde + H2O2
-
 ?
additional information no activity with mono- and disaccharides. No electron acceptor: DCIP Coprinopsis cinerea ?
-
-
vanillyl alcohol + O2
-
 Coprinopsis cinerea vanillyl aldehyde + H2O2
-
 ?
veratryl alcohol + O2 318% of the activity with benzyl alcohol Coprinopsis cinerea veratryl aldehyde + H2O2
-
 ?

Subunits

Subunits Comment Organism
? x * 62600, calculated from sequence, x * 65600, SDS-PAGE of endoH-treated protein Coprinopsis cinerea

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.54
-
 4-Hydroxybenzyl alcohol mutant L416W, pH 7, 25°C Coprinopsis cinerea
0.81
-
 Vanillyl alcohol mutant L416W, pH 7, 25°C Coprinopsis cinerea
1.17
-
 3-Hydroxybenzyl alcohol mutant L416W, pH 7, 25°C Coprinopsis cinerea
2.05
-
 isovanillyl alcohol mutant L416W, pH 7, 25°C Coprinopsis cinerea
2.96
-
 veratryl alcohol mutant L416W, pH 7, 25°C Coprinopsis cinerea
3.49
-
 4-anisyl alcohol mutant L416W, pH 7, 25°C Coprinopsis cinerea
3.84
-
 benzyl alcohol mutant L416W, pH 7, 25°C Coprinopsis cinerea
4.81
-
 4-Hydroxybenzyl alcohol wild-type, pH 7, 25°C Coprinopsis cinerea
6.13
-
 benzyl alcohol wild-type, pH 7, 25°C Coprinopsis cinerea
7.02
-
 isovanillyl alcohol wild-type, pH 7, 25°C Coprinopsis cinerea
7.5
-
 3-Hydroxybenzyl alcohol mutant L416F, pH 7, 25°C Coprinopsis cinerea
7.66
-
 3-anisyl alcohol wild-type, pH 7, 25°C Coprinopsis cinerea
8.03
-
 benzyl alcohol mutant L416F, pH 7, 25°C Coprinopsis cinerea
8.67
-
 isovanillyl alcohol mutant L416F, pH 7, 25°C Coprinopsis cinerea
8.77
-
 4-Hydroxybenzyl alcohol mutant L416F, pH 7, 25°C Coprinopsis cinerea
9.91
-
 3-Hydroxybenzyl alcohol wild-type, pH 7, 25°C Coprinopsis cinerea
11.3
-
 3-anisyl alcohol mutant L416W, pH 7, 25°C Coprinopsis cinerea
12.4
-
 veratryl alcohol mutant L416F, pH 7, 25°C Coprinopsis cinerea
12.4
-
 3-anisyl alcohol mutant L416F, pH 7, 25°C Coprinopsis cinerea
12.5
-
 4-anisyl alcohol wild-type, pH 7, 25°C Coprinopsis cinerea
13.2
-
 veratryl alcohol wild-type, pH 7, 25°C Coprinopsis cinerea
14.2
-
 4-anisyl alcohol mutant L416F, pH 7, 25°C Coprinopsis cinerea
14.7
-
 Vanillyl alcohol wild-type, pH 7, 25°C Coprinopsis cinerea
17.3
-
 (2E,4E)-hexa-2,4-dien-1-ol mutant L416W, pH 7, 25°C Coprinopsis cinerea
21.3
-
 Vanillyl alcohol mutant L416F, pH 7, 25°C Coprinopsis cinerea
48.3
-
 (2E,4E)-hexa-2,4-dien-1-ol wild-type, pH 7, 25°C Coprinopsis cinerea
68.4
-
 (2E,4E)-hexa-2,4-dien-1-ol mutant L416F, pH 7, 25°C Coprinopsis cinerea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Coprinopsis cinerea

pH Range

pH Minimum pH Maximum Comment Organism
6 8
-
 Coprinopsis cinerea

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Coprinopsis cinerea

pI Value

Organism Comment pI Value Maximum pI Value
Coprinopsis cinerea calculated from sequence
-
 6.7

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.2
-
 4-Hydroxybenzyl alcohol mutant L416W, pH 7, 25°C Coprinopsis cinerea
1.9
-
 Vanillyl alcohol mutant L416W, pH 7, 25°C Coprinopsis cinerea
4.2
-
 3-Hydroxybenzyl alcohol mutant L416W, pH 7, 25°C Coprinopsis cinerea
4.3
-
 veratryl alcohol mutant L416W, pH 7, 25°C Coprinopsis cinerea
13.3
-
 isovanillyl alcohol mutant L416W, pH 7, 25°C Coprinopsis cinerea
21.8
-
 Vanillyl alcohol mutant L416F, pH 7, 25°C Coprinopsis cinerea
25
-
 3-Hydroxybenzyl alcohol mutant L416F, pH 7, 25°C Coprinopsis cinerea
27.3
-
 isovanillyl alcohol mutant L416F, pH 7, 25°C Coprinopsis cinerea
29.3
-
 veratryl alcohol mutant L416F, pH 7, 25°C Coprinopsis cinerea
53.9
-
 4-Hydroxybenzyl alcohol mutant L416F, pH 7, 25°C Coprinopsis cinerea
85.5
-
 4-Hydroxybenzyl alcohol wild-type, pH 7, 25°C Coprinopsis cinerea
167
-
 isovanillyl alcohol wild-type, pH 7, 25°C Coprinopsis cinerea
273
-
 veratryl alcohol wild-type, pH 7, 25°C Coprinopsis cinerea
361
-
 3-anisyl alcohol mutant L416F, pH 7, 25°C Coprinopsis cinerea
367
-
 3-anisyl alcohol mutant L416W, pH 7, 25°C Coprinopsis cinerea
461
-
 4-anisyl alcohol mutant L416W, pH 7, 25°C Coprinopsis cinerea
665
-
 benzyl alcohol mutant L416W, pH 7, 25°C Coprinopsis cinerea
740
-
 4-anisyl alcohol mutant L416F, pH 7, 25°C Coprinopsis cinerea
1020
-
 benzyl alcohol mutant L416F, pH 7, 25°C Coprinopsis cinerea
1080
-
 4-anisyl alcohol wild-type, pH 7, 25°C Coprinopsis cinerea
1750
-
 3-Hydroxybenzyl alcohol wild-type, pH 7, 25°C Coprinopsis cinerea
1940
-
 3-anisyl alcohol wild-type, pH 7, 25°C Coprinopsis cinerea
2350
-
 Vanillyl alcohol wild-type, pH 7, 25°C Coprinopsis cinerea
2460
-
 (2E,4E)-hexa-2,4-dien-1-ol mutant L416W, pH 7, 25°C Coprinopsis cinerea
3100
-
 (2E,4E)-hexa-2,4-dien-1-ol wild-type, pH 7, 25°C Coprinopsis cinerea
4180
-
 (2E,4E)-hexa-2,4-dien-1-ol mutant L416F, pH 7, 25°C Coprinopsis cinerea
5060
-
 benzyl alcohol wild-type, pH 7, 25°C Coprinopsis cinerea