Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu(NO3)2 | 0.19 mM concentration 12% inhibition, 0.38 mM concentration 76% inhibition and 0.89 mM concentration 100% inhibition | Gluconobacter oxydans | |
CuCl2 * 2H2O | 0.19 mM concentration 16% inhibition, 0.38 mM concentration 92% inhibition and 0.89 mM concentration 100% inhibition | Gluconobacter oxydans | |
CuSO4 | 0.19 mM concentration 20% inhibition, 0.38 mM concentration 80% inhibition and 0.89 mM concentration 100% inhibition | Gluconobacter oxydans | |
Fe2(SO4)3 | sub mM concentration slight inhibition | Gluconobacter oxydans | |
MgCl2 * 6H2O | sub mM concentration slight inhibition | Gluconobacter oxydans | |
MgSO4 * 7H2O | sub mM concentration slight inhibition | Gluconobacter oxydans | |
MnCl2 * 4H2O | 0.38 mM and 0.89 mM concentration 100% inhibition | Gluconobacter oxydans | |
MnSO4 * 4-6H2O | 0.38 mM and 0.89 mM concentration 100% inhibition | Gluconobacter oxydans | |
additional information | not inhibitory are: EDTA, sodium azide, monoiodoacetate, p-chloromercuribenzoate, NaHAsO4, sodium fluoride, KCN, hydroxyl ammonium sulfate, hydroxylamine, hydrazine monohydrate, hydrazinium dichloride, and 1,4-benzoquinone | Gluconobacter oxydans | |
NiSO4 | sub mM concentration slight inhibition | Gluconobacter oxydans | |
TiCl2 | sub mM concentration slight inhibition | Gluconobacter oxydans | |
ZnCl2 | sub mM concentration slight inhibition | Gluconobacter oxydans | |
ZnSO4 | sub mM concentration slight inhibition | Gluconobacter oxydans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Gluconobacter oxydans | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
16500 | - |
alpha,beta,gamma, 1 * 61000 + 1 * 32500 + 1 * 16500, the largest is a flavoprotein, gel filtration with 0.1% SDS in buffer and SDS-PAGE | Gluconobacter oxydans |
32500 | - |
alpha,beta,gamma, 1 * 61000 + 1 * 32500 + 1 * 16500, the largest is a flavoprotein, gel filtration with 0.1% SDS in buffer and SDS-PAGE | Gluconobacter oxydans |
61000 | - |
alpha,beta,gamma, 1 * 61000 + 1 * 32500 + 1 * 16500, the largest is a flavoprotein, gel filtration with 0.1% SDS in buffer and SDS-PAGE | Gluconobacter oxydans |
110000 | - |
gel filtration | Gluconobacter oxydans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-gulono-1,4-lactone + O2 | Gluconobacter oxydans | - |
L-xylo-hex-3-ulonolactone + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gluconobacter oxydans | - |
strain DSM 4025 | - |
Purification (Comment) | Organism |
---|---|
- |
Gluconobacter oxydans |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-gulono-1,4-lactone + O2 = L-xylo-hex-2-ulono-1,4-lactone + H2O2 | product isomerizes spontaneously to L-ascorbate | Gluconobacter oxydans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + phenazine methosulfate + O2 | 23.4% as active as L-gulono-1,4-lactone | Gluconobacter oxydans | D-glucosone + ? | - |
? | |
D-mannose + phenazine methosulfate + O2 | 6.4% as active as L-gulono-1,4-lactone | Gluconobacter oxydans | ? | - |
? | |
D-xylose + phenazine methosulfate + O2 | 6.4% as active as L-gulono-1,4-lactone | Gluconobacter oxydans | D-xylosone + ? | - |
? | |
L-glucurono-1,4-lactone + phenazine methosulfate + O2 | 6.4% as active as L-gulono-1,4-lactone | Gluconobacter oxydans | ? | - |
? | |
L-gulono-1,4-lactone + 2,6-dichlorophenolindophenol + O2 | enzyme is a dehydrogenase, not an oxidase | Gluconobacter oxydans | L-ascorbic acid + ? | - |
? | |
L-gulono-1,4-lactone + O2 | - |
Gluconobacter oxydans | L-xylo-hex-3-ulonolactone + H2O2 | - |
? | |
L-gulono-1,4-lactone + O2 | - |
Gluconobacter oxydans | L-xylo-hex-3-ulonolactone + H2O2 | product isomerizes spontaneously to L-ascorbate | ? | |
L-gulono-1,4-lactone + phenazine methosulfate + O2 | enzyme is a dehydrogenase, not an oxidase | Gluconobacter oxydans | L-ascorbic acid + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | alpha,beta,gamma, 1 * 61000 + 1 * 32500 + 1 * 16500, the largest is a flavoprotein, gel filtration with 0.1% SDS in buffer and SDS-PAGE | Gluconobacter oxydans |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 55 | no detectable optimum | Gluconobacter oxydans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
55 | 53% activity at 55 C | Gluconobacter oxydans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Gluconobacter oxydans |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9.2 | - |
Gluconobacter oxydans |