BRENDA - Enzyme Database show
show all sequences of 1.1.5.12

Membrane-bound D-lactate dehydrogenase from Escherichia coli: Purification and properties

Pratt, E.; Fung, L.; Flower, J.; Ho, C.; Biochemistry 18, 312-316 (1979)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
Triton X-100
5fold increase in activtiy of purified detergent-free enzyme
Escherichia coli
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.4
-
(R)-lactate
pH 8.0, 23°C; presence of Triton X-100, pH 8.0, 23°C
Escherichia coli
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Escherichia coli
16020
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
75000
-
x * 75000, SDS-PAGE
Escherichia coli
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
-
-
Escherichia coli W3110trpA33
-
-
-
Purification (Commentary)
Commentary
Organism
from sonicated cell extracts, purifiaction in the presence of 1% Triton X-100 and 0.1% sodium dodecyl sulfate
Escherichia coli
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
230
-
pH 8.0, 23°C
Escherichia coli
Storage Stability
Storage Stability
Organism
-20°C, detergent-free enzyme is destroyed by freezing and thawing
Escherichia coli
4°C, stable up to 6 months
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(R)-lactate + oxidized phenazine methosulfate
-
740029
Escherichia coli
pyruvate + reduced phenazine methosulfate
-
-
-
?
(R)-lactate + oxidized phenazine methosulfate
-
740029
Escherichia coli W3110trpA33
pyruvate + reduced phenazine methosulfate
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 75000, SDS-PAGE
Escherichia coli
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
60
-
1 h, 35% loss of activity, more readily inactivated in presence of Triton X-100
Escherichia coli
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
9.5
-
Escherichia coli
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
visible absorption spectrum of the purified enzyme shows a broad peak at around 450 nm and a shoulder at around 480 nm
Escherichia coli
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
Triton X-100
5fold increase in activtiy of purified detergent-free enzyme
Escherichia coli
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
visible absorption spectrum of the purified enzyme shows a broad peak at around 450 nm and a shoulder at around 480 nm
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.4
-
(R)-lactate
pH 8.0, 23°C; presence of Triton X-100, pH 8.0, 23°C
Escherichia coli
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Escherichia coli
16020
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
75000
-
x * 75000, SDS-PAGE
Escherichia coli
Purification (Commentary) (protein specific)
Commentary
Organism
from sonicated cell extracts, purifiaction in the presence of 1% Triton X-100 and 0.1% sodium dodecyl sulfate
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
230
-
pH 8.0, 23°C
Escherichia coli
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, detergent-free enzyme is destroyed by freezing and thawing
Escherichia coli
4°C, stable up to 6 months
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(R)-lactate + oxidized phenazine methosulfate
-
740029
Escherichia coli
pyruvate + reduced phenazine methosulfate
-
-
-
?
(R)-lactate + oxidized phenazine methosulfate
-
740029
Escherichia coli W3110trpA33
pyruvate + reduced phenazine methosulfate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 75000, SDS-PAGE
Escherichia coli
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
60
-
1 h, 35% loss of activity, more readily inactivated in presence of Triton X-100
Escherichia coli
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
9.5
-
Escherichia coli
Other publictions for EC 1.1.5.12
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
727253
Kato
Quinone-dependent D-lactate de ...
Corynebacterium glutamicum, Corynebacterium glutamicum ATCC 13032
BMC Microbiol.
10
321
2010
-
-
-
-
-
-
-
1
-
-
-
-
-
25
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
740031
Peersen
Site-specific incorporation of ...
Escherichia coli
Biochemistry
29
3256-3262
1990
-
-
-
1
22
-
-
8
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
22
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
740030
Matsushita
D-Lactate oxidation and genera ...
Escherichia coli, Escherichia coli GR19N
Biochemistry
25
2321-2327
1986
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
1
-
-
-
-
-
4
-
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-
-
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-
-
-
-
-
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-
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-
-
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
740029
Pratt
Membrane-bound D-lactate dehyd ...
Escherichia coli, Escherichia coli W3110trpA33
Biochemistry
18
312-316
1979
1
-
-
-
-
-
-
1
1
-
1
-
-
2
-
-
1
-
-
-
1
2
2
1
-
-
1
-
1
-
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
1
-
1
-
-
-
-
1
-
-
1
2
2
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
740028
Futai
Membrane D-lactate dehydrogena ...
Escherichia coli
Biochemistry
12
2468-2474
1973
1
-
-
-
-
-
1
4
1
-
2
-
-
1
-
-
1
-
-
-
-
2
3
1
-
-
-
-
1
-
-
1
-
-
-
1
-
-
1
-
-
-
-
1
-
4
1
-
2
-
-
-
-
1
-
-
-
2
3
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
740643
Kohn
Mechanisms of active transport ...
Escherichia coli, Escherichia coli ML 308-225
J. Biol. Chem.
248
7012-7017
1973
-
-
-
-
-
-
2
4
-
-
1
-
-
3
-
-
1
-
-
-
-
-
8
1
-
-
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
-
-
2
-
4
-
-
1
-
-
-
-
1
-
-
-
-
8
1
-
-
-
-
2
-
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