Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Gluconobacter japonicus | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
16000 | - |
1 * 60000 + 1 * 49000 + 1 * 16000, calculated from amino acid sequence | Gluconobacter japonicus |
18000 | - |
1 * 68000 + 1 * 51000 + 1 * 18000, SDS-PAGE | Gluconobacter japonicus |
49000 | - |
1 * 60000 + 1 * 49000 + 1 * 16000, calculated from amino acid sequence | Gluconobacter japonicus |
51000 | - |
1 * 68000 + 1 * 51000 + 1 * 18000, SDS-PAGE | Gluconobacter japonicus |
60000 | - |
1 * 60000 + 1 * 49000 + 1 * 16000, calculated from amino acid sequence | Gluconobacter japonicus |
68000 | - |
1 * 68000 + 1 * 51000 + 1 * 18000, SDS-PAGE | Gluconobacter japonicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gluconobacter japonicus | - |
formerly Gluconobacter industrius strain IFO3260 | - |
Gluconobacter japonicus NBRC3260 | - |
formerly Gluconobacter industrius strain IFO3260 | - |
Purification (Comment) | Organism |
---|---|
DEAE-Sepharose column chromatography | Gluconobacter japonicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose + 2,3-dimethoxy-5-farnesyl-1,4-benzoquinone | - |
Gluconobacter japonicus | 5-dehydro-D-fructose + 2,3-dimethoxy-5-farnesyl-1,4-benzoquinol | - |
? | |
D-fructose + 2,3-dimethoxy-5-farnesyl-1,4-benzoquinone | - |
Gluconobacter japonicus NBRC3260 | 5-dehydro-D-fructose + 2,3-dimethoxy-5-farnesyl-1,4-benzoquinol | - |
? | |
D-fructose + 2,3-dimethoxy-5-methyl-1,4-benzoquinone | - |
Gluconobacter japonicus | 5-dehydro-D-fructose + 2,3-dimethoxy-5-methyl-1,4-benzoquinol | - |
? | |
D-fructose + 2,3-dimethoxy-5-methyl-1,4-benzoquinone | - |
Gluconobacter japonicus NBRC3260 | 5-dehydro-D-fructose + 2,3-dimethoxy-5-methyl-1,4-benzoquinol | - |
? | |
additional information | cells producing only subunits FdhS and FdhL have no fructose-oxidizing activity, but show significantly high D-fructose:ferricyanide oxidoreductase activity in the soluble fraction of cell extracts, whereas the cells producing the FDH complex show activity in the membrane fraction | Gluconobacter japonicus | ? | - |
? | |
additional information | cells producing only subunits FdhS and FdhL have no fructose-oxidizing activity, but show significantly high D-fructose:ferricyanide oxidoreductase activity in the soluble fraction of cell extracts, whereas the cells producing the FDH complex show activity in the membrane fraction | Gluconobacter japonicus NBRC3260 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotrimer | 1 * 60000 + 1 * 49000 + 1 * 16000, calculated from amino acid sequence | Gluconobacter japonicus |
heterotrimer | 1 * 68000 + 1 * 51000 + 1 * 18000, SDS-PAGE | Gluconobacter japonicus |
Synonyms | Comment | Organism |
---|---|---|
FDH | - |
Gluconobacter japonicus |
FdhL | large subunit | Gluconobacter japonicus |
FdhS | small subunit | Gluconobacter japonicus |
fdhSCL | gene name of the flavoprotein-cytochrome c complex fructose dehydrogenase | Gluconobacter japonicus |
fructose dehydrogenase | - |
Gluconobacter japonicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
- |
Gluconobacter japonicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome c | the cytochrome c subunit is responsible not only for membrane anchoring but also for ubiquinone reduction | Gluconobacter japonicus | |
heme | the enzyme has three heme C moieties | Gluconobacter japonicus |