Cloned (Comment) | Organism |
---|---|
expression of isozyme PQQGDH-B with and without an polyarginine tail in Escherichia coli strain PP2418, and of mutant H168Q | Acinetobacter calcoaceticus |
Protein Variants | Comment | Organism |
---|---|---|
H168Q | site-directed mutagenesis, inactive mutant, a heterodimeric chimeric enzyme consisiting of 1 wild-type subunit and 1 mutant subunit shows decreased activity and a substrate specificity similar to the wild-type enzyme | Acinetobacter calcoaceticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics, cooperativity in the recombinant chimeric mutant enzyme which possesses only 1 active subunit derived from the wild-type enzyme | Acinetobacter calcoaceticus | |
2 | 3 | D-glucose | recombinant Arg-tagged enzyme, pH 7.0 | Acinetobacter calcoaceticus | |
2 | 3 | 3-O-methyl-D-glucose | recombinant Arg-tagged enzyme, pH 7.0 | Acinetobacter calcoaceticus | |
8 | - |
D-galactose | recombinant Arg-tagged enzyme, pH 7.0 | Acinetobacter calcoaceticus | |
9 | - |
D-galactose | recombinant chimeric mutant enzyme, pH 7.0 | Acinetobacter calcoaceticus | |
13 | - |
maltose | recombinant chimeric mutant enzyme, pH 7.0 | Acinetobacter calcoaceticus | |
14 | - |
maltose | recombinant Arg-tagged enzyme, pH 7.0 | Acinetobacter calcoaceticus | |
20 | - |
lactose | recombinant Arg-tagged enzyme, and recombinant chimeric mutant enzyme, pH 7.0 | Acinetobacter calcoaceticus | |
22 | - |
D-glucose | recombinant chimeric mutant enzyme, pH 7.0 | Acinetobacter calcoaceticus | |
22 | - |
3-O-methyl-D-glucose | recombinant chimeric mutant enzyme, pH 7.0 | Acinetobacter calcoaceticus | |
34 | - |
allose | recombinant Arg-tagged enzyme, pH 7.0 | Acinetobacter calcoaceticus | |
36 | - |
allose | recombinant chimeric mutant enzyme, pH 7.0 | Acinetobacter calcoaceticus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | isozyme PQQGDH-B | Acinetobacter calcoaceticus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Acinetobacter calcoaceticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
2 * 50000, SDS-PAGE | Acinetobacter calcoaceticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter calcoaceticus | - |
isozyme PQQGDH-B | - |
Renatured (Comment) | Organism |
---|---|
protein dissociation and redimerization of recombinant wild-type and Arg-tagged enzyme, and the mutant H168Q, overview | Acinetobacter calcoaceticus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2642 | - |
purified recombinant mutant chimeric isozyme PQQGDH-B | Acinetobacter calcoaceticus |
4512 | - |
purified recombinant Arg-tagged isozyme PQQGDH-B | Acinetobacter calcoaceticus |
4610 | - |
purified recombinant wild-type isozyme PQQGDH-B | Acinetobacter calcoaceticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-O-methyl-D-glucose + 2,6-dichlorophenolindolphenol | - |
Acinetobacter calcoaceticus | 3-O-methyl-D-glucono-1,5-lactone + ? | - |
? | |
allose + 2,6-dichlorophenolindolphenol | - |
Acinetobacter calcoaceticus | ? | - |
? | |
D-galactose + 2,6-dichlorophenolindolphenol | - |
Acinetobacter calcoaceticus | D-galactono-1,5-lactone + ? | - |
? | |
D-glucose + 2,6-dichlorophenolindolphenol | - |
Acinetobacter calcoaceticus | D-glucono-1,5-lactone + ? | - |
? | |
D-glucose + ubiquinone | - |
Acinetobacter calcoaceticus | D-glucono-1,5-lactone + ubiquinol | - |
? | |
lactose + 2,6-dichlorophenolindolphenol | - |
Acinetobacter calcoaceticus | ? | - |
? | |
maltose + 2,6-dichlorophenolindolphenol | - |
Acinetobacter calcoaceticus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 50000, SDS-PAGE | Acinetobacter calcoaceticus |
Synonyms | Comment | Organism |
---|---|---|
glucose dehydrogenase | - |
Acinetobacter calcoaceticus |
PQQGDH-B | - |
Acinetobacter calcoaceticus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3778 | - |
D-glucose | recombinant Arg-tagged enzyme, pH 7.0 | Acinetobacter calcoaceticus | |
4424 | - |
D-glucose | recombinant chimeric mutant enzyme, pH 7.0 | Acinetobacter calcoaceticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Acinetobacter calcoaceticus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyrroloquinoline quinone | i.e. PQQ, dependent on | Acinetobacter calcoaceticus |