KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4 | - |
(R)-solketal | pH 7.5, presence of CaCl2 | Comamonas testosteroni | |
81 | - |
(R)-solketal aldehyde | pH 7.5, presence of CaCl2 | Comamonas testosteroni | |
182 | - |
(S)-solketal aldehyde | pH 7.5, presence of CaCl2 | Comamonas testosteroni | |
197 | - |
Ethanal | pH 7.5, presence of CaCl2 | Comamonas testosteroni | |
211 | - |
ethanol | pH 7.5, presence of CaCl2 | Comamonas testosteroni | |
302 | - |
(R)-3-bromo-2-methyl-1-propanol | pH 7.5, presence of CaCl2 | Comamonas testosteroni |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Comamonas testosteroni | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-3-bromo-2-methyl-1-propanol + ferricyanide | - |
Comamonas testosteroni | (R)-3-bromo-2-methyl-propanal + ferrocyanide | - |
r | |
(R)-solketal + 2 ferricyanide | (R)-enantiomer is prefered, enantiomeric ratio is 30 | Comamonas testosteroni | (R)-solketal aldehyde + 2 ferrocyanide + 2 H+ | - |
r | |
(R)-solketal aldehyde + ferrocyanide | - |
Comamonas testosteroni | (R)-solketal + ferricyanide | - |
r | |
(R)-solketal aldehyde + ferrocyanide | (R)-enantiomer is prefered, enantiomeric ratio is 2.8 for initial rate measurement | Comamonas testosteroni | (R)-solketal + ferricyanide | - |
r | |
(S)-solketal + 2 ferricyanide | the second step in (S)-solketal conversion is much faster than the first one and that opposite enantiomeric preferences exist for the alcohol and the aldehyde substrates. (R)-enantiomer is prefered, enantiomeric ratio is 30 for initial rate measurement, 117 for progress curve analysis | Comamonas testosteroni | (S)-solketal aldehyde + 2 ferrocyanide + 2 H+ | - |
r | |
ethanal + ferrocyanide | - |
Comamonas testosteroni | ethanol + ferricyanide | - |
r | |
ethanol + ferricyanide | - |
Comamonas testosteroni | ethanal + ferrocyanide | - |
r |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.18 | - |
(S)-solketal | pH 7.5, presence of CaCl2 | Comamonas testosteroni | |
2.12 | - |
(R)-solketal | pH 7.5, presence of CaCl2 | Comamonas testosteroni | |
8.3 | - |
(R)-solketal aldehyde | pH 7.5, presence of CaCl2 | Comamonas testosteroni | |
17.1 | - |
(S)-solketal aldehyde | pH 7.5, presence of CaCl2 | Comamonas testosteroni | |
17.9 | - |
ethanol | pH 7.5, presence of CaCl2 | Comamonas testosteroni | |
19.9 | - |
Ethanal | pH 7.5, presence of CaCl2 | Comamonas testosteroni | |
20.3 | - |
(R)-3-bromo-2-methyl-1-propanol | pH 7.5, presence of CaCl2 | Comamonas testosteroni |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
67 | - |
(R)-3-bromo-2-methyl-1-propanol | pH 7.5, presence of CaCl2 | Comamonas testosteroni | |
85 | - |
ethanol | pH 7.5, presence of CaCl2 | Comamonas testosteroni | |
94 | - |
(S)-solketal aldehyde | pH 7.5, presence of CaCl2 | Comamonas testosteroni | |
101 | - |
Ethanal | pH 7.5, presence of CaCl2 | Comamonas testosteroni | |
102 | - |
(R)-solketal aldehyde | pH 7.5, presence of CaCl2 | Comamonas testosteroni | |
517 | - |
(R)-solketal | pH 7.5, presence of CaCl2 | Comamonas testosteroni |