Description of the kinetic mechanism and the enantioselectivity of quinohaemoprotein ethanol dehydrogenase from Comamonas testosteroni in the oxidation of alcohols and aldehydes
Geerlof, A.; Rakels, J.J.L.; Straathof, A.J.J.; Heijnen, J.J.; Jongejan, J.A.; Duine, J.A.; Eur. J. Biochem. 226, 537-546 (1994) 
Data extracted from this reference:
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4
-
(R)-solketal
pH 7.5, presence of CaCl2
Comamonas testosteroni
81
-
(R)-solketal aldehyde
pH 7.5, presence of CaCl2
Comamonas testosteroni
182
-
(S)-solketal aldehyde
pH 7.5, presence of CaCl2
Comamonas testosteroni
197
-
Ethanal
pH 7.5, presence of CaCl2
Comamonas testosteroni
211
-
ethanol
pH 7.5, presence of CaCl2
Comamonas testosteroni
302
-
(R)-3-bromo-2-methyl-1-propanol
pH 7.5, presence of CaCl2
Comamonas testosteroni
Organism
Organism
UniProt
Commentary
Textmining
Comamonas testosteroni
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(R)-3-bromo-2-methyl-1-propanol + ferricyanide
-
703526
Comamonas testosteroni
(R)-3-bromo-2-methyl-propanal + ferrocyanide
-
-
-
r
(R)-solketal + 2 ferricyanide
(R)-enantiomer is prefered, enantiomeric ratio is 30
703526
Comamonas testosteroni
(R)-solketal aldehyde + 2 ferrocyanide + 2 H+
-
-
-
r
(R)-solketal aldehyde + ferrocyanide
-
703526
Comamonas testosteroni
(R)-solketal + ferricyanide
-
-
-
r
(R)-solketal aldehyde + ferrocyanide
(R)-enantiomer is prefered, enantiomeric ratio is 2.8 for initial rate measurement
703526
Comamonas testosteroni
(R)-solketal + ferricyanide
-
-
-
r
(S)-solketal + 2 ferricyanide
the second step in (S)-solketal conversion is much faster than the first one and that opposite enantiomeric preferences exist for the alcohol and the aldehyde substrates. (R)-enantiomer is prefered, enantiomeric ratio is 30 for initial rate measurement, 117 for progress curve analysis
703526
Comamonas testosteroni
(S)-solketal aldehyde + 2 ferrocyanide + 2 H+
-
-
-
r
ethanal + ferrocyanide
-
703526
Comamonas testosteroni
ethanol + ferricyanide
-
-
-
r
ethanol + ferricyanide
-
703526
Comamonas testosteroni
ethanal + ferrocyanide
-
-
-
r
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.18
-
(S)-solketal
pH 7.5, presence of CaCl2
Comamonas testosteroni
2.12
-
(R)-solketal
pH 7.5, presence of CaCl2
Comamonas testosteroni
8.3
-
(R)-solketal aldehyde
pH 7.5, presence of CaCl2
Comamonas testosteroni
17.1
-
(S)-solketal aldehyde
pH 7.5, presence of CaCl2
Comamonas testosteroni
17.9
-
ethanol
pH 7.5, presence of CaCl2
Comamonas testosteroni
19.9
-
Ethanal
pH 7.5, presence of CaCl2
Comamonas testosteroni
20.3
-
(R)-3-bromo-2-methyl-1-propanol
pH 7.5, presence of CaCl2
Comamonas testosteroni
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4
-
(R)-solketal
pH 7.5, presence of CaCl2
Comamonas testosteroni
81
-
(R)-solketal aldehyde
pH 7.5, presence of CaCl2
Comamonas testosteroni
182
-
(S)-solketal aldehyde
pH 7.5, presence of CaCl2
Comamonas testosteroni
197
-
Ethanal
pH 7.5, presence of CaCl2
Comamonas testosteroni
211
-
ethanol
pH 7.5, presence of CaCl2
Comamonas testosteroni
302
-
(R)-3-bromo-2-methyl-1-propanol
pH 7.5, presence of CaCl2
Comamonas testosteroni
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(R)-3-bromo-2-methyl-1-propanol + ferricyanide
-
703526
Comamonas testosteroni
(R)-3-bromo-2-methyl-propanal + ferrocyanide
-
-
-
r
(R)-solketal + 2 ferricyanide
(R)-enantiomer is prefered, enantiomeric ratio is 30
703526
Comamonas testosteroni
(R)-solketal aldehyde + 2 ferrocyanide + 2 H+
-
-
-
r
(R)-solketal aldehyde + ferrocyanide
-
703526
Comamonas testosteroni
(R)-solketal + ferricyanide
-
-
-
r
(R)-solketal aldehyde + ferrocyanide
(R)-enantiomer is prefered, enantiomeric ratio is 2.8 for initial rate measurement
703526
Comamonas testosteroni
(R)-solketal + ferricyanide
-
-
-
r
(S)-solketal + 2 ferricyanide
the second step in (S)-solketal conversion is much faster than the first one and that opposite enantiomeric preferences exist for the alcohol and the aldehyde substrates. (R)-enantiomer is prefered, enantiomeric ratio is 30 for initial rate measurement, 117 for progress curve analysis
703526
Comamonas testosteroni
(S)-solketal aldehyde + 2 ferrocyanide + 2 H+
-
-
-
r
ethanal + ferrocyanide
-
703526
Comamonas testosteroni
ethanol + ferricyanide
-
-
-
r
ethanol + ferricyanide
-
703526
Comamonas testosteroni
ethanal + ferrocyanide
-
-
-
r
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.18
-
(S)-solketal
pH 7.5, presence of CaCl2
Comamonas testosteroni
2.12
-
(R)-solketal
pH 7.5, presence of CaCl2
Comamonas testosteroni
8.3
-
(R)-solketal aldehyde
pH 7.5, presence of CaCl2
Comamonas testosteroni
17.1
-
(S)-solketal aldehyde
pH 7.5, presence of CaCl2
Comamonas testosteroni
17.9
-
ethanol
pH 7.5, presence of CaCl2
Comamonas testosteroni
19.9
-
Ethanal
pH 7.5, presence of CaCl2
Comamonas testosteroni
20.3
-
(R)-3-bromo-2-methyl-1-propanol
pH 7.5, presence of CaCl2
Comamonas testosteroni
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
67
-
(R)-3-bromo-2-methyl-1-propanol
pH 7.5, presence of CaCl2
Comamonas testosteroni
85
-
ethanol
pH 7.5, presence of CaCl2
Comamonas testosteroni
94
-
(S)-solketal aldehyde
pH 7.5, presence of CaCl2
Comamonas testosteroni
101
-
Ethanal
pH 7.5, presence of CaCl2
Comamonas testosteroni
102
-
(R)-solketal aldehyde
pH 7.5, presence of CaCl2
Comamonas testosteroni
517
-
(R)-solketal
pH 7.5, presence of CaCl2
Comamonas testosteroni
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
67
-
(R)-3-bromo-2-methyl-1-propanol
pH 7.5, presence of CaCl2
Comamonas testosteroni
85
-
ethanol
pH 7.5, presence of CaCl2
Comamonas testosteroni
94
-
(S)-solketal aldehyde
pH 7.5, presence of CaCl2
Comamonas testosteroni
101
-
Ethanal
pH 7.5, presence of CaCl2
Comamonas testosteroni
102
-
(R)-solketal aldehyde
pH 7.5, presence of CaCl2
Comamonas testosteroni
517
-
(R)-solketal
pH 7.5, presence of CaCl2
Comamonas testosteroni
Other publictions for EC 1.1.9.1
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
654868
Jongejan
Deuterium isotope effect on en ...
Comamonas testosteroni
Biochim. Biophys. Acta
1647
297-302
2003
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1
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2
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1
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2
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1
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656080
Oubrie
Crystal structure of quinohemo ...
Comamonas testosteroni
J. Biol. Chem.
277
3727-3732
2002
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1
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1
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1
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678768
Toyama
Azurin involved in alcohol oxi ...
Pseudomonas putida
Biosci. Biotechnol. Biochem.
65
1617-1626
2001
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1
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2
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1
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701480
Oubrie
Crystallization of quinohemopr ...
Comamonas testosteroni
Acta Crystallogr. Sect. D
D57
1732-1734
2001
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1
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1
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1
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1
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1
1
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701774
Somers
-
Enantioselective oxidation of ...
Comamonas testosteroni
Appl. Biochem. Biotechnol.
75
151-161
1999
-
1
-
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59
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1
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36
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1
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59
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36
-
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705187
Stigter
-
Enantioselective oxidation of ...
Comamonas testosteroni
J. Mol. Catal. B
2
291-297
1997
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21
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1
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22
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21
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22
-
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-
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-
-
-
-
389956
De Jong
Characterization of the intera ...
Comamonas testosteroni
Biochemistry
34
9451-9458
1995
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1
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2
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2
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389960
De Jong
Quinohemoprotein ethanol dehyd ...
Comamonas testosteroni
Eur. J. Biochem.
230
899-905
1995
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1
2
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1
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1
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1
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1
1
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2
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2
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1
2
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1
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1
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1
1
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-
687362
Toyama
Three distinct quinoprotein al ...
Pseudomonas putida
J. Bacteriol.
177
2442-2450
1995
-
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7
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2
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1
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1
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26
1
2
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2
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2
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7
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2
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1
-
26
1
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-
-
-
-
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1
1
-
-
-
703526
Geerlof
Description of the kinetic mec ...
Comamonas testosteroni
Eur. J. Biochem.
226
537-546
1994
-
-
-
-
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-
-
6
-
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1
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7
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7
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6
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7
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7
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6
6
389953
Groen
Quinohaemoprotein alcohol dehy ...
Comamonas testosteroni
Biochem. J.
234
611-615
1986
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12
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1
2
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1
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2
15
1
1
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1
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2
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2
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12
-
1
2
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2
15
1
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1
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