Data extracted from this reference:
KM Value [mM]
4
(R)-solketal
pH 7.5, presence of CaCl2
Comamonas testosteroni
81
(R)-solketal aldehyde
pH 7.5, presence of CaCl2
Comamonas testosteroni
182
(S)-solketal aldehyde
pH 7.5, presence of CaCl2
Comamonas testosteroni
197
Ethanal
pH 7.5, presence of CaCl2
Comamonas testosteroni
211
ethanol
pH 7.5, presence of CaCl2
Comamonas testosteroni
302
(R)-3-bromo-2-methyl-1-propanol
pH 7.5, presence of CaCl2
Comamonas testosteroni
Organism
Substrates and Products (Substrate)
(R)-3-bromo-2-methyl-1-propanol + ferricyanide
703526
Comamonas testosteroni
(R)-3-bromo-2-methyl-propanal + ferrocyanide
r
(R)-solketal + 2 ferricyanide
(R)-enantiomer is prefered, enantiomeric ratio is 30
703526
Comamonas testosteroni
(R)-solketal aldehyde + 2 ferrocyanide + 2 H+
r
(R)-solketal aldehyde + ferrocyanide
703526
Comamonas testosteroni
(R)-solketal + ferricyanide
r
(R)-solketal aldehyde + ferrocyanide
(R)-enantiomer is prefered, enantiomeric ratio is 2.8 for initial rate measurement
703526
Comamonas testosteroni
(R)-solketal + ferricyanide
r
(S)-solketal + 2 ferricyanide
the second step in (S)-solketal conversion is much faster than the first one and that opposite enantiomeric preferences exist for the alcohol and the aldehyde substrates. (R)-enantiomer is prefered, enantiomeric ratio is 30 for initial rate measurement, 117 for progress curve analysis
703526
Comamonas testosteroni
(S)-solketal aldehyde + 2 ferrocyanide + 2 H+
r
ethanal + ferrocyanide
703526
Comamonas testosteroni
ethanol + ferricyanide
r
ethanol + ferricyanide
703526
Comamonas testosteroni
ethanal + ferrocyanide
r
Turnover Number [1/s]
0.18
(S)-solketal
pH 7.5, presence of CaCl2
Comamonas testosteroni
2.12
(R)-solketal
pH 7.5, presence of CaCl2
Comamonas testosteroni
8.3
(R)-solketal aldehyde
pH 7.5, presence of CaCl2
Comamonas testosteroni
17.1
(S)-solketal aldehyde
pH 7.5, presence of CaCl2
Comamonas testosteroni
17.9
ethanol
pH 7.5, presence of CaCl2
Comamonas testosteroni
19.9
Ethanal
pH 7.5, presence of CaCl2
Comamonas testosteroni
20.3
(R)-3-bromo-2-methyl-1-propanol
pH 7.5, presence of CaCl2
Comamonas testosteroni
KM Value [mM] (protein specific)
4
(R)-solketal
pH 7.5, presence of CaCl2
Comamonas testosteroni
81
(R)-solketal aldehyde
pH 7.5, presence of CaCl2
Comamonas testosteroni
182
(S)-solketal aldehyde
pH 7.5, presence of CaCl2
Comamonas testosteroni
197
Ethanal
pH 7.5, presence of CaCl2
Comamonas testosteroni
211
ethanol
pH 7.5, presence of CaCl2
Comamonas testosteroni
302
(R)-3-bromo-2-methyl-1-propanol
pH 7.5, presence of CaCl2
Comamonas testosteroni
Substrates and Products (Substrate) (protein specific)
(R)-3-bromo-2-methyl-1-propanol + ferricyanide
703526
Comamonas testosteroni
(R)-3-bromo-2-methyl-propanal + ferrocyanide
r
(R)-solketal + 2 ferricyanide
(R)-enantiomer is prefered, enantiomeric ratio is 30
703526
Comamonas testosteroni
(R)-solketal aldehyde + 2 ferrocyanide + 2 H+
r
(R)-solketal aldehyde + ferrocyanide
703526
Comamonas testosteroni
(R)-solketal + ferricyanide
r
(R)-solketal aldehyde + ferrocyanide
(R)-enantiomer is prefered, enantiomeric ratio is 2.8 for initial rate measurement
703526
Comamonas testosteroni
(R)-solketal + ferricyanide
r
(S)-solketal + 2 ferricyanide
the second step in (S)-solketal conversion is much faster than the first one and that opposite enantiomeric preferences exist for the alcohol and the aldehyde substrates. (R)-enantiomer is prefered, enantiomeric ratio is 30 for initial rate measurement, 117 for progress curve analysis
703526
Comamonas testosteroni
(S)-solketal aldehyde + 2 ferrocyanide + 2 H+
r
ethanal + ferrocyanide
703526
Comamonas testosteroni
ethanol + ferricyanide
r
ethanol + ferricyanide
703526
Comamonas testosteroni
ethanal + ferrocyanide
r
Turnover Number [1/s] (protein specific)
0.18
(S)-solketal
pH 7.5, presence of CaCl2
Comamonas testosteroni
2.12
(R)-solketal
pH 7.5, presence of CaCl2
Comamonas testosteroni
8.3
(R)-solketal aldehyde
pH 7.5, presence of CaCl2
Comamonas testosteroni
17.1
(S)-solketal aldehyde
pH 7.5, presence of CaCl2
Comamonas testosteroni
17.9
ethanol
pH 7.5, presence of CaCl2
Comamonas testosteroni
19.9
Ethanal
pH 7.5, presence of CaCl2
Comamonas testosteroni
20.3
(R)-3-bromo-2-methyl-1-propanol
pH 7.5, presence of CaCl2
Comamonas testosteroni
KCat/KM [mM/s]
67
(R)-3-bromo-2-methyl-1-propanol
pH 7.5, presence of CaCl2
Comamonas testosteroni
85
ethanol
pH 7.5, presence of CaCl2
Comamonas testosteroni
94
(S)-solketal aldehyde
pH 7.5, presence of CaCl2
Comamonas testosteroni
101
Ethanal
pH 7.5, presence of CaCl2
Comamonas testosteroni
102
(R)-solketal aldehyde
pH 7.5, presence of CaCl2
Comamonas testosteroni
517
(R)-solketal
pH 7.5, presence of CaCl2
Comamonas testosteroni
KCat/KM [mM/s] (protein specific)
67
(R)-3-bromo-2-methyl-1-propanol
pH 7.5, presence of CaCl2
Comamonas testosteroni
85
ethanol
pH 7.5, presence of CaCl2
Comamonas testosteroni
94
(S)-solketal aldehyde
pH 7.5, presence of CaCl2
Comamonas testosteroni
101
Ethanal
pH 7.5, presence of CaCl2
Comamonas testosteroni
102
(R)-solketal aldehyde
pH 7.5, presence of CaCl2
Comamonas testosteroni
517
(R)-solketal
pH 7.5, presence of CaCl2
Comamonas testosteroni
Other publictions for EC 1.1.9.1
654868
Jongejan
Deuterium isotope effect on en ...
Comamonas testosteroni
Biochim. Biophys. Acta
1647
297-302
2003
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656080
Oubrie
Crystal structure of quinohemo ...
Comamonas testosteroni
J. Biol. Chem.
277
3727-3732
2002
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678768
Toyama
Azurin involved in alcohol oxi ...
Pseudomonas putida
Biosci. Biotechnol. Biochem.
65
1617-1626
2001
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1
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701480
Oubrie
Crystallization of quinohemopr ...
Comamonas testosteroni
Acta Crystallogr. Sect. D
D57
1732-1734
2001
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1
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701774
Somers
-
Enantioselective oxidation of ...
Comamonas testosteroni
Appl. Biochem. Biotechnol.
75
151-161
1999
-
1
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-
59
-
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1
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36
-
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1
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59
-
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36
-
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-
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-
-
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-
-
-
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705187
Stigter
-
Enantioselective oxidation of ...
Comamonas testosteroni
J. Mol. Catal. B
2
291-297
1997
-
-
-
-
-
-
-
21
-
-
-
-
-
1
-
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-
-
-
-
-
-
22
-
-
-
-
-
-
-
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-
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-
-
-
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-
-
-
-
-
-
-
-
-
21
-
-
-
-
-
-
-
-
-
-
-
-
22
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389956
De Jong
Characterization of the intera ...
Comamonas testosteroni
Biochemistry
34
9451-9458
1995
-
-
-
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-
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1
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2
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2
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389960
De Jong
Quinohemoprotein ethanol dehyd ...
Comamonas testosteroni
Eur. J. Biochem.
230
899-905
1995
-
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-
-
-
-
1
2
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1
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1
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1
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1
1
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2
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2
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1
2
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-
1
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1
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1
1
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-
-
-
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-
687362
Toyama
Three distinct quinoprotein al ...
Pseudomonas putida
J. Bacteriol.
177
2442-2450
1995
-
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7
-
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2
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1
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-
-
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-
1
-
26
1
2
-
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-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
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-
7
-
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2
-
-
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-
1
-
26
1
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-
-
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1
1
-
-
-
703526
Geerlof
Description of the kinetic mec ...
Comamonas testosteroni
Eur. J. Biochem.
226
537-546
1994
-
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6
-
-
-
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-
1
-
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-
-
-
-
-
-
7
-
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-
-
-
7
-
-
-
-
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-
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-
-
-
-
-
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-
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6
-
-
-
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-
-
-
-
-
-
-
7
-
-
-
-
7
-
-
-
-
-
-
-
-
6
6
389953
Groen
Quinohaemoprotein alcohol dehy ...
Comamonas testosteroni
Biochem. J.
234
611-615
1986
-
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-
-
-
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-
12
-
1
2
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-
1
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-
-
-
-
-
-
2
15
1
1
-
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-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
12
-
1
2
-
-
-
-
-
-
-
-
2
15
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-