Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Top10 cells | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
E109Q | the mutant has 60fold decreased catalytic efficiency compared to the wild type enzyme | Mycobacterium tuberculosis |
H40A | the mutant has 1000fold decreased catalytic efficiency compared to the wild type enzyme | Mycobacterium tuberculosis |
W44A | the mutant has 2000fold decreased catalytic efficiency compared to the wild type enzyme | Mycobacterium tuberculosis |
W44F | the mutant has 2fold increased catalytic efficiency compared to the wild type enzyme | Mycobacterium tuberculosis |
W44Y | the mutant has 34fold decreased catalytic efficiency compared to the wild type enzyme | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0009 | - |
oxidized coenzyme F420 | mutant enzyme W44A, at pH 7.0 and 22°C | Mycobacterium tuberculosis | |
0.0026 | - |
oxidized coenzyme F420 | wild type enzyme, at pH 7.0 and 22°C | Mycobacterium tuberculosis | |
0.0042 | - |
oxidized coenzyme F420 | mutant enzyme W44Y, at pH 7.0 and 22°C | Mycobacterium tuberculosis | |
0.0054 | - |
oxidized coenzyme F420 | mutant enzyme W44F, at pH 7.0 and 22°C | Mycobacterium tuberculosis | |
0.0056 | - |
oxidized coenzyme F420 | mutant enzyme E109Q, at pH 7.0 and 22°C | Mycobacterium tuberculosis | |
0.008 | - |
oxidized coenzyme F420 | mutant enzyme H40A, at pH 7.0 and 22°C | Mycobacterium tuberculosis | |
0.022 | - |
D-glucose 6-phosphate | wild type enzyme, at pH 7.0 and 22°C | Mycobacterium tuberculosis | |
0.027 | - |
D-glucose 6-phosphate | mutant enzyme H40A, at pH 7.0 and 22°C | Mycobacterium tuberculosis | |
0.041 | - |
D-glucose 6-phosphate | mutant enzyme E109Q, at pH 7.0 and 22°C | Mycobacterium tuberculosis | |
0.17 | - |
D-glucose 6-phosphate | mutant enzyme W44Y, at pH 7.0 and 22°C | Mycobacterium tuberculosis | |
0.28 | - |
D-glucose 6-phosphate | mutant enzyme W44A, at pH 7.0 and 22°C | Mycobacterium tuberculosis | |
0.34 | - |
D-glucose 6-phosphate | mutant enzyme W44F, at pH 7.0 and 22°C | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate + oxidized coenzyme F420 | Mycobacterium tuberculosis | - |
6-phospho-D-glucono-1,5-lactone + reduced coenzyme F420 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | Q0RVH7 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Sephacryl 200 gel filtration | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate + oxidized coenzyme F420 | - |
Mycobacterium tuberculosis | 6-phospho-D-glucono-1,5-lactone + reduced coenzyme F420 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
F420-dependent glucose-6-phosphate dehydrogenase | - |
Mycobacterium tuberculosis |
FGD | - |
Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
oxidized coenzyme F420 | mutant enzyme W44A, at pH 7.0 and 22°C | Mycobacterium tuberculosis | |
0.0012 | - |
D-glucose 6-phosphate | mutant enzyme W44A, at pH 7.0 and 22°C | Mycobacterium tuberculosis | |
0.0016 | - |
D-glucose 6-phosphate | mutant enzyme H40A, at pH 7.0 and 22°C | Mycobacterium tuberculosis | |
0.002 | - |
oxidized coenzyme F420 | mutant enzyme H40A, at pH 7.0 and 22°C | Mycobacterium tuberculosis | |
0.02 | - |
oxidized coenzyme F420 | mutant enzyme E109Q, at pH 7.0 and 22°C | Mycobacterium tuberculosis | |
0.023 | - |
D-glucose 6-phosphate | mutant enzyme E109Q, at pH 7.0 and 22°C | Mycobacterium tuberculosis | |
0.041 | - |
D-glucose 6-phosphate | mutant enzyme W44Y, at pH 7.0 and 22°C | Mycobacterium tuberculosis | |
0.06 | - |
oxidized coenzyme F420 | mutant enzyme W44Y, at pH 7.0 and 22°C | Mycobacterium tuberculosis | |
1.4 | - |
D-glucose 6-phosphate | wild type enzyme, at pH 7.0 and 22°C | Mycobacterium tuberculosis | |
1.4 | - |
oxidized coenzyme F420 | wild type enzyme, at pH 7.0 and 22°C | Mycobacterium tuberculosis | |
3.2 | - |
oxidized coenzyme F420 | mutant enzyme W44F, at pH 7.0 and 22°C | Mycobacterium tuberculosis | |
3.5 | - |
D-glucose 6-phosphate | mutant enzyme W44F, at pH 7.0 and 22°C | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
coenzyme F420 | - |
Mycobacterium tuberculosis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
D-glucose 6-phosphate | mutant enzyme W44A, at pH 7.0 and 22°C | Mycobacterium tuberculosis | |
0.06 | - |
D-glucose 6-phosphate | mutant enzyme H40A, at pH 7.0 and 22°C | Mycobacterium tuberculosis | |
0.1 | - |
D-glucose 6-phosphate | mutant enzyme E109Q, at pH 7.0 and 22°C | Mycobacterium tuberculosis | |
0.2 | - |
D-glucose 6-phosphate | mutant enzyme W44Y, at pH 7.0 and 22°C | Mycobacterium tuberculosis | |
0.4 | - |
oxidized coenzyme F420 | mutant enzyme H40A, at pH 7.0 and 22°C | Mycobacterium tuberculosis | |
1 | - |
oxidized coenzyme F420 | mutant enzyme W44A, at pH 7.0 and 22°C | Mycobacterium tuberculosis | |
4 | - |
oxidized coenzyme F420 | mutant enzyme E109Q, at pH 7.0 and 22°C | Mycobacterium tuberculosis | |
10 | - |
D-glucose 6-phosphate | mutant enzyme W44F, at pH 7.0 and 22°C | Mycobacterium tuberculosis | |
20 | - |
oxidized coenzyme F420 | mutant enzyme W44Y, at pH 7.0 and 22°C | Mycobacterium tuberculosis | |
60 | - |
D-glucose 6-phosphate | wild type enzyme, at pH 7.0 and 22°C | Mycobacterium tuberculosis | |
600 | - |
oxidized coenzyme F420 | wild type enzyme, at pH 7.0 and 22°C | Mycobacterium tuberculosis | |
600 | - |
oxidized coenzyme F420 | mutant enzyme W44F, at pH 7.0 and 22°C | Mycobacterium tuberculosis |