BRENDA - Enzyme Database show
show all sequences of 1.1.99.20

Biodegradation of polyethylene glycol by symbiotic mixed culture (obligate mutualism)

Kawai, F.; Yamanaka, H.; Arch. Microbiol. 146, 125-129 (1986)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
phenazine methosulfate
mediator to dichlorophenolindophenol reduction
Flavobacterium sp.
phenazine methosulfate
mediator to dichlorophenolindophenol reduction
Pseudomonas sp.
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Flavobacterium sp.
-
synergistic mixed culture with Pseudomonas sp.
-
Pseudomonas sp.
-
synergistic mixed culture with Flavobacterium sp.
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
0.001
-
PEG-6000
Flavobacterium sp.
0.003
-
PEG-400
Flavobacterium sp.
0.004
-
valeraldehyde, mixed culture
Flavobacterium sp.
0.004
-
valeraldehyde, mixed culture
Pseudomonas sp.
0.023
-
PEG 6000, mixed culture
Flavobacterium sp.
0.023
-
PEG 6000, mixed culture
Pseudomonas sp.
0.029
-
valeraldehyde
Flavobacterium sp.
0.156
-
PEG-400, mixed culture
Flavobacterium sp.
0.156
-
PEG-400, mixed culture
Pseudomonas sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
polyethylene glycol + acceptor
-
389909
Pseudomonas sp.
?
-
-
-
?
polyethylene glycol + acceptor
-
389909
Flavobacterium sp.
?
-
-
-
?
primary alcohol + acceptor
2,6-dichlorophenolindophenol is the acceptor in vitro
389909
Pseudomonas sp.
aldehyde + reduced acceptor
-
389909
Pseudomonas sp.
?
primary alcohol + acceptor
2,6-dichlorophenolindophenol is the acceptor in vitro
389909
Flavobacterium sp.
aldehyde + reduced acceptor
-
389909
Flavobacterium sp.
?
Cofactor
Cofactor
Commentary
Organism
Structure
2,6-dichlorophenolindophenol
electron acceptor
Flavobacterium sp.
2,6-dichlorophenolindophenol
electron acceptor
Pseudomonas sp.
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
phenazine methosulfate
mediator to dichlorophenolindophenol reduction
Flavobacterium sp.
phenazine methosulfate
mediator to dichlorophenolindophenol reduction
Pseudomonas sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
2,6-dichlorophenolindophenol
electron acceptor
Flavobacterium sp.
2,6-dichlorophenolindophenol
electron acceptor
Pseudomonas sp.
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
0.001
-
PEG-6000
Flavobacterium sp.
0.003
-
PEG-400
Flavobacterium sp.
0.004
-
valeraldehyde, mixed culture
Flavobacterium sp.
0.004
-
valeraldehyde, mixed culture
Pseudomonas sp.
0.023
-
PEG 6000, mixed culture
Flavobacterium sp.
0.023
-
PEG 6000, mixed culture
Pseudomonas sp.
0.029
-
valeraldehyde
Flavobacterium sp.
0.156
-
PEG-400, mixed culture
Flavobacterium sp.
0.156
-
PEG-400, mixed culture
Pseudomonas sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
polyethylene glycol + acceptor
-
389909
Pseudomonas sp.
?
-
-
-
?
polyethylene glycol + acceptor
-
389909
Flavobacterium sp.
?
-
-
-
?
primary alcohol + acceptor
2,6-dichlorophenolindophenol is the acceptor in vitro
389909
Pseudomonas sp.
aldehyde + reduced acceptor
-
389909
Pseudomonas sp.
?
primary alcohol + acceptor
2,6-dichlorophenolindophenol is the acceptor in vitro
389909
Flavobacterium sp.
aldehyde + reduced acceptor
-
389909
Flavobacterium sp.
?
Other publictions for EC 1.1.99.20
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [░C]
Temperature Range [░C]
Temperature Stability [░C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [░C] (protein specific)
Temperature Range [░C] (protein specific)
Temperature Stability [░C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
725161
Liu
Catalytic mechanism of short e ...
Ensifer sp. AS08
Int. J. Mol. Sci.
14
1218-1231
2013
-
-
1
-
5
-
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6
-
-
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3
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1
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3
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6
-
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1
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1
1
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5
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6
-
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-
-
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-
1
-
-
-
-
3
-
-
-
-
6
-
-
-
-
-
-
-
-
6
6
671546
Hu
Isolation of bacteria able to ...
Sphingopyxis macrogoltabida
Appl. Microbiol. Biotechnol.
73
1407-1413
2007
-
-
-
-
-
-
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-
-
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8
-
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688864
Tani
Structure and conservation of ...
Sphingopyxis macrogoltabida 103, Sphingopyxis macrogoltabida, Sphingopyxis macrogoltabida 203, Sphingopyxis terrae
Microbiology
153
338-346
2007
-
2
2
-
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-
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5
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8
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-
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-
2
2
-
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-
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-
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-
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-
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8
-
-
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-
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-
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-
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-
671461
Ohta
Analysis of amino acid residue ...
Sphingopyxis terrae
Appl. Environ. Microbiol.
72
4388-4396
2006
-
-
-
-
-
-
-
21
-
-
-
-
-
5
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
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21
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389911
Sugimoto
The first step in polyethylene ...
Sphingopyxis terrae
J. Bacteriol.
183
6694-6698
2001
-
-
1
-
-
-
-
10
1
-
2
-
-
7
-
-
1
-
-
-
-
-
12
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
10
1
-
2
-
-
-
-
1
-
-
-
-
12
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389910
Yasuda
-
Polyethylene glycol dehydrogen ...
Rhodoblastus acidophilus, Rhodoblastus acidophilus M402
FEMS Microbiol. Lett.
138
23-28
1996
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
1
-
18
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
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-
1
-
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-
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-
1
-
18
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389907
Yamanaka
-
Inducible or constitutive poly ...
Flavobacterium sp., Pseudomonas sp.
J. Ferment. Bioeng.
67
300-302
1989
4
-
-
-
-
-
2
-
2
3
-
-
-
2
-
-
-
-
-
-
6
-
19
-
-
-
-
-
-
-
-
2
-
-
-
4
-
-
2
-
-
-
-
2
-
-
2
3
-
-
-
-
-
-
-
-
6
-
19
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389908
Yamanaka
-
Purification and characterizat ...
Flavobacterium sp.
J. Ferment. Bioeng.
67
324-330
1989
1
-
-
-
-
-
14
4
1
-
2
-
-
1
-
-
1
-
-
-
2
-
36
1
1
1
2
-
2
1
1
5
-
-
-
1
-
-
5
-
-
-
-
14
-
4
1
-
2
-
-
-
-
1
-
-
2
-
36
1
1
1
2
-
2
1
1
-
-
-
-
-
-
-
389909
Kawai
Biodegradation of polyethylene ...
Flavobacterium sp., Pseudomonas sp.
Arch. Microbiol.
146
125-129
1986
2
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
9
-
4
-
-
-
-
-
-
-
-
2
-
-
-
2
-
-
2
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
9
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389904
Kawai
Identification of the prosthet ...
Flavobacterium sp., Pseudomonas sp.
Agric. Biol. Chem.
49
1071-1076
1985
4
-
-
-
-
-
8
4
2
-
-
-
-
2
-
-
-
-
-
-
-
-
40
-
-
-
-
-
-
-
-
10
-
-
-
4
-
-
10
-
-
-
-
8
-
4
2
-
-
-
-
-
-
-
-
-
-
-
40
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389905
Kawai
-
Identification of reaction pro ...
Flavobacterium sp., Pseudomonas sp.
Agric. Biol. Chem.
47
1669-1671
1983
-
-
-
-
-
-
-
-
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-
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-
2
-
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6
-
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2
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-
2
-
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-
-
-
-
-
-
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6
-
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-
-
-
-
-
-
-
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-
-
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-
389906
Kawai
Purification and characterizat ...
Flavobacterium sp., Pseudomonas sp.
Appl. Environ. Microbiol.
40
701-705
1980
-
-
-
-
-
-
22
4
-
4
4
-
-
3
-
-
2
-
-
-
2
-
28
2
2
2
4
-
2
2
6
2
-
-
-
-
-
-
2
-
-
-
-
22
-
4
-
4
4
-
-
-
-
2
-
-
2
-
28
2
2
2
4
-
2
2
6
-
-
-
-
-
-
-