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show all sequences of 1.1.99.20

Analysis of amino acid residues involved in catalysis of polyethylene glycol dehydrogenase from Sphingopyxis terrae, using three-dimensional molecular modeling-based kinetic characterization of mutants

Ohta, T.; Kawabata, T.; Nishikawa, K.; Tani, A.; Kimbara, K.; Kawai, F.; Appl. Environ. Microbiol. 72, 4388-4396 (2006)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.1
-
benzyl alcohol
mutant C472A
Sphingopyxis terrae
0.11
-
benzyl alcohol
wild type
Sphingopyxis terrae
0.44
-
1-Hexanol
mutant C472A; mutant H467A
Sphingopyxis terrae
0.45
-
1-Hexanol
mutant N511H; wild type
Sphingopyxis terrae
0.81
-
benzyl alkohol
mutant H467A
Sphingopyxis terrae
0.84
-
benzyl alcohol
mutant N511H
Sphingopyxis terrae
1
-
PEG 1000
mutant C382A
Sphingopyxis terrae
1.1
-
benzyl alcohol
mutant C297
Sphingopyxis terrae
1.1
-
PEG 1000
mutant C379A; mutant H467A; wild type
Sphingopyxis terrae
1.2
-
benzyl alcohol
mutant C382A
Sphingopyxis terrae
1.2
-
PEG 1000
mutant C472A; mutant N511H
Sphingopyxis terrae
1.4
-
benzyl alcohol
mutant C379A
Sphingopyxis terrae
1.7
-
1-Hexanol
mutant C379A; mutant C382A
Sphingopyxis terrae
2.3
-
PEG 6000
mutant C472A; mutant N511H
Sphingopyxis terrae
2.4
-
PEG 6000
mutant H467A; wild type
Sphingopyxis terrae
2.5
-
PEG 6000
mutant C297; mutant C379A; mutant C382A
Sphingopyxis terrae
6.7
-
TEG
wild type
Sphingopyxis terrae
6.8
-
TEG
mutant H467A
Sphingopyxis terrae
6.9
-
TEG
mutant C472A; mutant N511H
Sphingopyxis terrae
8.1
-
TEG
mutant C382A
Sphingopyxis terrae
8.3
-
TEG
mutant C379A
Sphingopyxis terrae
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Sphingopyxis terrae
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1-hexanol + acceptor
-
671461
Sphingopyxis terrae
n-hexanal + reduced acceptor
-
-
-
-
benzyl alcohol + acceptor
-
671461
Sphingopyxis terrae
benzaldehyde + reduced acceptor
-
-
-
-
primary alcohol + acceptor
-
671461
Sphingopyxis terrae
aldehyde + reduced acceptor
-
-
-
?
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.1
-
benzyl alcohol
mutant C472A
Sphingopyxis terrae
0.11
-
benzyl alcohol
wild type
Sphingopyxis terrae
0.44
-
1-Hexanol
mutant C472A; mutant H467A
Sphingopyxis terrae
0.45
-
1-Hexanol
mutant N511H; wild type
Sphingopyxis terrae
0.81
-
benzyl alkohol
mutant H467A
Sphingopyxis terrae
0.84
-
benzyl alcohol
mutant N511H
Sphingopyxis terrae
1
-
PEG 1000
mutant C382A
Sphingopyxis terrae
1.1
-
benzyl alcohol
mutant C297
Sphingopyxis terrae
1.1
-
PEG 1000
mutant C379A; mutant H467A; wild type
Sphingopyxis terrae
1.2
-
benzyl alcohol
mutant C382A
Sphingopyxis terrae
1.2
-
PEG 1000
mutant C472A; mutant N511H
Sphingopyxis terrae
1.4
-
benzyl alcohol
mutant C379A
Sphingopyxis terrae
1.7
-
1-Hexanol
mutant C379A; mutant C382A
Sphingopyxis terrae
2.3
-
PEG 6000
mutant C472A; mutant N511H
Sphingopyxis terrae
2.4
-
PEG 6000
mutant H467A; wild type
Sphingopyxis terrae
2.5
-
PEG 6000
mutant C297; mutant C379A; mutant C382A
Sphingopyxis terrae
6.7
-
TEG
wild type
Sphingopyxis terrae
6.8
-
TEG
mutant H467A
Sphingopyxis terrae
6.9
-
TEG
mutant C472A; mutant N511H
Sphingopyxis terrae
8.1
-
TEG
mutant C382A
Sphingopyxis terrae
8.3
-
TEG
mutant C379A
Sphingopyxis terrae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1-hexanol + acceptor
-
671461
Sphingopyxis terrae
n-hexanal + reduced acceptor
-
-
-
-
benzyl alcohol + acceptor
-
671461
Sphingopyxis terrae
benzaldehyde + reduced acceptor
-
-
-
-
primary alcohol + acceptor
-
671461
Sphingopyxis terrae
aldehyde + reduced acceptor
-
-
-
?
Other publictions for EC 1.1.99.20
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
725161
Liu
Catalytic mechanism of short e ...
Ensifer sp. AS08
Int. J. Mol. Sci.
14
1218-1231
2013
-
-
1
-
5
-
-
6
-
-
-
-
-
3
-
-
1
-
-
-
-
-
3
-
-
-
-
6
-
-
-
1
-
-
-
-
-
1
1
-
5
-
-
-
-
6
-
-
-
-
-
-
-
1
-
-
-
-
3
-
-
-
-
6
-
-
-
-
-
-
-
-
6
6
671546
Hu
Isolation of bacteria able to ...
Sphingopyxis macrogoltabida
Appl. Microbiol. Biotechnol.
73
1407-1413
2007
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
688864
Tani
Structure and conservation of ...
Sphingopyxis macrogoltabida 103, Sphingopyxis macrogoltabida, Sphingopyxis macrogoltabida 203, Sphingopyxis terrae
Microbiology
153
338-346
2007
-
2
2
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
671461
Ohta
Analysis of amino acid residue ...
Sphingopyxis terrae
Appl. Environ. Microbiol.
72
4388-4396
2006
-
-
-
-
-
-
-
21
-
-
-
-
-
5
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
21
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389911
Sugimoto
The first step in polyethylene ...
Sphingopyxis terrae
J. Bacteriol.
183
6694-6698
2001
-
-
1
-
-
-
-
10
1
-
2
-
-
7
-
-
1
-
-
-
-
-
12
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
10
1
-
2
-
-
-
-
1
-
-
-
-
12
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389910
Yasuda
-
Polyethylene glycol dehydrogen ...
Rhodoblastus acidophilus, Rhodoblastus acidophilus M402
FEMS Microbiol. Lett.
138
23-28
1996
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
1
-
18
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
18
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389907
Yamanaka
-
Inducible or constitutive poly ...
Flavobacterium sp., Pseudomonas sp.
J. Ferment. Bioeng.
67
300-302
1989
4
-
-
-
-
-
2
-
2
3
-
-
-
2
-
-
-
-
-
-
6
-
19
-
-
-
-
-
-
-
-
2
-
-
-
4
-
-
2
-
-
-
-
2
-
-
2
3
-
-
-
-
-
-
-
-
6
-
19
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389908
Yamanaka
-
Purification and characterizat ...
Flavobacterium sp.
J. Ferment. Bioeng.
67
324-330
1989
1
-
-
-
-
-
14
4
1
-
2
-
-
1
-
-
1
-
-
-
2
-
36
1
1
1
2
-
2
1
1
5
-
-
-
1
-
-
5
-
-
-
-
14
-
4
1
-
2
-
-
-
-
1
-
-
2
-
36
1
1
1
2
-
2
1
1
-
-
-
-
-
-
-
389909
Kawai
Biodegradation of polyethylene ...
Flavobacterium sp., Pseudomonas sp.
Arch. Microbiol.
146
125-129
1986
2
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
9
-
4
-
-
-
-
-
-
-
-
2
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
9
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389904
Kawai
Identification of the prosthet ...
Flavobacterium sp., Pseudomonas sp.
Agric. Biol. Chem.
49
1071-1076
1985
4
-
-
-
-
-
8
4
2
-
-
-
-
2
-
-
-
-
-
-
-
-
40
-
-
-
-
-
-
-
-
10
-
-
-
4
-
-
10
-
-
-
-
8
-
4
2
-
-
-
-
-
-
-
-
-
-
-
40
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389905
Kawai
-
Identification of reaction pro ...
Flavobacterium sp., Pseudomonas sp.
Agric. Biol. Chem.
47
1669-1671
1983
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389906
Kawai
Purification and characterizat ...
Flavobacterium sp., Pseudomonas sp.
Appl. Environ. Microbiol.
40
701-705
1980
-
-
-
-
-
-
22
4
-
4
4
-
-
3
-
-
2
-
-
-
2
-
28
2
2
2
4
-
2
2
6
2
-
-
-
-
-
-
2
-
-
-
-
22
-
4
-
4
4
-
-
-
-
2
-
-
2
-
28
2
2
2
4
-
2
2
6
-
-
-
-
-
-
-