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Literature summary for 1.1.99.24 extracted from
- Isolation and characterization of a hydroxyacid-oxoacid transhydrogenase from rat kidney mitochondria (1988), J. Biol. Chem., 263, 16872-16879.
General Stability
| General Stability | Organism |
|---|---|
| NAD+ stabilizes during ammonium sulfate precipitation | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not inhibited by anaerobiosis | Rattus norvegicus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0046 | - |
Succinic semialdehyde | - |
Rattus norvegicus |  |
| 0.018 | - |
2-oxoglutarate | - |
Rattus norvegicus | |
| 0.3 | - |
4-hydroxybutyrate | - |
Rattus norvegicus | |
| 0.42 | - |
D-2-hydroxyglutarate | - |
Rattus norvegicus | |
| 3 | - |
L-3-hydroxybutyrate | - |
Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Rattus norvegicus | 5739 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
Sprague-Dawley strain | - |
| Rattus norvegicus Sprague-Dawley | - |
Sprague-Dawley strain | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Rattus norvegicus | - |
| kidney | - |
Rattus norvegicus | - |
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0513 | - |
- |
Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-hydroxybutyrate + 2-oxoglutarate | hydrogen attached to hydroxyl-bearing C of 4-hydroxybutyrate is transferred to ketone-bearing C of 2-oxoglutarate, oxoacid not NAD+ or NADP+ acts as hydrogen acceptor | Rattus norvegicus | succinic semialdehyde + 2-hydroxyglutarate | - |
r | |
| 4-hydroxybutyrate + 2-oxoglutarate | hydrogen attached to hydroxyl-bearing C of 4-hydroxybutyrate is transferred to ketone-bearing C of 2-oxoglutarate, oxoacid not NAD+ or NADP+ acts as hydrogen acceptor | Rattus norvegicus Sprague-Dawley | succinic semialdehyde + 2-hydroxyglutarate | - |
r | |
| D-2-hydroxyglutarate + succinic semialdehyde | - |
Rattus norvegicus | 2-oxoglutarate + 4-hydroxybutyrate | - |
? | |
| D-2-hydroxyglutarate + succinic semialdehyde | - |
Rattus norvegicus Sprague-Dawley | 2-oxoglutarate + 4-hydroxybutyrate | - |
? | |
| L-3-hydroxybutyrate + 2-oxoglutarate | oxidized at 33% the rate of 4-hydroxybutyrate | Rattus norvegicus | acetoacetate + 2-oxoglutarate | - |
? | |
| L-3-hydroxybutyrate + 2-oxoglutarate | oxidized at 33% the rate of 4-hydroxybutyrate | Rattus norvegicus Sprague-Dawley | acetoacetate + 2-oxoglutarate | - |
? | |
| L-3-hydroxybutyrate + succinic semialdehyde | oxo-acids that can act as hydrogen acceptors are 2-oxoadipate, 24% as effective as 2-oxoglutarate and oxaloacetate, 19% as effective as 2-oxoglutarate, D-3-hydroxybutyrate, DL-2-hydroxybutyrate, D/L-malate, D/L-lactate, acetoacetate, 3-oxobutyrate, 3-oxoadipate do not act as hydrogen acceptors, poor acceptors are pyruvate and 2-oxobutyrate | Rattus norvegicus | acetoacetate + 4-hydroxybutyrate | - |
? | |
| L-3-hydroxybutyrate + succinic semialdehyde | 62.5% of activity with D-2-hydroxyglutarate | Rattus norvegicus | acetoacetate + 4-hydroxybutyrate | - |
? | |
| L-3-hydroxybutyrate + succinic semialdehyde | oxo-acids that can act as hydrogen acceptors are 2-oxoadipate, 24% as effective as 2-oxoglutarate and oxaloacetate, 19% as effective as 2-oxoglutarate, D-3-hydroxybutyrate, DL-2-hydroxybutyrate, D/L-malate, D/L-lactate, acetoacetate, 3-oxobutyrate, 3-oxoadipate do not act as hydrogen acceptors, poor acceptors are pyruvate and 2-oxobutyrate | Rattus norvegicus Sprague-Dawley | acetoacetate + 4-hydroxybutyrate | - |
? | |
| L-3-hydroxybutyrate + succinic semialdehyde | 62.5% of activity with D-2-hydroxyglutarate | Rattus norvegicus Sprague-Dawley | acetoacetate + 4-hydroxybutyrate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | reaction does not depend on NAD+ or NADP+ | Rattus norvegicus |
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