BRENDA - Enzyme Database
show all sequences of 1.1.99.24

Isolation and characterization of a hydroxyacid-oxoacid transhydrogenase from rat kidney mitochondria

Kaufman, E.E.; Nelson, T.; Fales, H.M.; Levin, D.M.; J. Biol. Chem. 263, 16872-16879 (1988)

Data extracted from this reference:

General Stability
General Stability
Organism
NAD+ stabilizes during ammonium sulfate precipitation
Rattus norvegicus
Inhibitors
Inhibitors
Commentary
Organism
Structure
additional information
not inhibited by anaerobiosis
Rattus norvegicus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0046
-
Succinic semialdehyde
-
Rattus norvegicus
0.018
-
2-oxoglutarate
-
Rattus norvegicus
0.3
-
4-hydroxybutyrate
-
Rattus norvegicus
0.42
-
D-2-hydroxyglutarate
-
Rattus norvegicus
3
-
L-3-hydroxybutyrate
-
Rattus norvegicus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrion
-
Rattus norvegicus
5739
-
Organism
Organism
UniProt
Commentary
Textmining
Rattus norvegicus
-
Sprague-Dawley strain
-
Rattus norvegicus Sprague-Dawley
-
Sprague-Dawley strain
-
Purification (Commentary)
Purification (Commentary)
Organism
partial
Rattus norvegicus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
brain
-
Rattus norvegicus
-
kidney
-
Rattus norvegicus
-
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0513
-
-
Rattus norvegicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
4-hydroxybutyrate + 2-oxoglutarate
hydrogen attached to hydroxyl-bearing C of 4-hydroxybutyrate is transferred to ketone-bearing C of 2-oxoglutarate, oxoacid not NAD+ or NADP+ acts as hydrogen acceptor
287797
Rattus norvegicus
succinic semialdehyde + 2-hydroxyglutarate
-
287797
Rattus norvegicus
r
4-hydroxybutyrate + 2-oxoglutarate
hydrogen attached to hydroxyl-bearing C of 4-hydroxybutyrate is transferred to ketone-bearing C of 2-oxoglutarate, oxoacid not NAD+ or NADP+ acts as hydrogen acceptor
287797
Rattus norvegicus Sprague-Dawley
succinic semialdehyde + 2-hydroxyglutarate
-
287797
Rattus norvegicus Sprague-Dawley
r
D-2-hydroxyglutarate + succinic semialdehyde
-
287797
Rattus norvegicus
2-oxoglutarate + 4-hydroxybutyrate
-
287797
Rattus norvegicus
?
D-2-hydroxyglutarate + succinic semialdehyde
-
287797
Rattus norvegicus Sprague-Dawley
2-oxoglutarate + 4-hydroxybutyrate
-
287797
Rattus norvegicus Sprague-Dawley
?
L-3-hydroxybutyrate + 2-oxoglutarate
oxidized at 33% the rate of 4-hydroxybutyrate
287797
Rattus norvegicus
acetoacetate + 2-oxoglutarate
-
287797
Rattus norvegicus
?
L-3-hydroxybutyrate + 2-oxoglutarate
oxidized at 33% the rate of 4-hydroxybutyrate
287797
Rattus norvegicus Sprague-Dawley
acetoacetate + 2-oxoglutarate
-
287797
Rattus norvegicus Sprague-Dawley
?
L-3-hydroxybutyrate + succinic semialdehyde
oxo-acids that can act as hydrogen acceptors are 2-oxoadipate, 24% as effective as 2-oxoglutarate and oxaloacetate, 19% as effective as 2-oxoglutarate, D-3-hydroxybutyrate, DL-2-hydroxybutyrate, D/L-malate, D/L-lactate, acetoacetate, 3-oxobutyrate, 3-oxoadipate do not act as hydrogen acceptors, poor acceptors are pyruvate and 2-oxobutyrate
287797
Rattus norvegicus
acetoacetate + 4-hydroxybutyrate
-
287797
Rattus norvegicus
?
L-3-hydroxybutyrate + succinic semialdehyde
62.5% of activity with D-2-hydroxyglutarate
287797
Rattus norvegicus
acetoacetate + 4-hydroxybutyrate
-
287797
Rattus norvegicus
?
L-3-hydroxybutyrate + succinic semialdehyde
oxo-acids that can act as hydrogen acceptors are 2-oxoadipate, 24% as effective as 2-oxoglutarate and oxaloacetate, 19% as effective as 2-oxoglutarate, D-3-hydroxybutyrate, DL-2-hydroxybutyrate, D/L-malate, D/L-lactate, acetoacetate, 3-oxobutyrate, 3-oxoadipate do not act as hydrogen acceptors, poor acceptors are pyruvate and 2-oxobutyrate
287797
Rattus norvegicus Sprague-Dawley
acetoacetate + 4-hydroxybutyrate
-
287797
Rattus norvegicus Sprague-Dawley
?
L-3-hydroxybutyrate + succinic semialdehyde
62.5% of activity with D-2-hydroxyglutarate
287797
Rattus norvegicus Sprague-Dawley
acetoacetate + 4-hydroxybutyrate
-
287797
Rattus norvegicus Sprague-Dawley
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Rattus norvegicus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Rattus norvegicus
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
reaction does not depend on NAD+ or NADP+
Rattus norvegicus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
reaction does not depend on NAD+ or NADP+
Rattus norvegicus
General Stability (protein specific)
General Stability
Organism
NAD+ stabilizes during ammonium sulfate precipitation
Rattus norvegicus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
additional information
not inhibited by anaerobiosis
Rattus norvegicus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0046
-
Succinic semialdehyde
-
Rattus norvegicus
0.018
-
2-oxoglutarate
-
Rattus norvegicus
0.3
-
4-hydroxybutyrate
-
Rattus norvegicus
0.42
-
D-2-hydroxyglutarate
-
Rattus norvegicus
3
-
L-3-hydroxybutyrate
-
Rattus norvegicus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrion
-
Rattus norvegicus
5739
-
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Rattus norvegicus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
brain
-
Rattus norvegicus
-
kidney
-
Rattus norvegicus
-
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0513
-
-
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
4-hydroxybutyrate + 2-oxoglutarate
hydrogen attached to hydroxyl-bearing C of 4-hydroxybutyrate is transferred to ketone-bearing C of 2-oxoglutarate, oxoacid not NAD+ or NADP+ acts as hydrogen acceptor
287797
Rattus norvegicus
succinic semialdehyde + 2-hydroxyglutarate
-
287797
Rattus norvegicus
r
4-hydroxybutyrate + 2-oxoglutarate
hydrogen attached to hydroxyl-bearing C of 4-hydroxybutyrate is transferred to ketone-bearing C of 2-oxoglutarate, oxoacid not NAD+ or NADP+ acts as hydrogen acceptor
287797
Rattus norvegicus Sprague-Dawley
succinic semialdehyde + 2-hydroxyglutarate
-
287797
Rattus norvegicus Sprague-Dawley
r
D-2-hydroxyglutarate + succinic semialdehyde
-
287797
Rattus norvegicus
2-oxoglutarate + 4-hydroxybutyrate
-
287797
Rattus norvegicus
?
D-2-hydroxyglutarate + succinic semialdehyde
-
287797
Rattus norvegicus Sprague-Dawley
2-oxoglutarate + 4-hydroxybutyrate
-
287797
Rattus norvegicus Sprague-Dawley
?
L-3-hydroxybutyrate + 2-oxoglutarate
oxidized at 33% the rate of 4-hydroxybutyrate
287797
Rattus norvegicus
acetoacetate + 2-oxoglutarate
-
287797
Rattus norvegicus
?
L-3-hydroxybutyrate + 2-oxoglutarate
oxidized at 33% the rate of 4-hydroxybutyrate
287797
Rattus norvegicus Sprague-Dawley
acetoacetate + 2-oxoglutarate
-
287797
Rattus norvegicus Sprague-Dawley
?
L-3-hydroxybutyrate + succinic semialdehyde
oxo-acids that can act as hydrogen acceptors are 2-oxoadipate, 24% as effective as 2-oxoglutarate and oxaloacetate, 19% as effective as 2-oxoglutarate, D-3-hydroxybutyrate, DL-2-hydroxybutyrate, D/L-malate, D/L-lactate, acetoacetate, 3-oxobutyrate, 3-oxoadipate do not act as hydrogen acceptors, poor acceptors are pyruvate and 2-oxobutyrate
287797
Rattus norvegicus
acetoacetate + 4-hydroxybutyrate
-
287797
Rattus norvegicus
?
L-3-hydroxybutyrate + succinic semialdehyde
62.5% of activity with D-2-hydroxyglutarate
287797
Rattus norvegicus
acetoacetate + 4-hydroxybutyrate
-
287797
Rattus norvegicus
?
L-3-hydroxybutyrate + succinic semialdehyde
oxo-acids that can act as hydrogen acceptors are 2-oxoadipate, 24% as effective as 2-oxoglutarate and oxaloacetate, 19% as effective as 2-oxoglutarate, D-3-hydroxybutyrate, DL-2-hydroxybutyrate, D/L-malate, D/L-lactate, acetoacetate, 3-oxobutyrate, 3-oxoadipate do not act as hydrogen acceptors, poor acceptors are pyruvate and 2-oxobutyrate
287797
Rattus norvegicus Sprague-Dawley
acetoacetate + 4-hydroxybutyrate
-
287797
Rattus norvegicus Sprague-Dawley
?
L-3-hydroxybutyrate + succinic semialdehyde
62.5% of activity with D-2-hydroxyglutarate
287797
Rattus norvegicus Sprague-Dawley
acetoacetate + 4-hydroxybutyrate
-
287797
Rattus norvegicus Sprague-Dawley
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Rattus norvegicus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Rattus norvegicus
Other publictions for EC 1.1.99.24
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741433
Yang
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1
1
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1
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-
-
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-
-
-
-
-
-
1
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1
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1
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1
1
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Diversity and evolutionary ana ...
Acanthamoeba castellanii, Acanthamoeba castellanii NEFF, Acytostelium subglobosum, Acytostelium subglobosum LB1, Aphanomyces astaci, Aphanomyces astaci APO3, Aphanomyces invadans, Aphanomyces invadans NJM9701, Capsaspora owczarzaki, Capsaspora owczarzaki ATCC 30864, Dictyostelium discoideum, Dictyostelium purpureum, Heterostelium pallidum, Phaeodactylum tricornutum, Phaeodactylum tricornutum CCAP 1055/1, Reticulomyxa filosa, Saprolegnia diclina, Saprolegnia diclina VS20, Saprolegnia parasitica, Saprolegnia parasitica CBS223.65, Thalassiosira oceanica, Thecamonas trahens, Thecamonas trahens ATCC 50062, Tieghemostelium lacteum
PLoS ONE
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e0166851
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14
-
34
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14
-
15
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
14
-
-
-
-
-
-
-
-
14
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
697277
Lyon
Enzymes involved in the metabo ...
Homo sapiens
Chem. Biol. Interact.
178
283-287
2009
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
1
-
2
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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1
-
-
1
-
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-
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-
-
-
664967
Kardon
Identification of the gene enc ...
Homo sapiens, Mus musculus, Rattus norvegicus
FEBS Lett.
580
2347-2350
2006
1
-
1
-
-
-
3
10
1
1
3
4
-
6
-
-
1
-
-
9
1
-
6
1
5
2
-
-
-
2
-
-
2
2
-
2
1
-
1
2
-
-
-
2
3
2
10
1
1
3
4
-
-
-
1
-
9
1
-
6
1
2
-
-
-
2
-
-
-
-
-
-
-
-
-
675230
Struys
Kinetic characterization of hu ...
Homo sapiens
J. Inherit. Metab. Dis.
28
921-930
2005
-
-
-
-
-
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-
-
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-
2
-
2
-
-
-
-
-
3
1
-
3
-
1
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
3
1
-
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
286147
Kaufman
An overview of gamma-hydroxybu ...
Rattus norvegicus
Neurochem. Res.
16
965-974
1991
-
-
-
-
-
-
-
5
-
-
-
-
-
1
-
-
-
-
-
-
1
-
4
-
-
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5
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1
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4
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-
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-
-
-
-
-
-
-
-
-
-
-
-
287797
Kaufman
Isolation and characterization ...
Rattus norvegicus, Rattus norvegicus Sprague-Dawley
J. Biol. Chem.
263
16872-16879
1988
-
-
-
-
-
1
1
5
2
-
-
-
-
164
-
-
1
-
-
4
1
-
10
-
-
1
-
-
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1
-
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1
-
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-
-
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-
1
-
-
1
-
1
-
5
2
-
-
-
-
-
-
1
-
4
1
-
10
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-