BRENDA - Enzyme Database
show all sequences of 1.1.99.24

Kinetic characterization of human hydroxyacid-oxoacid transhydrogenase: relevance to D-2-hydroxyglutaric and gamma-hydroxybutyric acidurias

Struys, E.A.; Verhoeven, N.M.; Ten Brink, H.J.; Wickenhagen, W.V.; Gibson, K.M.; Jakobs, C.; J. Inherit. Metab. Dis. 28, 921-930 (2005)

Data extracted from this reference:

Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3-hydroxybutyrate + 2-oxoglutarate
Homo sapiens
-
succinic semialdehyde + D-2-hydroxyglutarate
-
-
r
additional information
Homo sapiens
D-2-hydroxyglutaric aciduria is not associated to the enzyme activity
?
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Homo sapiens
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
fibroblast
of healthy subjects and patients with D-2-hydroxyglutaric aciduria
Homo sapiens
-
liver
-
Homo sapiens
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
assay method optimization, overview
Homo sapiens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3-hydroxybutyrate + 2-oxoglutarate
-
675230
Homo sapiens
succinic semialdehyde + D-2-hydroxyglutarate
-
-
-
r
3-hydroxybutyrate + 2-oxoglutarate
radiolabeled substrate 3-hydroxybutyrate, stereospecific reaction
675230
Homo sapiens
succinic semialdehyde + D-2-hydroxyglutarate
radiolabeled product D-2-hydroxyglutarate
-
-
r
additional information
D-2-hydroxyglutaric aciduria is not associated to the enzyme activity
675230
Homo sapiens
?
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
HOT
-
Homo sapiens
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Homo sapiens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Homo sapiens
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
no cofactor required
Homo sapiens
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
no cofactor required
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3-hydroxybutyrate + 2-oxoglutarate
Homo sapiens
-
succinic semialdehyde + D-2-hydroxyglutarate
-
-
r
additional information
Homo sapiens
D-2-hydroxyglutaric aciduria is not associated to the enzyme activity
?
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
fibroblast
of healthy subjects and patients with D-2-hydroxyglutaric aciduria
Homo sapiens
-
liver
-
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
assay method optimization, overview
Homo sapiens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3-hydroxybutyrate + 2-oxoglutarate
-
675230
Homo sapiens
succinic semialdehyde + D-2-hydroxyglutarate
-
-
-
r
3-hydroxybutyrate + 2-oxoglutarate
radiolabeled substrate 3-hydroxybutyrate, stereospecific reaction
675230
Homo sapiens
succinic semialdehyde + D-2-hydroxyglutarate
radiolabeled product D-2-hydroxyglutarate
-
-
r
additional information
D-2-hydroxyglutaric aciduria is not associated to the enzyme activity
675230
Homo sapiens
?
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Homo sapiens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Homo sapiens
Other publictions for EC 1.1.99.24
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741433
Yang
RNA interference-mediated knoc ...
Bemisia tabaci
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41201
2017
-
-
-
-
-
-
-
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-
-
-
-
3
-
-
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1
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1
1
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1
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1
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1
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1
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1
1
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741263
Gaona-Lopez
Diversity and evolutionary ana ...
Dictyostelium discoideum, Heterostelium pallidum, Capsaspora owczarzaki, Acytostelium subglobosum, Aphanomyces invadans, Saprolegnia parasitica, Thecamonas trahens, Phaeodactylum tricornutum, Dictyostelium purpureum, Tieghemostelium lacteum, Thalassiosira oceanica, Acanthamoeba castellanii, Saprolegnia diclina, Aphanomyces astaci, Reticulomyxa filosa, Acytostelium subglobosum LB1, Phaeodactylum tricornutum CCAP 1055/1, Aphanomyces astaci APO3, Saprolegnia parasitica CBS223.65, Aphanomyces invadans NJM9701, Capsaspora owczarzaki ATCC 30864, Saprolegnia diclina VS20, Thecamonas trahens ATCC 50062, Acanthamoeba castellanii NEFF
PLoS ONE
11
e0166851
2016
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-
-
-
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14
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49
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14
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15
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14
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-
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14
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
697277
Lyon
Enzymes involved in the metabo ...
Homo sapiens
Chem. Biol. Interact.
178
283-287
2009
-
-
-
-
-
-
-
-
-
-
-
-
-
2
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-
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-
-
1
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-
1
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2
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-
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-
-
-
1
-
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1
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1
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1
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664967
Kardon
Identification of the gene enc ...
Homo sapiens, Mus musculus, Rattus norvegicus
FEBS Lett.
580
2347-2350
2006
1
-
1
-
-
-
3
12
1
2
3
4
-
10
-
-
2
-
-
13
1
-
7
1
5
2
-
-
-
3
-
-
3
2
-
2
1
-
1
3
-
-
-
2
6
2
12
1
2
3
4
-
-
-
2
-
13
1
-
7
1
2
-
-
-
3
-
-
-
-
-
-
-
-
-
675230
Struys
Kinetic characterization of hu ...
Homo sapiens
J. Inherit. Metab. Dis.
28
921-930
2005
-
-
-
-
-
-
-
-
-
-
-
2
-
6
-
-
-
-
-
3
1
-
3
-
1
1
-
-
-
1
-
-
1
-
-
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-
-
-
1
-
-
-
-
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-
-
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-
2
-
-
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-
3
1
-
3
-
1
-
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-
1
-
-
-
-
-
-
-
-
-
286147
Kaufman
An overview of gamma-hydroxybu ...
Rattus norvegicus
Neurochem. Res.
16
965-974
1991
-
-
-
-
-
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5
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1
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2
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4
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5
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2
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4
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287797
Kaufman
Isolation and characterization ...
Rattus norvegicus, Rattus norvegicus Sprague-Dawley
J. Biol. Chem.
263
16872-16879
1988
-
-
-
-
-
1
1
5
2
-
-
-
-
165
-
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1
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-
4
1
-
10
-
-
1
-
-
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1
-
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1
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1
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1
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1
-
5
2
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1
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4
1
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10
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1
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1
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