Protein Variants | Comment | Organism |
---|---|---|
S116D | a single site mutation S116D alters the enzyme which in the wild type situation contains NAD(P)+ as nondissociable redox cofactor reacting in a ping-pong type mechanism to a dehydrogenase with dissociable NAD(P)+ as cosubstrate and a sequential reaction type | Zymomonas mobilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic parameters of the glucose dehydrogenase reaction catalyzed by mutant enzymes | Zymomonas mobilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zymomonas mobilis | Q07982 | - |
- |
Purification (Comment) | Organism |
---|---|
mutant enzyme S116D | Zymomonas mobilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
kinetic parameters of the glucose dehydrogenase reaction catalyzed by mutant enzymes | Zymomonas mobilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + D-fructose | - |
Zymomonas mobilis | D-glucono-1,5-lactone + D-sorbitol | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | a single site mutation S116D alters the enzyme which in the wild type situation contains NAD(P)+ as nondissociable redox cofactor reacting in a ping-pong type mechanism to a dehydrogenase with dissociable NAD(P)+ as cosubstrate and a sequential reaction type | Zymomonas mobilis | |
NADH | a single site mutation S116D alters the enzyme which in the wild type situation contains NAD(P)+ as nondissociable redox cofactor reacting in a ping-pong type mechanism to a dehydrogenase with dissociable NAD(P)+ as cosubstrate and a sequential reaction type | Zymomonas mobilis | |
NADP+ | a single site mutation S116D alters the enzyme which in the wild type situation contains NAD(P)+ as nondissociable redox cofactor reacting in a ping-pong type mechanism to a dehydrogenase with dissociable NAD(P)+ as cosubstrate and a sequential reaction type | Zymomonas mobilis | |
NADPH | a single site mutation S116D alters the enzyme which in the wild type situation contains NAD(P)+ as nondissociable redox cofactor reacting in a ping-pong type mechanism to a dehydrogenase with dissociable NAD(P)+ as cosubstrate and a sequential reaction type | Zymomonas mobilis |