Reference to 1.1.99.31; Id = 667575 - BRENDA Enzyme Database

0.11

-

(S)-Mandelate

pH 7.5, 4Â°C

Pseudomonas putida

0.12

-

(S)-Mandelate

pH 7.5, 20Â°C

Pseudomonas putida

-

Pseudomonas putida

(S)-mandelate + acceptor

acceptor: 2,6-dichloroindophenol plus phenazine methosulfate. The MDH reaction has two rate-limiting step of similar activation energies: the formation and breakdown of a distinct intermediate, with the latter step being slightly more rate limiting. MDH is capable of catalyzing the reverse reaction, the reoxidation of reduced MDH by the product ketoacid, benzoylformate. The transient intermediate is observed during the reverse reaction as well

667575

Pseudomonas putida

2-oxo-2-phenylacetate + reduced acceptor

-

r

134

-

(S)-Mandelate

pH 7.5, 4Â°C

Pseudomonas putida

360

-

(S)-Mandelate

pH 7.5, 20Â°C

Pseudomonas putida

FMN

-

Pseudomonas putida

FMN

-

Pseudomonas putida

0.11

-

(S)-Mandelate

pH 7.5, 4Â°C

Pseudomonas putida

0.12

-

(S)-Mandelate

pH 7.5, 20Â°C

Pseudomonas putida

-

Pseudomonas putida

(S)-mandelate + acceptor

acceptor: 2,6-dichloroindophenol plus phenazine methosulfate. The MDH reaction has two rate-limiting step of similar activation energies: the formation and breakdown of a distinct intermediate, with the latter step being slightly more rate limiting. MDH is capable of catalyzing the reverse reaction, the reoxidation of reduced MDH by the product ketoacid, benzoylformate. The transient intermediate is observed during the reverse reaction as well

667575

Pseudomonas putida

2-oxo-2-phenylacetate + reduced acceptor

-

r

134

-

(S)-Mandelate

pH 7.5, 4Â°C

Pseudomonas putida

360

-

(S)-Mandelate

pH 7.5, 20Â°C

Pseudomonas putida

740174

Chen

Stereoselective biotransformat ...

Escherichia coli

Bioresour. Bioproc.

4

2-2

2017

-

1

-

1

-

2

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

740119

Mazurenko

Immobilization of membrane-bou ...

Pseudomonas putida, Pseudomonas putida DSM 291

Bioelectrochemistry

104

65-70

2015

-

2

-

4

-

2

-

1

-

2

-

2

-

725744

Wang

-

A bi-enzymatic system for effi ...

Pseudomonas aeruginosa

J. Mol. Catal. B

94

47-50

2013

-

1

-

2

-

1

-

1

-

1

-

2

-

1

-

1

-

1

-

1

-

1

-

2

-

1

-

1

-

2

-

1

-

1

-

1

-

695329

Sukumar

Structures of the G81A mutant ...

Pseudomonas putida

Acta Crystallogr. Sect. D

65

543-552

2009

-

1

2

-

12

-

4

-

1

-

4

-

1

-

12

-

1

-

1

2

-

12

-

1

-

4

-

12

-

667577

Dewanti

Role of glycine 81 in (S)-mand ...

Pseudomonas putida

Biochemistry

43

10692-10700

2004

-

5

-

1

36

-

4

-

1

-

13

-

36

-

1

-

1

-

5

-

1

-

36

-

1

-

13

-

36

-

669259

Sukumar

High resolution structures of ...

Pseudomonas putida

J. Biol. Chem.

279

3749-3757

2004

-

1

-

3

-

1

-

2

-

1

-

1

-

2

-

678128

Dewanti

Esters of mandelic acid as sub ...

Pseudomonas putida

Biochemistry

43

1883-1890

2004

-

2

-

4

9

-

5

-

1

-

4

-

1

-

9

-

1

2

-

1

-

2

-

4

2

9

-

1

-

4

-

9

-

667575

Dewanti

A transient intermediate in th ...

Pseudomonas putida

Biochemistry

42

12893-12901

2003

-

2

-

5

-

1

-

1

-

2

-

1

-

1

-

2

-

1

-

1

-

2

-

667572

Xu

Arginine 165/arginine 277 pair ...

Pseudomonas putida

Biochemistry

41

12313-12319

2002

-

10

-

3

9

-

5

-

1

-

1

-

9

-

1

13

-

1

-

10

-

3

13

9

-

1

-

1

-

9

-

667570

Sukumar

Structure of an active soluble ...

Pseudomonas putida

Biochemistry

40

9870-9878

2001

-

1

-

3

-

1

-

667567

Lehoux

Role of arginine 277 in (S)-ma ...

Pseudomonas putida

Biochemistry

39

10055-10065

2000

-

4

-

7

-

4

-

1

-

2

-

7

-

4

-

7

-

1

-

2

-

7

-

667565

Xu

A highly active, soluble mutan ...

Pseudomonas putida

Biochemistry

38

12367-12376

1999

-

1

-

14

1

-

6

-

14

-

1

-

15

-

1

-

14

1

-

14

-

15

-

667566

Lehoux

(S)-Mandelate dehydrogenase fr ...

Pseudomonas putida

Biochemistry

38

9948-9955

1999

-

3

-

1

-

5

-

1

-

2

-

2

-

3

-

1

-

1

-

2

-

2

-

678122

Lehoux

(S)-Mandelate dehydrogenase fr ...

Pseudomonas putida

Biochemistry

38

5836-5848

1999

-

1

-

5

11

-

4

-

1

-

14

-

1

-

1

-

1

-

5

1

11

-

1

-

14

-

667458

Illias

L-Mandelate dehydrogenase from ...

Rhodotorula graminis

Biochem. J.

333

107-115

1998

-

1

-

6

2

-

8

-

1

-

3

-

1

-

8

-

1

-

6

2

-

1

-

3

-

8

-

667457

Smekal

Substrate analogues as probes ...

Rhodotorula graminis

Biochem. J.

297

647-652

1994

-

11

-

3

-

11

-

12

-

11

-

11

-

12

-

667455

Smekal

L-mandelate dehydrogenase from ...

Rhodotorula graminis

Biochem. J.

290

103-107

1993

-

4

3

-

7

-

3

-

2

-

3

-

1

4

-

1

-

4

3

-

3

-

3

-

667564

Mitra

A novel structural basis for m ...

Pseudomonas putida

Biochemistry

32

12959-12967

1993

-

1

-

1

-

6

2

-

6

-

1

-

1

2

-

1

-

4

-

1

-

1

-

1

-

6

2

-

1

-

1

2

-

4

-

669678

Fewson

Comparison of mandelate dehydr ...

Acinetobacter calcoaceticus

J. Gen. Microbiol.

134

967-974

1988

-

1

-

4

-

1

-

2

-

1

-

1

-

1

-

1

-

A transient intermediate in the reaction catalyzed by (S)-mandelate dehydrogenase from Pseudomonas putida

Data extracted from this reference: