Data extracted from this reference:
KM Value [mM]
0.11
(S)-Mandelate
pH 7.5, 4°C
Pseudomonas putida
0.12
(S)-Mandelate
pH 7.5, 20°C
Pseudomonas putida
Organism
Purification (Commentary)
Substrates and Products (Substrate)
(S)-mandelate + acceptor
acceptor: 2,6-dichloroindophenol plus phenazine methosulfate. The MDH reaction has two rate-limiting step of similar activation energies: the formation and breakdown of a distinct intermediate, with the latter step being slightly more rate limiting. MDH is capable of catalyzing the reverse reaction, the reoxidation of reduced MDH by the product ketoacid, benzoylformate. The transient intermediate is observed during the reverse reaction as well
667575
Pseudomonas putida
2-oxo-2-phenylacetate + reduced acceptor
r
Turnover Number [1/s]
134
(S)-Mandelate
pH 7.5, 4°C
Pseudomonas putida
360
(S)-Mandelate
pH 7.5, 20°C
Pseudomonas putida
Cofactor
Cofactor (protein specific)
KM Value [mM] (protein specific)
0.11
(S)-Mandelate
pH 7.5, 4°C
Pseudomonas putida
0.12
(S)-Mandelate
pH 7.5, 20°C
Pseudomonas putida
Purification (Commentary) (protein specific)
Substrates and Products (Substrate) (protein specific)
(S)-mandelate + acceptor
acceptor: 2,6-dichloroindophenol plus phenazine methosulfate. The MDH reaction has two rate-limiting step of similar activation energies: the formation and breakdown of a distinct intermediate, with the latter step being slightly more rate limiting. MDH is capable of catalyzing the reverse reaction, the reoxidation of reduced MDH by the product ketoacid, benzoylformate. The transient intermediate is observed during the reverse reaction as well
667575
Pseudomonas putida
2-oxo-2-phenylacetate + reduced acceptor
r
Turnover Number [1/s] (protein specific)
134
(S)-Mandelate
pH 7.5, 4°C
Pseudomonas putida
360
(S)-Mandelate
pH 7.5, 20°C
Pseudomonas putida
Other publictions for EC 1.1.99.31
740174
Chen
Stereoselective biotransformat ...
Escherichia coli
Bioresour. Bioproc.
4
2-2
2017
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1
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1
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1
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1
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-
740119
Mazurenko
Immobilization of membrane-bou ...
Pseudomonas putida, Pseudomonas putida DSM 291
Bioelectrochemistry
104
65-70
2015
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2
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4
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725744
Wang
-
A bi-enzymatic system for effi ...
Pseudomonas aeruginosa
J. Mol. Catal. B
94
47-50
2013
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1
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1
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1
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2
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1
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1
1
1
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1
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-
695329
Sukumar
Structures of the G81A mutant ...
Pseudomonas putida
Acta Crystallogr. Sect. D
65
543-552
2009
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-
1
1
2
-
-
12
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4
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1
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4
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1
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12
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1
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1
1
1
2
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12
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1
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-
4
-
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-
12
-
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-
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-
667577
Dewanti
Role of glycine 81 in (S)-mand ...
Pseudomonas putida
Biochemistry
43
10692-10700
2004
-
-
-
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5
-
1
36
-
-
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4
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1
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13
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36
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1
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1
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5
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1
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36
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1
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13
-
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36
-
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669259
Sukumar
High resolution structures of ...
Pseudomonas putida
J. Biol. Chem.
279
3749-3757
2004
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1
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3
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1
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1
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2
-
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-
678128
Dewanti
Esters of mandelic acid as sub ...
Pseudomonas putida
Biochemistry
43
1883-1890
2004
-
-
-
-
2
-
4
9
-
-
-
-
-
5
-
-
1
-
-
-
-
-
4
-
1
-
-
-
9
-
-
-
1
2
-
-
-
-
-
1
-
2
-
-
4
2
9
-
-
-
-
-
-
-
1
-
-
-
-
4
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
667575
Dewanti
A transient intermediate in th ...
Pseudomonas putida
Biochemistry
42
12893-12901
2003
-
-
-
-
-
-
-
2
-
-
-
-
-
5
-
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1
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1
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2
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1
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1
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2
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1
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1
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-
2
-
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-
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-
667572
Xu
Arginine 165/arginine 277 pair ...
Pseudomonas putida
Biochemistry
41
12313-12319
2002
-
-
-
-
10
-
3
9
-
-
-
-
-
5
-
-
1
-
-
-
-
1
1
-
-
-
-
-
9
-
-
-
1
13
-
-
-
-
-
1
-
10
-
-
3
13
9
-
-
-
-
-
-
-
1
-
-
-
1
1
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
667570
Sukumar
Structure of an active soluble ...
Pseudomonas putida
Biochemistry
40
9870-9878
2001
-
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1
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3
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1
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-
667567
Lehoux
Role of arginine 277 in (S)-ma ...
Pseudomonas putida
Biochemistry
39
10055-10065
2000
-
-
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4
-
-
7
-
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-
4
-
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1
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2
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7
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4
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7
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1
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2
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7
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-
-
-
-
667565
Xu
A highly active, soluble mutan ...
Pseudomonas putida
Biochemistry
38
12367-12376
1999
-
-
-
-
1
-
-
14
1
-
-
-
-
6
-
-
-
-
-
-
-
-
14
-
1
-
-
-
15
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
14
1
-
-
-
-
-
-
-
-
-
-
-
14
-
-
-
-
15
-
-
-
-
-
-
-
-
-
-
667566
Lehoux
(S)-Mandelate dehydrogenase fr ...
Pseudomonas putida
Biochemistry
38
9948-9955
1999
-
-
-
-
3
-
-
1
-
-
-
-
-
5
-
-
1
-
-
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-
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2
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-
2
-
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3
-
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1
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-
1
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-
2
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
678122
Lehoux
(S)-Mandelate dehydrogenase fr ...
Pseudomonas putida
Biochemistry
38
5836-5848
1999
-
-
1
-
-
-
5
11
-
-
-
-
-
4
-
-
1
-
-
-
-
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14
-
1
-
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-
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1
1
-
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-
1
1
-
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5
1
11
-
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1
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14
-
-
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-
667458
Illias
L-Mandelate dehydrogenase from ...
Rhodotorula graminis
Biochem. J.
333
107-115
1998
-
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1
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6
2
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8
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1
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3
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1
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8
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1
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6
2
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1
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3
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8
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667457
Smekal
Substrate analogues as probes ...
Rhodotorula graminis
Biochem. J.
297
647-652
1994
-
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11
-
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3
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11
-
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12
-
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-
-
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-
11
-
-
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-
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-
-
-
-
11
-
-
-
-
12
-
-
-
-
-
-
-
-
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-
667455
Smekal
L-mandelate dehydrogenase from ...
Rhodotorula graminis
Biochem. J.
290
103-107
1993
-
-
-
-
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4
3
-
-
-
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-
7
-
-
-
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-
-
-
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3
-
2
-
-
-
3
-
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1
4
-
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1
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4
4
3
-
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3
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
667564
Mitra
A novel structural basis for m ...
Pseudomonas putida
Biochemistry
32
12959-12967
1993
-
-
1
-
1
1
-
6
2
-
-
-
-
6
-
-
1
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-
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-
1
2
-
1
-
-
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4
-
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1
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1
1
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1
1
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6
2
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1
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1
2
-
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-
4
-
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-
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-
669678
Fewson
Comparison of mandelate dehydr ...
Acinetobacter calcoaceticus
J. Gen. Microbiol.
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1988
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