BRENDA - Enzyme Database
show all sequences of 1.1.99.35

Increasing the thermal stability of the water-soluble pyrroloquinoline quinone glucose dehydrogenase by single amino acid replacement

Sode, K.; Ootera, T.; Shirahane, M.; Witarto, A.B.; Igarashi, S.; Yoshida, H.; Enzyme Microb. Technol. 26, 491-496 (2000)

Data extracted from this reference:

Engineering
Protein Variants
Commentary
Organism
S231C
substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 3%, D-mannose 8%, D-allose 46%, 3-O-methyl-D-glucose 76%, D-galactose 14%, D-xylose 8%, D-lactose 69%, D-maltose 69%, respectively
Acinetobacter calcoaceticus
S231D
substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 2%, D-mannose 9%, D-allose 38%, 3-O-methyl-D-glucose 71%, D-galactose 12%, D-xylose 8%, D-lactose 54%, D-maltose 38%, respectively
Acinetobacter calcoaceticus
S231H
substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 2%, D-mannose 12%, D-allose 57%, 3-O-methyl-D-glucose 86%, D-galactose 17%, D-xylose 7%, D-lactose 56%, D-maltose 38%, respectively
Acinetobacter calcoaceticus
S231K
increase in thermal stability. Substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 5%, D-mannose 10%, D-allose 43%, 3-O-methyl-D-glucose 82%, D-galactose 15%, D-xylose 5%, D-lactose 59%, D-maltose 70%, respectively
Acinetobacter calcoaceticus
S231L
substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 6%, D-mannose 13%, D-allose 62%, 3-O-methyl-D-glucose 105%, D-galactose 20%, D-xylose 12%, D-lactose 73%, D-maltose 76%, respectively
Acinetobacter calcoaceticus
S231M
substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 5%, D-mannose 9%, D-allose 43%, 3-O-methyl-D-glucose 80%, D-galactose 10%, D-xylose 8%, D-lactose 56%, D-maltose 41%, respectively
Acinetobacter calcoaceticus
S231N
substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 5%, D-mannose 12%, D-allose 61%, 3-O-methyl-D-glucose 109%, D-galactose 18%, D-xylose 15%, D-lactose 66%, D-maltose 51%, respectively
Acinetobacter calcoaceticus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
25
-
D-glucose
wild-type, pH 7.0
Acinetobacter calcoaceticus
27
-
D-glucose
mutant S231K, pH 7.0
Acinetobacter calcoaceticus
Organism
Organism
UniProt
Commentary
Textmining
Acinetobacter calcoaceticus
-
-
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2977
-
wild-type, pH 7.0, at 50 mM D-glucose
Acinetobacter calcoaceticus
3313
-
mutant S231K, pH 7.0, at 100 mM D-glucose
Acinetobacter calcoaceticus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2-deoxy-D-glucose + oxidized 2,6-dichlorophenolindophenol
4% of the activity with D-glucose
639218
Acinetobacter calcoaceticus
2-deoxy-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
3-O-methyl-D-glucose + oxidized 2,6-dichlorophenolindophenol
81% of the activity with D-glucose
639218
Acinetobacter calcoaceticus
3-O-methyl-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-allose + oxidized 2,6-dichlorophenolindophenol
47% of the activity with D-glucose
639218
Acinetobacter calcoaceticus
D-allono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-galactose + oxidized 2,6-dichlorophenolindophenol
11% of the activity with D-glucose
639218
Acinetobacter calcoaceticus
D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-glucose + oxidized 2,6-dichlorophenolindophenol
-
639218
Acinetobacter calcoaceticus
D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-mannose + oxidized 2,6-dichlorophenolindophenol
13% of the activity with D-glucose
639218
Acinetobacter calcoaceticus
D-mannono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-xylose + oxidized 2,6-dichlorophenolindophenol
7% of the activity with D-glucose
639218
Acinetobacter calcoaceticus
D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
lactose + oxidized 2,6-dichlorophenolindophenol
61% of the activity with D-glucose
639218
Acinetobacter calcoaceticus
lactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
maltose + oxidized 2,6-dichlorophenolindophenol
61% of the activity with D-glucose
639218
Acinetobacter calcoaceticus
maltono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
55
-
wild-type, 5 min, 50% loss of activity. Mutant S231K, 50% loss of activity after 41 min
Acinetobacter calcoaceticus
Engineering (protein specific)
Protein Variants
Commentary
Organism
S231C
substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 3%, D-mannose 8%, D-allose 46%, 3-O-methyl-D-glucose 76%, D-galactose 14%, D-xylose 8%, D-lactose 69%, D-maltose 69%, respectively
Acinetobacter calcoaceticus
S231D
substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 2%, D-mannose 9%, D-allose 38%, 3-O-methyl-D-glucose 71%, D-galactose 12%, D-xylose 8%, D-lactose 54%, D-maltose 38%, respectively
Acinetobacter calcoaceticus
S231H
substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 2%, D-mannose 12%, D-allose 57%, 3-O-methyl-D-glucose 86%, D-galactose 17%, D-xylose 7%, D-lactose 56%, D-maltose 38%, respectively
Acinetobacter calcoaceticus
S231K
increase in thermal stability. Substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 5%, D-mannose 10%, D-allose 43%, 3-O-methyl-D-glucose 82%, D-galactose 15%, D-xylose 5%, D-lactose 59%, D-maltose 70%, respectively
Acinetobacter calcoaceticus
S231L
substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 6%, D-mannose 13%, D-allose 62%, 3-O-methyl-D-glucose 105%, D-galactose 20%, D-xylose 12%, D-lactose 73%, D-maltose 76%, respectively
Acinetobacter calcoaceticus
S231M
substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 5%, D-mannose 9%, D-allose 43%, 3-O-methyl-D-glucose 80%, D-galactose 10%, D-xylose 8%, D-lactose 56%, D-maltose 41%, respectively
Acinetobacter calcoaceticus
S231N
substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 5%, D-mannose 12%, D-allose 61%, 3-O-methyl-D-glucose 109%, D-galactose 18%, D-xylose 15%, D-lactose 66%, D-maltose 51%, respectively
Acinetobacter calcoaceticus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
25
-
D-glucose
wild-type, pH 7.0
Acinetobacter calcoaceticus
27
-
D-glucose
mutant S231K, pH 7.0
Acinetobacter calcoaceticus
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2977
-
wild-type, pH 7.0, at 50 mM D-glucose
Acinetobacter calcoaceticus
3313
-
mutant S231K, pH 7.0, at 100 mM D-glucose
Acinetobacter calcoaceticus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2-deoxy-D-glucose + oxidized 2,6-dichlorophenolindophenol
4% of the activity with D-glucose
639218
Acinetobacter calcoaceticus
2-deoxy-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
3-O-methyl-D-glucose + oxidized 2,6-dichlorophenolindophenol
81% of the activity with D-glucose
639218
Acinetobacter calcoaceticus
3-O-methyl-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-allose + oxidized 2,6-dichlorophenolindophenol
47% of the activity with D-glucose
639218
Acinetobacter calcoaceticus
D-allono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-galactose + oxidized 2,6-dichlorophenolindophenol
11% of the activity with D-glucose
639218
Acinetobacter calcoaceticus
D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-glucose + oxidized 2,6-dichlorophenolindophenol
-
639218
Acinetobacter calcoaceticus
D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-mannose + oxidized 2,6-dichlorophenolindophenol
13% of the activity with D-glucose
639218
Acinetobacter calcoaceticus
D-mannono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-xylose + oxidized 2,6-dichlorophenolindophenol
7% of the activity with D-glucose
639218
Acinetobacter calcoaceticus
D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
lactose + oxidized 2,6-dichlorophenolindophenol
61% of the activity with D-glucose
639218
Acinetobacter calcoaceticus
lactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
maltose + oxidized 2,6-dichlorophenolindophenol
61% of the activity with D-glucose
639218
Acinetobacter calcoaceticus
maltono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
55
-
wild-type, 5 min, 50% loss of activity. Mutant S231K, 50% loss of activity after 41 min
Acinetobacter calcoaceticus
Other publictions for EC 1.1.99.35
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739914
An
Regulation of pyrroloquinoline ...
Pseudomonas putida, Pseudomonas putida KT 2240
Appl. Environ. Microbiol.
82
4955-4964
2016
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42
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1
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4
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1
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1
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739848
Flexer
Wired pyrroloquinoline quinone ...
Acinetobacter calcoaceticus
Anal. Chem.
86
2465-2473
2014
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2
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1
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739872
Ben Farhat
Coexpression of the pyrroloqui ...
Serratia marcescens, Serratia marcescens CTM 50650
Appl. Biochem. Biotechnol.
170
1738-1750
2013
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4
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739873
Stredansky
Monitoring of PQQ-dependent gl ...
Acinetobacter calcoaceticus
Appl. Biochem. Biotechnol.
171
1032-1041
2013
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1
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13
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3
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1
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1
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13
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12
12
724836
Schubart
-
A pyrroloquinolinequinone-depe ...
Acinetobacter calcoaceticus
Electrochim. Acta
82
224-232
2012
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1
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1
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1
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1
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724852
Yu
-
High-level production of solub ...
Acinetobacter calcoaceticus, Acinetobacter calcoaceticus L.M.D. 79.41
Eng. Life Sci.
12
574-582
2012
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1
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1
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1
10
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3
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1
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1
2
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10
1
2
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1
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1
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1
1
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1
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1
10
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1
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2
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10
1
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1
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723943
Flexer
Efficient direct electron tran ...
Acinetobacter calcoaceticus
Anal. Chem.
83
5721-5727
2011
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1
1
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1
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1
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5
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2
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3
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1
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1
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1
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2
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724172
Durand
Designing a highly active solu ...
Acinetobacter calcoaceticus
Biochem. Biophys. Res. Commun.
402
750-754
2010
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1
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1
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2
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1
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1
1
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655127
Tanaka
Increasing stability of water- ...
Acinetobacter calcoaceticus
BMC Biochem.
6
1
2005
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3
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4
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1
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5
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5
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655929
Igarashi
Construction and characterizat ...
Acinetobacter calcoaceticus
J. Biochem. Biophys. Methods
61
331-338
2004
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1
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1
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12
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1
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6
1
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12
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6
1
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654863
Oubrie
Structure and mechanism of sol ...
Acinetobacter calcoaceticus
Biochim. Biophys. Acta
1647
143-151
2003
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1
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1
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654398
Sode
-
Construction of engineered wat ...
Acinetobacter calcoaceticus
Biocatal. Biotransform.
20
405-412
2002
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5
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18
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1
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6
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2
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18
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5
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18
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6
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18
-
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18
18
655363
Yoshida
-
Secretion of water soluble pyr ...
Acinetobacter calcoaceticus
Enzyme Microb. Technol.
30
312-318
2002
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1
1
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1
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4
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1
1
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1
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1
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2
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639218
Sode
Increasing the thermal stabili ...
Acinetobacter calcoaceticus
Enzyme Microb. Technol.
26
491-496
2000
-
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7
-
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2
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1
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2
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9
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1
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7
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2
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2
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9
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1
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702202
Dewanti
Ca2+-assisted, direct hydride ...
Acinetobacter calcoaceticus
Biochemistry
39
9384-9392
2000
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4
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1
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702811
Kojima
-
The production of soluble pyrr ...
Acinetobacter calcoaceticus
Biotechnol. Lett.
22
1343-1347
2000
-
1
1
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1
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1
1
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639204
Oubrie
The 1.7 A crystal structure of ...
Acinetobacter calcoaceticus
J. Mol. Biol.
289
319-333
1999
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1
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1
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4
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1
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1
1
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1
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639216
Igarashi
Construction and characterizat ...
Acinetobacter calcoaceticus
Biochem. Biophys. Res. Commun.
264
820-824
1999
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12
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18
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1
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9
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18
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12
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18
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9
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18
-
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18
18
639220
Oubrie
Active-site structure of the s ...
Acinetobacter calcoaceticus
Proc. Natl. Acad. Sci. USA
96
11787-11791
1999
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1
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1
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3
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1
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1
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1
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639209
Olsthoorn
On the mechanism and specifici ...
Acinetobacter calcoaceticus
Biochemistry
37
13854-13861
1998
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2
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16
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1
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1
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16
-
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1
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17
17
703532
Olsthoorn
Negative cooperativity in the ...
Acinetobacter calcoaceticus
Eur. J. Biochem.
255
255-261
1998
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-
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2
1
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2
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6
-
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1
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4
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3
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2
3
1
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2
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4
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703530
Olsthoorn
Ca2+ and its substitutes have ...
Acinetobacter calcoaceticus
Eur. J. Biochem.
247
659-665
1997
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-
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1
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1
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702726
Matsushita
-
Soluble and membrane-bound qui ...
Acinetobacter calcoaceticus
Biosci. Biotechnol. Biochem.
59
1548-1555
1995
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5
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1
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1
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1
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1
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5
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705107
Schlunegger
Crystallization and preliminar ...
Acinetobacter calcoaceticus
J. Mol. Biol.
233
784-786
1993
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1
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