BRENDA - Enzyme Database show
show all sequences of 1.1.99.35

Construction of engineered water-soluble PQQ glucose dehydrogenase with improved substrate specificity

Sode, K.; Igarashi, S.; Morimoto, A.; Yoshida, H.; Biocatal. Biotransform. 20, 405-412 (2002)
No PubMed abstract available

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
D448N
substrate specificity: D-glucose 100% activity, 3-O-methyl-D-glucose 72%, D-allose 39%, D-galactose 14%, D-maltose 36%, D-lactose 48%, respectively
Acinetobacter calcoaceticus
D456N
substrate specificity: D-glucose 100% activity, 3-O-methyl-D-glucose 78%, D-allose 43%, D-galactose 16%, D-maltose 41%, D-lactose 59%, respectively
Acinetobacter calcoaceticus
DD457N
substrate specificity: D-glucose 100% activity, 3-O-methyl-D-glucose 80%, D-allose 46%, D-galactose 11%, D-maltose 32%, D-lactose 43%, respectively
Acinetobacter calcoaceticus
N452T
substrate specificity: D-glucose 100% activity, 3-O-methyl-D-glucose 59%, D-allose 33%, D-galactose 5%, D-maltose 30%, D-lactose 31%, respectively
Acinetobacter calcoaceticus
N462H
substrate specificity: D-glucose 100% activity, 3-O-methyl-D-glucose 53%, D-allose 32%, D-galactose 2%, D-maltose 25%, D-lactose 31%, respectively
Acinetobacter calcoaceticus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7
-
D-galactose
mutant N462H, pH 7.0
Acinetobacter calcoaceticus
3.7
-
D-galactose
mutant N452T, pH 7.0
Acinetobacter calcoaceticus
5.3
-
D-galactose
wild-type, pH 7.0
Acinetobacter calcoaceticus
12.3
-
D-glucose
mutant N462H, pH 7.0
Acinetobacter calcoaceticus
12.5
-
D-glucose
mutant N452T, pH 7.0
Acinetobacter calcoaceticus
16
-
D-maltose
mutant N462H, pH 7.0
Acinetobacter calcoaceticus
18
-
D-lactose
mutant N462H, pH 7.0
Acinetobacter calcoaceticus
18.9
-
D-lactose
wild-type, pH 7.0
Acinetobacter calcoaceticus
25
-
D-glucose
wild-type, pH 7.0
Acinetobacter calcoaceticus
26
-
D-maltose
wild-type, pH 7.0
Acinetobacter calcoaceticus
27.6
-
3-O-methyl-D-glucose
mutant N452T, pH 7.0
Acinetobacter calcoaceticus
28.7
-
3-O-methyl-D-glucose
wild-type, pH 7.0
Acinetobacter calcoaceticus
28.8
-
3-O-methyl-D-glucose
mutant N462H, pH 7.0
Acinetobacter calcoaceticus
32.5
-
D-Allose
mutant N462H, pH 7.0
Acinetobacter calcoaceticus
33.6
-
D-lactose
mutant N452T, pH 7.0
Acinetobacter calcoaceticus
35.5
-
D-Allose
wild-type, pH 7.0
Acinetobacter calcoaceticus
38.7
-
D-Allose
mutant N452T, pH 7.0
Acinetobacter calcoaceticus
46.5
-
D-maltose
mutant N452T, pH 7.0
Acinetobacter calcoaceticus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Acinetobacter calcoaceticus
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-O-methyl-D-glucose + oxidized 2,6-dichlorophenolindophenol
81% of the activity with D-glucose
654398
Acinetobacter calcoaceticus
3-O-methyl-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-allose + oxidized 2,6-dichlorophenolindophenol
47% of the activity with D-glucose
654398
Acinetobacter calcoaceticus
D-allono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-galactose + oxidized 2,6-dichlorophenolindophenol
11% of the activity with D-glucose
654398
Acinetobacter calcoaceticus
D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-glucose + oxidized 2,6-dichlorophenolindophenol
-
654398
Acinetobacter calcoaceticus
D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
lactose + oxidized 2,6-dichlorophenolindophenol
61% of the activity with D-glucose
654398
Acinetobacter calcoaceticus
lactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
maltose + oxidized 2,6-dichlorophenolindophenol
61% of the activity with D-glucose
654398
Acinetobacter calcoaceticus
maltono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
69
-
D-galactose
mutant N462H, pH 7.0
Acinetobacter calcoaceticus
72
-
D-galactose
mutant N452T, pH 7.0
Acinetobacter calcoaceticus
232
-
D-galactose
wild-type, pH 7.0
Acinetobacter calcoaceticus
574
-
D-lactose
mutant N462H, pH 7.0
Acinetobacter calcoaceticus
588
-
D-maltose
mutant N462H, pH 7.0
Acinetobacter calcoaceticus
949
-
D-Allose
mutant N452T, pH 7.0
Acinetobacter calcoaceticus
1002
-
D-maltose
mutant N452T, pH 7.0
Acinetobacter calcoaceticus
1035
-
D-Allose
mutant N462H, pH 7.0
Acinetobacter calcoaceticus
1038
-
D-lactose
mutant N452T, pH 7.0
Acinetobacter calcoaceticus
1064
-
3-O-methyl-D-glucose
mutant N462H, pH 7.0
Acinetobacter calcoaceticus
1253
-
3-O-methyl-D-glucose
mutant N452T, pH 7.0
Acinetobacter calcoaceticus
1399
-
D-glucose
mutant N462H, pH 7.0
Acinetobacter calcoaceticus
1659
-
D-lactose
wild-type, pH 7.0
Acinetobacter calcoaceticus
1791
-
D-glucose
mutant N452T, pH 7.0
Acinetobacter calcoaceticus
1930
-
D-maltose
wild-type, pH 7.0
Acinetobacter calcoaceticus
2509
-
D-Allose
wild-type, pH 7.0
Acinetobacter calcoaceticus
3011
-
3-O-methyl-D-glucose
wild-type, pH 7.0
Acinetobacter calcoaceticus
3860
-
D-glucose
wild-type, pH 7.0
Acinetobacter calcoaceticus
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
D448N
substrate specificity: D-glucose 100% activity, 3-O-methyl-D-glucose 72%, D-allose 39%, D-galactose 14%, D-maltose 36%, D-lactose 48%, respectively
Acinetobacter calcoaceticus
D456N
substrate specificity: D-glucose 100% activity, 3-O-methyl-D-glucose 78%, D-allose 43%, D-galactose 16%, D-maltose 41%, D-lactose 59%, respectively
Acinetobacter calcoaceticus
DD457N
substrate specificity: D-glucose 100% activity, 3-O-methyl-D-glucose 80%, D-allose 46%, D-galactose 11%, D-maltose 32%, D-lactose 43%, respectively
Acinetobacter calcoaceticus
N452T
substrate specificity: D-glucose 100% activity, 3-O-methyl-D-glucose 59%, D-allose 33%, D-galactose 5%, D-maltose 30%, D-lactose 31%, respectively
Acinetobacter calcoaceticus
N462H
substrate specificity: D-glucose 100% activity, 3-O-methyl-D-glucose 53%, D-allose 32%, D-galactose 2%, D-maltose 25%, D-lactose 31%, respectively
Acinetobacter calcoaceticus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7
-
D-galactose
mutant N462H, pH 7.0
Acinetobacter calcoaceticus
3.7
-
D-galactose
mutant N452T, pH 7.0
Acinetobacter calcoaceticus
5.3
-
D-galactose
wild-type, pH 7.0
Acinetobacter calcoaceticus
12.3
-
D-glucose
mutant N462H, pH 7.0
Acinetobacter calcoaceticus
12.5
-
D-glucose
mutant N452T, pH 7.0
Acinetobacter calcoaceticus
16
-
D-maltose
mutant N462H, pH 7.0
Acinetobacter calcoaceticus
18
-
D-lactose
mutant N462H, pH 7.0
Acinetobacter calcoaceticus
18.9
-
D-lactose
wild-type, pH 7.0
Acinetobacter calcoaceticus
25
-
D-glucose
wild-type, pH 7.0
Acinetobacter calcoaceticus
26
-
D-maltose
wild-type, pH 7.0
Acinetobacter calcoaceticus
27.6
-
3-O-methyl-D-glucose
mutant N452T, pH 7.0
Acinetobacter calcoaceticus
28.7
-
3-O-methyl-D-glucose
wild-type, pH 7.0
Acinetobacter calcoaceticus
28.8
-
3-O-methyl-D-glucose
mutant N462H, pH 7.0
Acinetobacter calcoaceticus
32.5
-
D-Allose
mutant N462H, pH 7.0
Acinetobacter calcoaceticus
33.6
-
D-lactose
mutant N452T, pH 7.0
Acinetobacter calcoaceticus
35.5
-
D-Allose
wild-type, pH 7.0
Acinetobacter calcoaceticus
38.7
-
D-Allose
mutant N452T, pH 7.0
Acinetobacter calcoaceticus
46.5
-
D-maltose
mutant N452T, pH 7.0
Acinetobacter calcoaceticus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-O-methyl-D-glucose + oxidized 2,6-dichlorophenolindophenol
81% of the activity with D-glucose
654398
Acinetobacter calcoaceticus
3-O-methyl-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-allose + oxidized 2,6-dichlorophenolindophenol
47% of the activity with D-glucose
654398
Acinetobacter calcoaceticus
D-allono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-galactose + oxidized 2,6-dichlorophenolindophenol
11% of the activity with D-glucose
654398
Acinetobacter calcoaceticus
D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-glucose + oxidized 2,6-dichlorophenolindophenol
-
654398
Acinetobacter calcoaceticus
D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
lactose + oxidized 2,6-dichlorophenolindophenol
61% of the activity with D-glucose
654398
Acinetobacter calcoaceticus
lactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
maltose + oxidized 2,6-dichlorophenolindophenol
61% of the activity with D-glucose
654398
Acinetobacter calcoaceticus
maltono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
69
-
D-galactose
mutant N462H, pH 7.0
Acinetobacter calcoaceticus
72
-
D-galactose
mutant N452T, pH 7.0
Acinetobacter calcoaceticus
232
-
D-galactose
wild-type, pH 7.0
Acinetobacter calcoaceticus
574
-
D-lactose
mutant N462H, pH 7.0
Acinetobacter calcoaceticus
588
-
D-maltose
mutant N462H, pH 7.0
Acinetobacter calcoaceticus
949
-
D-Allose
mutant N452T, pH 7.0
Acinetobacter calcoaceticus
1002
-
D-maltose
mutant N452T, pH 7.0
Acinetobacter calcoaceticus
1035
-
D-Allose
mutant N462H, pH 7.0
Acinetobacter calcoaceticus
1038
-
D-lactose
mutant N452T, pH 7.0
Acinetobacter calcoaceticus
1064
-
3-O-methyl-D-glucose
mutant N462H, pH 7.0
Acinetobacter calcoaceticus
1253
-
3-O-methyl-D-glucose
mutant N452T, pH 7.0
Acinetobacter calcoaceticus
1399
-
D-glucose
mutant N462H, pH 7.0
Acinetobacter calcoaceticus
1659
-
D-lactose
wild-type, pH 7.0
Acinetobacter calcoaceticus
1791
-
D-glucose
mutant N452T, pH 7.0
Acinetobacter calcoaceticus
1930
-
D-maltose
wild-type, pH 7.0
Acinetobacter calcoaceticus
2509
-
D-Allose
wild-type, pH 7.0
Acinetobacter calcoaceticus
3011
-
3-O-methyl-D-glucose
wild-type, pH 7.0
Acinetobacter calcoaceticus
3860
-
D-glucose
wild-type, pH 7.0
Acinetobacter calcoaceticus
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
22
-
D-maltose
mutant N452T, pH 7.0
Acinetobacter calcoaceticus
23
-
D-galactose
mutant N452T, pH 7.0
Acinetobacter calcoaceticus
25
-
D-Allose
mutant N452T, pH 7.0
Acinetobacter calcoaceticus
26
-
D-galactose
mutant N462H, pH 7.0
Acinetobacter calcoaceticus
31
-
D-lactose
mutant N452T, pH 7.0
Acinetobacter calcoaceticus
32
-
D-Allose
mutant N462H, pH 7.0
Acinetobacter calcoaceticus
32
-
D-lactose
mutant N462H, pH 7.0
Acinetobacter calcoaceticus
37
-
3-O-methyl-D-glucose
mutant N462H, pH 7.0
Acinetobacter calcoaceticus
37
-
D-maltose
mutant N462H, pH 7.0
Acinetobacter calcoaceticus
44
-
D-galactose
wild-type, pH 7.0
Acinetobacter calcoaceticus
45
-
3-O-methyl-D-glucose
mutant N452T, pH 7.0
Acinetobacter calcoaceticus
71
-
D-Allose
wild-type, pH 7.0
Acinetobacter calcoaceticus
74
-
D-maltose
wild-type, pH 7.0
Acinetobacter calcoaceticus
88
-
D-lactose
wild-type, pH 7.0
Acinetobacter calcoaceticus
105
-
3-O-methyl-D-glucose
wild-type, pH 7.0
Acinetobacter calcoaceticus
114
-
D-glucose
mutant N462H, pH 7.0
Acinetobacter calcoaceticus
143
-
D-glucose
mutant N452T, pH 7.0
Acinetobacter calcoaceticus
154
-
D-glucose
wild-type, pH 7.0
Acinetobacter calcoaceticus
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
22
-
D-maltose
mutant N452T, pH 7.0
Acinetobacter calcoaceticus
23
-
D-galactose
mutant N452T, pH 7.0
Acinetobacter calcoaceticus
25
-
D-Allose
mutant N452T, pH 7.0
Acinetobacter calcoaceticus
26
-
D-galactose
mutant N462H, pH 7.0
Acinetobacter calcoaceticus
31
-
D-lactose
mutant N452T, pH 7.0
Acinetobacter calcoaceticus
32
-
D-Allose
mutant N462H, pH 7.0
Acinetobacter calcoaceticus
32
-
D-lactose
mutant N462H, pH 7.0
Acinetobacter calcoaceticus
37
-
3-O-methyl-D-glucose
mutant N462H, pH 7.0
Acinetobacter calcoaceticus
37
-
D-maltose
mutant N462H, pH 7.0
Acinetobacter calcoaceticus
44
-
D-galactose
wild-type, pH 7.0
Acinetobacter calcoaceticus
45
-
3-O-methyl-D-glucose
mutant N452T, pH 7.0
Acinetobacter calcoaceticus
71
-
D-Allose
wild-type, pH 7.0
Acinetobacter calcoaceticus
74
-
D-maltose
wild-type, pH 7.0
Acinetobacter calcoaceticus
88
-
D-lactose
wild-type, pH 7.0
Acinetobacter calcoaceticus
105
-
3-O-methyl-D-glucose
wild-type, pH 7.0
Acinetobacter calcoaceticus
114
-
D-glucose
mutant N462H, pH 7.0
Acinetobacter calcoaceticus
143
-
D-glucose
mutant N452T, pH 7.0
Acinetobacter calcoaceticus
154
-
D-glucose
wild-type, pH 7.0
Acinetobacter calcoaceticus
Other publictions for EC 1.1.99.35
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739914
An
Regulation of pyrroloquinoline ...
Pseudomonas putida, Pseudomonas putida KT 2240
Appl. Environ. Microbiol.
82
4955-4964
2016
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1
1
1
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42
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4
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1
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1
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1
1
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4
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1
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1
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739848
Flexer
Wired pyrroloquinoline quinone ...
Acinetobacter calcoaceticus
Anal. Chem.
86
2465-2473
2014
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2
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1
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1
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1
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739872
Ben Farhat
Coexpression of the pyrroloqui ...
Serratia marcescens, Serratia marcescens CTM 50650
Appl. Biochem. Biotechnol.
170
1738-1750
2013
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1
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4
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4
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739873
Stredansky
Monitoring of PQQ-dependent gl ...
Acinetobacter calcoaceticus
Appl. Biochem. Biotechnol.
171
1032-1041
2013
-
-
-
-
-
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-
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1
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13
-
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1
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1
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13
-
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12
12
724836
Schubart
-
A pyrroloquinolinequinone-depe ...
Acinetobacter calcoaceticus
Electrochim. Acta
82
224-232
2012
-
1
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1
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1
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1
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1
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1
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1
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1
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724852
Yu
-
High-level production of solub ...
Acinetobacter calcoaceticus, Acinetobacter calcoaceticus L.M.D. 79.41
Eng. Life Sci.
12
574-582
2012
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1
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10
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1
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1
1
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1
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1
10
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1
2
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10
1
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1
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723943
Flexer
Efficient direct electron tran ...
Acinetobacter calcoaceticus
Anal. Chem.
83
5721-5727
2011
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1
1
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1
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2
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1
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1
1
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1
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1
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2
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724172
Durand
Designing a highly active solu ...
Acinetobacter calcoaceticus
Biochem. Biophys. Res. Commun.
402
750-754
2010
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1
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1
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2
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1
1
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1
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1
1
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1
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1
1
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1
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2
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1
1
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-
1
1
-
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-
-
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-
655127
Tanaka
Increasing stability of water- ...
Acinetobacter calcoaceticus
BMC Biochem.
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1
2005
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3
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2
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1
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1
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5
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3
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2
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1
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5
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655929
Igarashi
Construction and characterizat ...
Acinetobacter calcoaceticus
J. Biochem. Biophys. Methods
61
331-338
2004
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1
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1
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11
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1
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6
1
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1
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1
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11
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6
1
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654863
Oubrie
Structure and mechanism of sol ...
Acinetobacter calcoaceticus
Biochim. Biophys. Acta
1647
143-151
2003
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1
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1
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1
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1
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1
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-
654398
Sode
-
Construction of engineered wat ...
Acinetobacter calcoaceticus
Biocatal. Biotransform.
20
405-412
2002
-
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5
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18
-
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1
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6
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18
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5
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18
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6
-
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18
-
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-
18
18
655363
Yoshida
-
Secretion of water soluble pyr ...
Acinetobacter calcoaceticus
Enzyme Microb. Technol.
30
312-318
2002
-
1
1
-
1
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4
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1
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1
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2
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1
1
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1
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4
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1
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2
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639218
Sode
Increasing the thermal stabili ...
Acinetobacter calcoaceticus
Enzyme Microb. Technol.
26
491-496
2000
-
-
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-
7
-
-
2
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1
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2
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9
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1
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7
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2
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2
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9
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1
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-
702202
Dewanti
Ca2+-assisted, direct hydride ...
Acinetobacter calcoaceticus
Biochemistry
39
9384-9392
2000
-
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2
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1
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1
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1
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1
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702811
Kojima
-
The production of soluble pyrr ...
Acinetobacter calcoaceticus
Biotechnol. Lett.
22
1343-1347
2000
-
1
1
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1
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1
1
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639204
Oubrie
The 1.7 A crystal structure of ...
Acinetobacter calcoaceticus
J. Mol. Biol.
289
319-333
1999
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1
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1
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1
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1
1
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1
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639216
Igarashi
Construction and characterizat ...
Acinetobacter calcoaceticus
Biochem. Biophys. Res. Commun.
264
820-824
1999
-
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12
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16
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1
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9
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18
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12
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16
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9
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18
-
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18
18
639220
Oubrie
Active-site structure of the s ...
Acinetobacter calcoaceticus
Proc. Natl. Acad. Sci. USA
96
11787-11791
1999
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1
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1
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2
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1
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1
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639209
Olsthoorn
On the mechanism and specifici ...
Acinetobacter calcoaceticus
Biochemistry
37
13854-13861
1998
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-
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1
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16
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1
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16
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1
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17
17
703532
Olsthoorn
Negative cooperativity in the ...
Acinetobacter calcoaceticus
Eur. J. Biochem.
255
255-261
1998
-
-
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2
1
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2
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2
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1
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4
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3
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2
3
1
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2
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4
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703530
Olsthoorn
Ca2+ and its substitutes have ...
Acinetobacter calcoaceticus
Eur. J. Biochem.
247
659-665
1997
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1
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1
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702726
Matsushita
-
Soluble and membrane-bound qui ...
Acinetobacter calcoaceticus
Biosci. Biotechnol. Biochem.
59
1548-1555
1995
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5
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1
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1
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1
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5
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705107
Schlunegger
Crystallization and preliminar ...
Acinetobacter calcoaceticus
J. Mol. Biol.
233
784-786
1993
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1
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1
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1
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