Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibitory: cyanide | Pseudomonas sp. MA-1 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0084 | - |
4-pyridoxate | electron acceptor phenazine methosulfate, pH 8.0, 25°C | Pseudomonas sp. MA-1 | |
0.0093 | - |
4-pyridoxate | electron acceptor 2,6-dichloroindophenol, pH 8.0, 25°C | Pseudomonas sp. MA-1 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
undenatured preparations aggregate readily, leading to mass values of 148,000, 470,000, and more than 670000 obtained by density gradient centrifugation or by gel filtration | Pseudomonas sp. MA-1 |
61000 | - |
and 63000, PAGE | Pseudomonas sp. MA-1 |
63000 | - |
and 61000, PAGE | Pseudomonas sp. MA-1 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. MA-1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
from Pseudomonas MA-1 grown with pyridoxine as a sole source of carbon and nitrogen | Pseudomonas sp. MA-1 |
Storage Stability | Organism |
---|---|
4°C, half-life 3 days in absence of glycerol. In presence of glycerol 0-20% of the activity is lost in 1 week | Pseudomonas sp. MA-1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-pyridoxate + 2,6-dichloroindophenol | - |
Pseudomonas sp. MA-1 | 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate + reduced 2,6-dichloroindophenol | - |
? | |
4-pyridoxate + phenazine methosulfate | - |
Pseudomonas sp. MA-1 | 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate + reduced phenazine methosulfate | - |
? | |
additional information | 2,6-dichloroindophenol, phenazine methosulfate, and menadione are effective electron acceptors. Ubiquinones are less active, while NAD, FAD, and O2 are inactive. In membrane fractions, oxygen supports 4-pyridoxic acid oxidation via a CN--sensitive electron transport chain | Pseudomonas sp. MA-1 | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Pseudomonas sp. MA-1 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Pseudomonas sp. MA-1 |