BRENDA - Enzyme Database show
show all sequences of 1.1.99.B3

Xylo- and cello-oligosaccharide oxidation by gluco-oligosaccharide oxidase from Sarocladium strictum and variants with reduced substrate inhibition

Vuong, T.V.; Vesterinen, A.H.; Foumani, M.; Juvonen, M.; Seppaelae, J.; Tenkanen, M.; Master, E.R.; Biotechnol. Biofuels 6, 148 (2013)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
A38V
the mutation significantly increases the kcat and catalytic efficiency of the enzyme on oligosaccharides compared to the wild type enzyme
Sarocladium strictum
A38V/S388N
the mutation increases the kcat and catalytic efficiency of the enzyme on oligosaccharides compared to the wild type enzyme
Sarocladium strictum
E247A
the mutation decreases the kcat and catalytic efficiency of the enzyme on oligosaccharides compared to the wild type enzyme
Sarocladium strictum
Q353A
the mutation decreases the kcat and catalytic efficiency of the enzyme on oligosaccharides compared to the wild type enzyme
Sarocladium strictum
Q353N
the mutation decreases the kcat and catalytic efficiency of the enzyme on oligosaccharides compared to the wild type enzyme
Sarocladium strictum
Q384A
the mutation decreases the kcat and catalytic efficiency of the enzyme on oligosaccharides compared to the wild type enzyme
Sarocladium strictum
Q384N
the mutation decreases the kcat and catalytic efficiency of the enzyme on oligosaccharides compared to the wild type enzyme
Sarocladium strictum
W351A
the mutation decreases the kcat and catalytic efficiency of the enzyme on oligosaccharides compared to the wild type enzyme; the mutation increases Km values by up to 2 orders of magnitude while also increasing kcat up to 3fold on cello- and xylo-oligosaccharides and showing no substrate inhibition compared to the wild type enzyme
Sarocladium strictum
Y72A
the mutation decreases the kcat and catalytic efficiency of the enzyme on oligosaccharides compared to the wild type enzyme
Sarocladium strictum
Y72F
the mutation decreases the kcat and catalytic efficiency of the enzyme on oligosaccharides compared to the wild type enzyme
Sarocladium strictum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.005
-
xylohexaose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
0.039
-
cellobiose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
0.04
-
cellobiose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
0.05
-
cellobiose
mutant enzyme A38V/S388N, at pH 8.0 and 37C
Sarocladium strictum
0.05
-
cellotriose
mutant enzyme A38V, at pH 8.0 and 37C; wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
0.05
-
xylobiose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
0.05
-
xylopentaose
mutant enzyme A38V, at pH 8.0 and 37C; wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
0.052
-
alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
0.057
-
Cellopentaose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
0.057
-
xylobiose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
0.059
-
alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
0.06
-
Cellopentaose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
0.06
-
Cellotetraose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
0.06
-
xylohexaose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
0.067
-
xylotetraose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
0.07
-
Cellotetraose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
0.07
-
xylotetraose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
0.078
-
alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
0.08
-
cellohexaose
mutant enzyme A38V, at pH 8.0 and 37C; wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
0.08
-
cellotriose
mutant enzyme A38V/S388N, at pH 8.0 and 37C
Sarocladium strictum
0.08
-
xylotriose
mutant enzyme A38V, at pH 8.0 and 37C; wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
0.09
-
alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
0.09
-
Cellopentaose
mutant enzyme A38V/S388N, at pH 8.0 and 37C
Sarocladium strictum
0.09
-
Cellotetraose
mutant enzyme A38V/S388N, at pH 8.0 and 37C
Sarocladium strictum
0.099
-
xylobiose
mutant enzyme A38V/S388N, at pH 8.0 and 37C
Sarocladium strictum
0.1
-
cellohexaose
mutant enzyme A38V/S388N, at pH 8.0 and 37C
Sarocladium strictum
0.1
-
xylohexaose
mutant enzyme A38V/S388N, at pH 8.0 and 37C
Sarocladium strictum
0.1
-
xylotriose
mutant enzyme A38V/S388N, at pH 8.0 and 37C
Sarocladium strictum
0.104
-
xylopentaose
mutant enzyme A38V/S388N, at pH 8.0 and 37C
Sarocladium strictum
0.11
-
xylotetraose
mutant enzyme A38V/S388N, at pH 8.0 and 37C
Sarocladium strictum
0.3
-
4-O-methyl-alpha-D-glucopyranosyl uronic acid-(1->2)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
12
-
D-glucose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
15
-
D-glucose
mutant enzyme A38V/S388N, at pH 8.0 and 37C; wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
118
-
D-xylose
mutant enzyme A38V, at pH 8.0 and 37C; mutant enzyme A38V/S388N, at pH 8.0 and 37C
Sarocladium strictum
129
-
D-xylose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Sarocladium strictum
D7PF15
-
-
Sarocladium strictum CBS 346.70
D7PF15
-
-
Posttranslational Modification
Posttranslational Modification
Commentary
Organism
glycoprotein
-
Sarocladium strictum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-O-methyl-alpha-D-glucopyranosyl uronic acid-(1->2)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose + O2
-
740204
Sarocladium strictum
4-O-methyl-alpha-D-glucopyranosyl uronic acid-(1->2)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylono-1,5-lactone + H2O2
-
-
-
?
4-O-methyl-alpha-D-glucopyranosyl uronic acid-(1->2)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose + O2
-
740204
Sarocladium strictum CBS 346.70
4-O-methyl-alpha-D-glucopyranosyl uronic acid-(1->2)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylono-1,5-lactone + H2O2
-
-
-
?
alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose + O2
-
740204
Sarocladium strictum
alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylono-1,5-lactone + H2O2
-
-
-
?
alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose + O2
-
740204
Sarocladium strictum CBS 346.70
alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylono-1,5-lactone + H2O2
-
-
-
?
alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylose + O2
-
740204
Sarocladium strictum
alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylono-1,5-lactone + H2O2
-
-
-
?
alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylose + O2
-
740204
Sarocladium strictum CBS 346.70
alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylono-1,5-lactone + H2O2
-
-
-
?
cellobiose + O2
-
740204
Sarocladium strictum
cellobiono-1,5-lactone + H2O2
-
-
-
?
cellobiose + O2
-
740204
Sarocladium strictum CBS 346.70
cellobiono-1,5-lactone + H2O2
-
-
-
?
cellohexaose + O2
-
740204
Sarocladium strictum
cellohexaono-1,5-lactone + H2O2
-
-
-
?
cellopentaose + O2
-
740204
Sarocladium strictum
cellopentaono-1,5-lactone + H2O2
-
-
-
?
cellotetraose + O2
-
740204
Sarocladium strictum
cellotetraono-1,5-lactone + H2O2
-
-
-
?
cellotriose + O2
-
740204
Sarocladium strictum
cellotriono-1,5-lactone + H2O2
-
-
-
?
D-glucose + O2
-
740204
Sarocladium strictum
D-glucono-1,5-lactone + H2O2
-
-
-
?
D-glucose + O2
-
740204
Sarocladium strictum CBS 346.70
D-glucono-1,5-lactone + H2O2
-
-
-
?
D-xylose + O2
-
740204
Sarocladium strictum
D-xylono-1,5-lactone + H2O2
-
-
-
?
xylobiose + O2
highest activity
740204
Sarocladium strictum
xylobiono-1,5-lactone + H2O2
-
-
-
?
xylohexaose + O2
-
740204
Sarocladium strictum
xylohexaono-1,5-lactone + H2O2
-
-
-
?
xylopentaose + O2
-
740204
Sarocladium strictum
xylopentaono-1,5-lactone + H2O2
-
-
-
?
xylotetraose + O2
-
740204
Sarocladium strictum
xylotetraono-1,5-lactone + H2O2
-
-
-
?
xylotriose + O2
-
740204
Sarocladium strictum
xylotriono-1,5-lactone + H2O2
-
-
-
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
5.5
-
D-xylose
mutant enzyme A38V/S388N, at pH 8.0 and 37C
Sarocladium strictum
6
-
D-glucose
mutant enzyme A38V/S388N, at pH 8.0 and 37C
Sarocladium strictum
6.2
-
cellobiose
mutant enzyme A38V/S388N, at pH 8.0 and 37C
Sarocladium strictum
6.3
-
D-glucose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
6.6
-
cellohexaose
mutant enzyme A38V/S388N, at pH 8.0 and 37C
Sarocladium strictum
6.9
-
cellotriose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
7
-
cellobiose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
7.2
-
cellohexaose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
7.3
-
cellotriose
mutant enzyme A38V/S388N, at pH 8.0 and 37C
Sarocladium strictum
7.4
-
D-xylose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
7.5
-
xylobiose
mutant enzyme A38V/S388N, at pH 8.0 and 37C
Sarocladium strictum
7.6
-
Cellopentaose
mutant enzyme A38V/S388N, at pH 8.0 and 37C
Sarocladium strictum
7.8
-
Cellotetraose
mutant enzyme A38V/S388N, at pH 8.0 and 37C
Sarocladium strictum
7.8
-
xylohexaose
mutant enzyme A38V/S388N, at pH 8.0 and 37C
Sarocladium strictum
8.1
-
xylotetraose
mutant enzyme A38V/S388N, at pH 8.0 and 37C
Sarocladium strictum
8.3
-
Cellopentaose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
8.6
-
xylohexaose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
8.7
-
xylobiose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
8.7
-
xylotriose
mutant enzyme A38V/S388N, at pH 8.0 and 37C
Sarocladium strictum
8.8
-
alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
8.8
-
xylopentaose
mutant enzyme A38V/S388N, at pH 8.0 and 37C
Sarocladium strictum
9
-
alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
9.7
-
xylotetraose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
9.9
-
Cellotetraose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
10.3
-
xylotriose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
10.4
-
D-glucose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
10.4
-
xylopentaose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
11
-
4-O-methyl-alpha-D-glucopyranosyl uronic acid-(1->2)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
12
-
cellobiose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
12.3
-
alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
12.4
-
D-xylose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
12.7
-
Cellopentaose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
12.8
-
Cellotetraose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
13.2
-
cellohexaose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
13.5
-
alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
13.7
-
cellotriose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
14.5
-
xylotriose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
14.8
-
xylobiose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
15.2
-
xylohexaose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
15.2
-
xylopentaose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
16
-
xylotetraose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
-
Sarocladium strictum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
-
Sarocladium strictum
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
A38V
the mutation significantly increases the kcat and catalytic efficiency of the enzyme on oligosaccharides compared to the wild type enzyme
Sarocladium strictum
A38V/S388N
the mutation increases the kcat and catalytic efficiency of the enzyme on oligosaccharides compared to the wild type enzyme
Sarocladium strictum
E247A
the mutation decreases the kcat and catalytic efficiency of the enzyme on oligosaccharides compared to the wild type enzyme
Sarocladium strictum
Q353A
the mutation decreases the kcat and catalytic efficiency of the enzyme on oligosaccharides compared to the wild type enzyme
Sarocladium strictum
Q353N
the mutation decreases the kcat and catalytic efficiency of the enzyme on oligosaccharides compared to the wild type enzyme
Sarocladium strictum
Q384A
the mutation decreases the kcat and catalytic efficiency of the enzyme on oligosaccharides compared to the wild type enzyme
Sarocladium strictum
Q384N
the mutation decreases the kcat and catalytic efficiency of the enzyme on oligosaccharides compared to the wild type enzyme
Sarocladium strictum
W351A
the mutation decreases the kcat and catalytic efficiency of the enzyme on oligosaccharides compared to the wild type enzyme; the mutation increases Km values by up to 2 orders of magnitude while also increasing kcat up to 3fold on cello- and xylo-oligosaccharides and showing no substrate inhibition compared to the wild type enzyme
Sarocladium strictum
Y72A
the mutation decreases the kcat and catalytic efficiency of the enzyme on oligosaccharides compared to the wild type enzyme
Sarocladium strictum
Y72F
the mutation decreases the kcat and catalytic efficiency of the enzyme on oligosaccharides compared to the wild type enzyme
Sarocladium strictum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.005
-
xylohexaose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
0.039
-
cellobiose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
0.04
-
cellobiose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
0.05
-
cellobiose
mutant enzyme A38V/S388N, at pH 8.0 and 37C
Sarocladium strictum
0.05
-
cellotriose
mutant enzyme A38V, at pH 8.0 and 37C; wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
0.05
-
xylobiose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
0.05
-
xylopentaose
mutant enzyme A38V, at pH 8.0 and 37C; wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
0.052
-
alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
0.057
-
Cellopentaose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
0.057
-
xylobiose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
0.059
-
alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
0.06
-
Cellopentaose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
0.06
-
Cellotetraose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
0.06
-
xylohexaose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
0.067
-
xylotetraose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
0.07
-
Cellotetraose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
0.07
-
xylotetraose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
0.078
-
alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
0.08
-
cellohexaose
mutant enzyme A38V, at pH 8.0 and 37C; wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
0.08
-
cellotriose
mutant enzyme A38V/S388N, at pH 8.0 and 37C
Sarocladium strictum
0.08
-
xylotriose
mutant enzyme A38V, at pH 8.0 and 37C; wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
0.09
-
alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
0.09
-
Cellopentaose
mutant enzyme A38V/S388N, at pH 8.0 and 37C
Sarocladium strictum
0.09
-
Cellotetraose
mutant enzyme A38V/S388N, at pH 8.0 and 37C
Sarocladium strictum
0.099
-
xylobiose
mutant enzyme A38V/S388N, at pH 8.0 and 37C
Sarocladium strictum
0.1
-
cellohexaose
mutant enzyme A38V/S388N, at pH 8.0 and 37C
Sarocladium strictum
0.1
-
xylohexaose
mutant enzyme A38V/S388N, at pH 8.0 and 37C
Sarocladium strictum
0.1
-
xylotriose
mutant enzyme A38V/S388N, at pH 8.0 and 37C
Sarocladium strictum
0.104
-
xylopentaose
mutant enzyme A38V/S388N, at pH 8.0 and 37C
Sarocladium strictum
0.11
-
xylotetraose
mutant enzyme A38V/S388N, at pH 8.0 and 37C
Sarocladium strictum
0.3
-
4-O-methyl-alpha-D-glucopyranosyl uronic acid-(1->2)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
12
-
D-glucose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
15
-
D-glucose
mutant enzyme A38V/S388N, at pH 8.0 and 37C; wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
118
-
D-xylose
mutant enzyme A38V, at pH 8.0 and 37C; mutant enzyme A38V/S388N, at pH 8.0 and 37C
Sarocladium strictum
129
-
D-xylose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
Posttranslational Modification (protein specific)
Posttranslational Modification
Commentary
Organism
glycoprotein
-
Sarocladium strictum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-O-methyl-alpha-D-glucopyranosyl uronic acid-(1->2)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose + O2
-
740204
Sarocladium strictum
4-O-methyl-alpha-D-glucopyranosyl uronic acid-(1->2)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylono-1,5-lactone + H2O2
-
-
-
?
4-O-methyl-alpha-D-glucopyranosyl uronic acid-(1->2)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose + O2
-
740204
Sarocladium strictum CBS 346.70
4-O-methyl-alpha-D-glucopyranosyl uronic acid-(1->2)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylono-1,5-lactone + H2O2
-
-
-
?
alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose + O2
-
740204
Sarocladium strictum
alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylono-1,5-lactone + H2O2
-
-
-
?
alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose + O2
-
740204
Sarocladium strictum CBS 346.70
alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylono-1,5-lactone + H2O2
-
-
-
?
alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylose + O2
-
740204
Sarocladium strictum
alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylono-1,5-lactone + H2O2
-
-
-
?
alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylose + O2
-
740204
Sarocladium strictum CBS 346.70
alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylono-1,5-lactone + H2O2
-
-
-
?
cellobiose + O2
-
740204
Sarocladium strictum
cellobiono-1,5-lactone + H2O2
-
-
-
?
cellobiose + O2
-
740204
Sarocladium strictum CBS 346.70
cellobiono-1,5-lactone + H2O2
-
-
-
?
cellohexaose + O2
-
740204
Sarocladium strictum
cellohexaono-1,5-lactone + H2O2
-
-
-
?
cellopentaose + O2
-
740204
Sarocladium strictum
cellopentaono-1,5-lactone + H2O2
-
-
-
?
cellotetraose + O2
-
740204
Sarocladium strictum
cellotetraono-1,5-lactone + H2O2
-
-
-
?
cellotriose + O2
-
740204
Sarocladium strictum
cellotriono-1,5-lactone + H2O2
-
-
-
?
D-glucose + O2
-
740204
Sarocladium strictum
D-glucono-1,5-lactone + H2O2
-
-
-
?
D-glucose + O2
-
740204
Sarocladium strictum CBS 346.70
D-glucono-1,5-lactone + H2O2
-
-
-
?
D-xylose + O2
-
740204
Sarocladium strictum
D-xylono-1,5-lactone + H2O2
-
-
-
?
xylobiose + O2
highest activity
740204
Sarocladium strictum
xylobiono-1,5-lactone + H2O2
-
-
-
?
xylohexaose + O2
-
740204
Sarocladium strictum
xylohexaono-1,5-lactone + H2O2
-
-
-
?
xylopentaose + O2
-
740204
Sarocladium strictum
xylopentaono-1,5-lactone + H2O2
-
-
-
?
xylotetraose + O2
-
740204
Sarocladium strictum
xylotetraono-1,5-lactone + H2O2
-
-
-
?
xylotriose + O2
-
740204
Sarocladium strictum
xylotriono-1,5-lactone + H2O2
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
5.5
-
D-xylose
mutant enzyme A38V/S388N, at pH 8.0 and 37C
Sarocladium strictum
6
-
D-glucose
mutant enzyme A38V/S388N, at pH 8.0 and 37C
Sarocladium strictum
6.2
-
cellobiose
mutant enzyme A38V/S388N, at pH 8.0 and 37C
Sarocladium strictum
6.3
-
D-glucose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
6.6
-
cellohexaose
mutant enzyme A38V/S388N, at pH 8.0 and 37C
Sarocladium strictum
6.9
-
cellotriose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
7
-
cellobiose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
7.2
-
cellohexaose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
7.3
-
cellotriose
mutant enzyme A38V/S388N, at pH 8.0 and 37C
Sarocladium strictum
7.4
-
D-xylose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
7.5
-
xylobiose
mutant enzyme A38V/S388N, at pH 8.0 and 37C
Sarocladium strictum
7.6
-
Cellopentaose
mutant enzyme A38V/S388N, at pH 8.0 and 37C
Sarocladium strictum
7.8
-
Cellotetraose
mutant enzyme A38V/S388N, at pH 8.0 and 37C
Sarocladium strictum
7.8
-
xylohexaose
mutant enzyme A38V/S388N, at pH 8.0 and 37C
Sarocladium strictum
8.1
-
xylotetraose
mutant enzyme A38V/S388N, at pH 8.0 and 37C
Sarocladium strictum
8.3
-
Cellopentaose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
8.6
-
xylohexaose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
8.7
-
xylobiose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
8.7
-
xylotriose
mutant enzyme A38V/S388N, at pH 8.0 and 37C
Sarocladium strictum
8.8
-
alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
8.8
-
xylopentaose
mutant enzyme A38V/S388N, at pH 8.0 and 37C
Sarocladium strictum
9
-
alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
9.7
-
xylotetraose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
9.9
-
Cellotetraose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
10.3
-
xylotriose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
10.4
-
D-glucose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
10.4
-
xylopentaose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
11
-
4-O-methyl-alpha-D-glucopyranosyl uronic acid-(1->2)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
12
-
cellobiose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
12.3
-
alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
12.4
-
D-xylose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
12.7
-
Cellopentaose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
12.8
-
Cellotetraose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
13.2
-
cellohexaose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
13.5
-
alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
13.7
-
cellotriose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
14.5
-
xylotriose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
14.8
-
xylobiose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
15.2
-
xylohexaose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
15.2
-
xylopentaose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
16
-
xylotetraose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.05
-
D-xylose
mutant enzyme A38V/S388N, at pH 8.0 and 37C
Sarocladium strictum
0.06
-
D-xylose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
0.11
-
D-xylose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
0.4
-
D-glucose
mutant enzyme A38V/S388N, at pH 8.0 and 37C
Sarocladium strictum
0.42
-
D-glucose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
0.83
-
D-glucose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
36.5
-
4-O-methyl-alpha-D-glucopyranosyl uronic acid-(1->2)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
66.7
-
cellohexaose
mutant enzyme A38V/S388N, at pH 8.0 and 37C
Sarocladium strictum
73.3
-
xylohexaose
mutant enzyme A38V/S388N, at pH 8.0 and 37C
Sarocladium strictum
73.3
-
xylotetraose
mutant enzyme A38V/S388N, at pH 8.0 and 37C
Sarocladium strictum
75
-
xylobiose
mutant enzyme A38V/S388N, at pH 8.0 and 37C
Sarocladium strictum
78.3
-
Cellopentaose
mutant enzyme A38V/S388N, at pH 8.0 and 37C
Sarocladium strictum
85
-
xylopentaose
mutant enzyme A38V/S388N, at pH 8.0 and 37C
Sarocladium strictum
85
-
xylotriose
mutant enzyme A38V/S388N, at pH 8.0 and 37C
Sarocladium strictum
86.7
-
Cellotetraose
mutant enzyme A38V/S388N, at pH 8.0 and 37C
Sarocladium strictum
91.7
-
cellohexaose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
93.3
-
cellotriose
mutant enzyme A38V/S388N, at pH 8.0 and 37C
Sarocladium strictum
115
-
alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
116.7
-
cellobiose
mutant enzyme A38V/S388N, at pH 8.0 and 37C
Sarocladium strictum
135
-
xylohexaose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
136.7
-
xylotetraose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
136.7
-
xylotriose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
143.3
-
cellotriose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
145
-
Cellopentaose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
148.3
-
alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
150
-
alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
150
-
cellohexaose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
150
-
Cellotetraose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
151.7
-
xylobiose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
156.7
-
cellobiose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
183.3
-
xylotriose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
196.7
-
xylopentaose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
216.7
-
Cellopentaose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
233.3
-
alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
233.3
-
Cellotetraose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
233.3
-
xylotetraose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
250
-
cellotriose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
283.3
-
xylopentaose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
300
-
xylobiose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
300
-
xylohexaose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
306.7
-
cellobiose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.05
-
D-xylose
mutant enzyme A38V/S388N, at pH 8.0 and 37C
Sarocladium strictum
0.06
-
D-xylose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
0.11
-
D-xylose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
0.4
-
D-glucose
mutant enzyme A38V/S388N, at pH 8.0 and 37C
Sarocladium strictum
0.42
-
D-glucose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
0.83
-
D-glucose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
36.5
-
4-O-methyl-alpha-D-glucopyranosyl uronic acid-(1->2)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
66.7
-
cellohexaose
mutant enzyme A38V/S388N, at pH 8.0 and 37C
Sarocladium strictum
73.3
-
xylohexaose
mutant enzyme A38V/S388N, at pH 8.0 and 37C
Sarocladium strictum
73.3
-
xylotetraose
mutant enzyme A38V/S388N, at pH 8.0 and 37C
Sarocladium strictum
75
-
xylobiose
mutant enzyme A38V/S388N, at pH 8.0 and 37C
Sarocladium strictum
78.3
-
Cellopentaose
mutant enzyme A38V/S388N, at pH 8.0 and 37C
Sarocladium strictum
85
-
xylopentaose
mutant enzyme A38V/S388N, at pH 8.0 and 37C
Sarocladium strictum
85
-
xylotriose
mutant enzyme A38V/S388N, at pH 8.0 and 37C
Sarocladium strictum
86.7
-
Cellotetraose
mutant enzyme A38V/S388N, at pH 8.0 and 37C
Sarocladium strictum
91.7
-
cellohexaose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
93.3
-
cellotriose
mutant enzyme A38V/S388N, at pH 8.0 and 37C
Sarocladium strictum
115
-
alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
116.7
-
cellobiose
mutant enzyme A38V/S388N, at pH 8.0 and 37C
Sarocladium strictum
135
-
xylohexaose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
136.7
-
xylotetraose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
136.7
-
xylotriose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
143.3
-
cellotriose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
145
-
Cellopentaose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
148.3
-
alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
150
-
alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
150
-
cellohexaose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
150
-
Cellotetraose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
151.7
-
xylobiose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
156.7
-
cellobiose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
183.3
-
xylotriose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
196.7
-
xylopentaose
wild type enzyme, at pH 8.0 and 37C
Sarocladium strictum
216.7
-
Cellopentaose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
233.3
-
alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
233.3
-
Cellotetraose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
233.3
-
xylotetraose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
250
-
cellotriose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
283.3
-
xylopentaose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
300
-
xylobiose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
300
-
xylohexaose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
306.7
-
cellobiose
mutant enzyme A38V, at pH 8.0 and 37C
Sarocladium strictum
Other publictions for EC 1.1.99.B3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741429
Vuong
Direct comparison of gluco-oli ...
Sarocladium strictum
Sci. Rep.
6
37356
2016
-
-
1
-
1
-
1
6
-
-
-
-
-
1
-
-
1
-
-
-
-
-
11
-
-
-
-
6
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
1
-
6
-
-
-
-
-
-
-
1
-
-
-
-
11
-
-
-
-
6
-
-
-
-
-
-
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