Activating Compound | Comment | Organism | Structure |
---|---|---|---|
SDS | enzyme is optimally activated by 10 mM SDS | Vigna unguiculata |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 10 mM, 15% inhibition | Vigna unguiculata | |
resorcinol | 10 mM, 40% inhibition | Vigna unguiculata | |
Sodium azide | 10 mM, 100% inhibition | Vigna unguiculata | |
tropolone | most powerful specific PPO inhibitor. It reduces the PPO activity by 50%, when used at a low 0.01 mM concentration | Vigna unguiculata |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.44 | - |
4-methylcatechol | - |
Vigna unguiculata | |
9.86 | - |
4-methylcatechol | - |
Vigna unguiculata |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
by partially denaturing SDS-PAGE multiple molecular forms of active PPO are detected | Vigna unguiculata |
73000 | - |
by partially denaturing SDS-PAGE multiple molecular forms of active PPO are detected | Vigna unguiculata |
220000 | - |
by partially denaturing SDS-PAGE multiple molecular forms of active PPO are detected | Vigna unguiculata |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vigna unguiculata | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using (NH4)2SO4 precipitation and ion-exchange chromatography | Vigna unguiculata |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seedling | polyphenol oxidases are induced in cowpea plants by wounding | Vigna unguiculata | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
polyphenol oxidases are induced in cowpea plants by wounding. The highest activity levels are detected 48 h after this stimulus in both wounded and neighbor-to-wounded unifoliates of cowpea seedlings. The increase of activity is in the order of 13 to 15fold, respectively, in comparison to control unifoliates | Vigna unguiculata |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(-)-catechin + O2 | enzyme activity relative to 4-methylcatechol: 0.77% | Vigna unguiculata | ? | - |
? | |
2 catechol + O2 | enzyme activity relative to 4-methylcatechol: 87% | Vigna unguiculata | 2 1,2-benzoquinone + 2 H2O | - |
? | |
3-(3,4-dihydroxyphenyl)-L-alanine + O2 | enzyme activity relative to 4-methylcatechol: 1.15% | Vigna unguiculata | ? | - |
? | |
4-methylcatechol + O2 | enzyme shows highest activity with 4-methylcatechol | Vigna unguiculata | ? | - |
? | |
chlorogenic acid + O2 | enzyme activity relative to 4-methylcatechol: 6.15% | Vigna unguiculata | ? | - |
? | |
pyrogallol + O2 | enzyme activity relative to 4-methylcatechol: 9.24% | Vigna unguiculata | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
multimer | by partially denaturing SDS-PAGE multiple molecular forms of active PPO are detected: 58 kDa, 73 kDa and 220 kDa | Vigna unguiculata |
Synonyms | Comment | Organism |
---|---|---|
polyphenol oxidase | - |
Vigna unguiculata |
PPO | - |
Vigna unguiculata |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
enzyme is highly stable even 5 min at 80°C | Vigna unguiculata |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay at | Vigna unguiculata |
6 | - |
optimal pH using 4-methylcatechol as substrate | Vigna unguiculata |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8 | - |
Vigna unguiculata |
General Information | Comment | Organism |
---|---|---|
physiological function | polyphenol oxidases are induced in cowpea plants by wounding | Vigna unguiculata |