KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | analysis of enzyme dynamics and catalytic, spectral properties. Analysis of the structural state of the purified native laccase in solution at the phase transition before freezing, at freezing, and subsequent thawing of enzyme solution, processes in the active site on environmental temperature changes, overview | Trametes hirsuta |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Trametes hirsuta | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | the structure of laccase active sites including all copper centers of types T1, T2, and T3 changes during the phase transition, overview | Trametes hirsuta |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trametes hirsuta | - |
strain 072 | - |
Trametes hirsuta 72 | - |
strain 072 | - |
Purification (Comment) | Organism |
---|---|
native extracellular enzyme by a three-stage chromatographic purification to homogeneity | Trametes hirsuta |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
syringaldazine + O2 | - |
Trametes hirsuta | ? | - |
? | |
syringaldazine + O2 | - |
Trametes hirsuta 72 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | laccase exists in solution as a mixture of monomeric and aggregated particles in the percent ratio 85:15, the ratio does not change on phase transitions, small-angle X-ray scattering, overview | Trametes hirsuta |
More | the structure of laccase active sites including all copper centers of types T1, T2, and T3 changes during the phase transition, overview | Trametes hirsuta |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
assay at | Trametes hirsuta |